Galanthus xvalentinei (J. Allen) Beck nothosubsp. subplicatus (N. Zeybek) A. P. Davis (Amaryllidaceae) is an endemic hybrid between G. nivalis L. and G. plicatus M. Bieb. subsp. byzantinus (Baker) D. A. Webb. Lycorine, a common alkaloid found in Amaryllidaceae plants, has been shown to display important biological activities. In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. xvalentinei nothosubsp. subplicatus. A simple method for the extraction of lycorine in lowmass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut®). The chromatographic separation was carried out using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:95:5) applied at a flow rate of 1 mL min−1 using diode array dedector. The content of lycorine in the bulbs of G. xvalentinei nothosubsp. subplicatus was found to be 0.0028%, however, it was not detected in the aerial parts of this hybrid.
[1]. A.P. Davis 2006 M. Bishop A.P. Davis J. Grimshaw Snowdrops. A Monograph of Cultivated Galanthus Griffin Press Publishing Ltd. Cheltenham 9–11.
[2]. A.P. Davis A. Byfield N. Ozhatay K. Taylor 2001 Kew Bull. 56 639.
[3]. O. Hoshino 1998 G.A. Cordell The Alkaloids Chemistry and Biology Academic Press New York 323–424.
[4]. L. Szlávik A. Gyuris J. Minárovits P. Forgo J. Molnár J. Hohmann 2004 Planta Med. 70 871.
[5]. B. Weniger L. Italiano J.P. Beck J. Bastida S. Bergaňon C. Codina A. Lobstein R. Anton 1995 Planta Med. 61 77.
[6]. B. Sener I. Orhan J. Satajavivad 2003 Phytother. Res. 17 1220.
[7]. G.I. Kaya A. Fillik Y. Hısıl N. Unver 2004 Turkish J. Pharm. Sci. 1 105.
[8]. B. Bozkurt Sarıkaya , N. Unver Somer, M.A. Onur, Turkish J. Pharm. Sci., 9, 2012 (in press).
[9]. G.I. Kaya D. Cicek B. Sarıkaya M.A. Onur 2010 N. Unver Somer, Nat. Prod. Commun. 5 873.
[10]. G. Saltan Citoglu B. Sever Yılmaz O. Bahadır 2008 Chem. Nat. Comp. 44 826.
[11]. G.I. Kaya N. Unver B. Gözler J. Bastida 2004 Biochem. Syst. Ecol. 32 1059.
[12]. N.R. Mustafa I.K. Rhee R. Verpoorte 2003 J. Liq. Chromatogr. 26 3217.
[13]. S. Berkov J. Bastida F. Viladomat C. Codina 2008 Phytochem. Anal. 19 285.