View More View Less
  • 1 School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do, Korea
  • | 2 College of Pharmacy, Catholic University of Daegu, Gyeongsan-si, Gyeongbuk, Korea
Full access

Summary

Capsaicin has been reported to exhibit an inhibitory effect on the P-glycoprotein (P-gp) function in vitro. To investigate its concentration-dependent effect in vivo, a sensitive assay that can characterize the absorption and disposition of capsaicin needs to be developed. This study reports the development of a sensitive LC-MS/MS assay for the determination of capsaicin in mouse plasma. The sample pretreatment involved a one-step extraction of 20 μL plasma with t-butyl methyl ether. Separations were achieved on a C18 column and the detection was performed on an LC-ESI-MS/MS by multiple reaction monitoring. The assay was linear over a wide concentration range from 0.325 to 650 ng mL−1 (r > 0.999), with a LLOQ of 0.325 ng mL−1. The developed method was applied to i.v. (dose 0.325 and 0.65 mg kg) and oral absorption (dose 40 mg kg) studies in mice. After i.v. injection, the t1/2,λz, Vz and CLs ranged from 0.13–0.16 h, 127.6–141.8 mL, and 547.3–775.4 mL/h, respectively. After oral administration, a secondary peak was observed and the terminal half-life was prolonged (1.51 h). Capsaicin was poorly absorbed, with the absolute oral bioavailability (F) ranging from 1.02% to 1.56%. The developed assay may be useful in studies where sample volumes are limited.

  • [1]. G.A. Cordell O.E. Araujo 1993 Ann. Pharmacother. 27 330.

  • [2]. M.J. Caterina M.A. Schumacher M. Tominaga T.A. Rosen J.D. Levine D. Julius 1997 Nature 389 816.

  • [3]. I. Tolan D. Ragoobirsingh E.Y.S.A. Morrison 2001 Phytother. Res. 15 391.

  • [4]. C.C. Chou Y.C. Wu Y.F. Wang M.J. Chou S.J. Kuo D.R. Chen 2009 Oncol. Rep. 21 665.

  • [5]. S.P. Huang J.C. Chen C.C. Wu C.T. Chen N.Y. Tang Y.T. Ho C. Lo J.P. Lin J.G. Chung J.G. Lin 2009 Anticancer Res. 29 165.

  • [6]. M.Y. Kim L.J. Trudel G.N. Wogan 2009 Anticancer Res. 29 3733.

  • [7]. K.C. Brown T.R. Witte W.E. Hardman H. Luo Y.C. Chen A.B. Carpenter J.K. Lau P. Dasgupta 2010 PLos ONE 5 1.

  • [8]. T. Nabekura S. Kamiyama S. Kitagawa 2005 Biochem. Biophys. Res Commun. 327 866.

  • [9]. O.M.E.A. Salam O.A. Heikal S. El-Shenawy 2005 Pharmacology 73 121.

  • [10]. D. Suresh K. Srivasan 2010 Indian J. Med. Res. 131 682.

  • [11]. J. Lu M. Cwik 1997 J. Chromatogr. B 701 135.

  • [12]. R. Wolf C. Huschka K. Raith W. Wohlrab R.H.H. Neubert 1999 J. Liquid Chromatogr. Rel. Technol. 22 531.

  • [13]. C.A. Reilly D.J. Crouch G.S. Yost A.A. Fatah 2002 J. Anal. Toxicol. 26 313.

  • [14]. S. Babbar J.F. Marier M.S. Moukassi M. Beliveau G.F. Vanhove S. Chanda K. Bley 2009 Ther. Drug Monit. 31 502.

  • [15]. Q. Zhang J. Hu L. Sheng Y. Li 2010 J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 878 2292.

  • [16]. S. Chanda M. Bashir S. Babbar A. Koganti K. Bley 2008 Drug Metab. Dispos. 36 670.

  • [17]. B.S. Shin S.W. Hwang J.B. Bulitta J.B. Lee S.D. Yang J.S. Park M.C. Kwon D.J. Kim H.S. Yoon S.D. Yoo 2010 J. Toxicol. Environ. Health-Part A 73 1586.

    • Search Google Scholar
    • Export Citation
The author instruction is available in PDF.
Please, download the file from HERE.
 
The Open Access statement together with the description of the Copyright and License Policy are available in PDF.
Please, download the file from HERE.
 

 

Senior editors

Editor(s)-in-Chief: Kowalska, Teresa

Editor(s)-in-Chief: Sajewicz, Mieczyslaw

Editors(s) Danica Agbaba (University of Belgrade, Belgrade, Serbia);
Ivana Stanimirova-Daszykowska (University of Silesia, Katowice, Poland),
Monika Waksmundzka-Hajnos (Medical University of Lublin, Lublin, Poland)

Editorial Board

R. Bhushan (The Indian Institute of Technology, Roorkee, India)
J. Bojarski (Jagiellonian University, Kraków, Poland)
B. Chankvetadze (State University of Tbilisi, Tbilisi, Georgia)
M. Daszykowski (University of Silesia, Katowice, Poland)
T.H. Dzido (Medical University of Lublin, Lublin, Poland)
A. Felinger (University of Pécs, Pécs, Hungary)
K. Glowniak (Medical University of Lublin, Lublin, Poland)
B. Glód (Siedlce University of Natural Sciences and Humanities, Siedlce, Poland)
K. Kaczmarski (Rzeszow University of Technology, Rzeszów, Poland)
H. Kalász (Semmelweis University, Budapest, Hungary)
I. Klebovich (Semmelweis University, Budapest, Hungary)
A. Koch (Private Pharmacy, Hamburg, Germany)
Ł. Komsta (Medical University of Lublin, Lublin, Poland)
P. Kus (Univerity of Silesia, Katowice, Poland)
D. Mangelings (Free University of Brussels, Brussels, Belgium)
E. Mincsovics (Corvinus University of Budapest, Budapest, Hungary)
G. Morlock (Giessen University, Giessen, Germany)
J. Sherma (Lafayette College, Easton, PA, USA)
R. Skibiński (Medical University of Lublin, Lublin, Poland)
B. Spangenberg (Offenburg University of Applied Sciences, Germany)
T. Tuzimski (Medical University of Lublin, Lublin, Poland)
Y. Vander Heyden (Free University of Brussels, Brussels, Belgium)
A. Voelkel (Poznań University of Technology, Poznań, Poland)
B. Walczak (University of Silesia, Katowice, Poland)
W. Wasiak (Adam Mickiewicz University, Poznań, Poland)

KOWALSKA, TERESA
E-mail: kowalska@us.edu.pl

SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search
  • Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences
  • SCOPUS
  • GoogleScholar
  • Index Copernicus
  • CABI
2020
 
Total Cites
650
WoS
Journal
Impact Factor
1,639
Rank by
Chemistry, Analytical 71/83 (Q4)
Impact Factor
 
Impact Factor
1,412
without
Journal Self Cites
5 Year
1,301
Impact Factor
Journal
0,34
Citation Indicator
 
Rank by Journal
Chemistry, Analytical 75/93 (Q4)
Citation Indicator
 
Citable
45
Items
Total
43
Articles
Total
2
Reviews
Scimago
28
H-index
Scimago
0,316
Journal Rank
Scimago
Chemistry (miscellaneous) Q3
Quartile Score
 
Scopus
393/181=2,2
Scite Score
 
Scopus
General Chemistry 215/398 (Q3)
Scite Score Rank
 
Scopus
0,560
SNIP
 
Days from
58
sumbission
 
to acceptance
 
Days from
68
acceptance
 
to publication
 
Acceptance
51%
Rate

2019  
Total Cites
WoS
495
Impact Factor 1,418
Impact Factor
without
Journal Self Cites
1,374
5 Year
Impact Factor
0,936
Immediacy
Index
0,460
Citable
Items
50
Total
Articles
50
Total
Reviews
0
Cited
Half-Life
6,2
Citing
Half-Life
8,3
Eigenfactor
Score
0,00048
Article Influence
Score
0,164
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,05895
Average
IF
Percentile
20,349
Scimago
H-index
26
Scimago
Journal Rank
0,255
Scopus
Scite Score
226/167=1,4
Scopus
Scite Score Rank
Chemistry (miscellaneous) 240/398 (Q3)
Scopus
SNIP
0,494
Acceptance
Rate
41%

 

Acta Chromatographica
Publication Model Online only
Gold Open Access
Submission Fee none
Article Processing Charge 400 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

Acta Chromatographica
Language English
Size A4
Year of
Foundation
1992
Publication
Programme
2021 Volume 33
Volumes
per Year
1
Issues
per Year
4
Founder Institute of Chemistry, University of Silesia
Founder's
Address
PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
May 2021 2 0 0
Jun 2021 0 0 0
Jul 2021 0 1 0
Aug 2021 0 4 0
Sep 2021 0 1 0
Oct 2021 0 2 0
Nov 2021 0 0 0