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D. Suchy
D. Suchy
Department of Internal Medicine and Clinical Pharmacology, Medical University of Silesia, Medyków 18, PL 40752, Katowice, Poland
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K. Łabuzek
K. Łabuzek
Department of Internal Medicine and Clinical Pharmacology, Medical University of Silesia, Medyków 18, PL 40752, Katowice, Poland
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O. Pierzchała
O. Pierzchała
Department of Internal Medicine and Clinical Pharmacology, Medical University of Silesia, Medyków 18, PL 40752, Katowice, Poland
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B. Okopień
B. Okopień
Department of Internal Medicine and Clinical Pharmacology, Medical University of Silesia, Medyków 18, PL 40752, Katowice, Poland
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Summary
Ezetimibe is the first in a new class of antihypercholesterolemic drugs. Since it has not long been available on the market, many of its properties may still be revealed. Analytical methods for its determination are scarce, especially regarding serum samples. A simple, fast, and effective high-performance liquid chromatography-ultraviolet (HPLC-UV) method for the determination of ezetimibe concentration in human serum has therefore been developed. Three mobile phases were analysed, and original modifications to the concentration and flow parameters were made. Of five potential internal standards (IS), only nitrendipine was found to be suitable. The analytical wavelength was chosen based on the absorption spectrum of ezetimibe in the mobile phase. Finally, an extraction analysis was performed using two different solvents, and the extrahent volume was optimised. The final method developed was as follows. Single extraction of 1 mL serum sample, spiked with IS, was performed using 10 mL of methyl-t-butyl ether. Separation was obtained at ambient temperature on a Waters C18 Symmetry Shield (4.6 mm × 250 mm, 5 μm) column. The isocratic mobile phase was composed of acetonitrile and 0.1 M ammonium acetate aqueous solution 55:45 (v/v), set at flow rate of 0.75 mL min−1. Ezetimibe was detected at a wavelength of 232 nm after 5.49 min, and the IS was detected at 8.05 min. The developed method has been validated according to ICH standards. It was found to be specific, precise, accurate and linear over the range 10–800 ng mL−1 with R2 > 0.998, and detection and quantification limits of 4.60 ng mL−1 and 13.94 ng mL−1, respectively. The method has been applied to clinical serum samples. The developed technique allowed for successful in vivo assessment of ezetimibe concentrations in samples obtained from hypercholesterolemia patients who are chronically receiving the drug.
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Senior editors
Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)
Editor(s)-in-Chief: Sajewicz, Mieczyslaw
Editors(s)
- Danica Agbaba (University of Belgrade, Belgrade, Serbia)
- Łukasz Komsta (Medical University of Lublin, Lublin, Poland)
- Ivana Stanimirova-Daszykowska (University of Silesia, Katowice, Poland)
- Monika Waksmundzka-Hajnos (Medical University of Lublin, Lublin, Poland)
Editorial Board
- R. Bhushan (The Indian Institute of Technology, Roorkee, India)
- J. Bojarski (Jagiellonian University, Kraków, Poland)
- B. Chankvetadze (State University of Tbilisi, Tbilisi, Georgia)
- M. Daszykowski (University of Silesia, Katowice, Poland)
- T.H. Dzido (Medical University of Lublin, Lublin, Poland)
- A. Felinger (University of Pécs, Pécs, Hungary)
- K. Glowniak (Medical University of Lublin, Lublin, Poland)
- B. Glód (Siedlce University of Natural Sciences and Humanities, Siedlce, Poland)
- A. Gumieniczek (Medical University of Lublin, Lublin, Poland)
- U. Hubicka (Jagiellonian University, Kraków, Poland)
- K. Kaczmarski (Rzeszow University of Technology, Rzeszów, Poland)
- H. Kalász (Semmelweis University, Budapest, Hungary)
- K. Karljiković Rajić (University of Belgrade, Belgrade, Serbia)
- I. Klebovich (Semmelweis University, Budapest, Hungary)
- A. Koch (Private Pharmacy, Hamburg, Germany)
- P. Kus (Univerity of Silesia, Katowice, Poland)
- D. Mangelings (Free University of Brussels, Brussels, Belgium)
- E. Mincsovics (Corvinus University of Budapest, Budapest, Hungary)
- Á. M. Móricz (Centre for Agricultural Research, Budapest, Hungary)
- G. Morlock (Giessen University, Giessen, Germany)
- A. Petruczynik (Medical University of Lublin, Lublin, Poland)
- R. Skibiński (Medical University of Lublin, Lublin, Poland)
- B. Spangenberg (Offenburg University of Applied Sciences, Germany)
- T. Tuzimski (Medical University of Lublin, Lublin, Poland)
- Y. Vander Heyden (Free University of Brussels, Brussels, Belgium)
- A. Voelkel (Poznań University of Technology, Poznań, Poland)
- B. Walczak (University of Silesia, Katowice, Poland)
- W. Wasiak (Adam Mickiewicz University, Poznań, Poland)
- I.G. Zenkevich (St. Petersburg State University, St. Petersburg, Russian Federation)
KOWALSKA, TERESA (1946-2023)
E-mail: kowalska@us.edu.pl
SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl
Indexing and Abstracting Services:
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- Sci Search
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- Chemistry Citation Index and Current Content/Physical
- Chemical and Earth Sciences
- SCOPUS
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- CABELLS Journalytics
| 2022 | |
|---|---|
| Web of Science | |
| Total Cites WoS |
647 |
| Journal Impact Factor | 1.9 |
| Rank by Impact Factor |
Chemistry, Analytical (Q3) |
| Impact Factor without Journal Self Cites |
1.9 |
| 5 Year Impact Factor |
1.4 |
| Journal Citation Indicator | 0.41 |
| Rank by Journal Citation Indicator |
Chemistry, Analytical (Q3) |
| Scimago | |
| Scimago H-index |
29 |
| Scimago Journal Rank |
0.28 |
| Scimago Quartile Score |
Chemistry (miscellaneous) (Q3) |
| Scopus | |
| Scopus Cite Score |
3.1 |
| Scopus CIte Score Rank |
General Chemistry 211/407 (48th PCTL) |
| Scopus SNIP |
0.549 |
| 2021 | |
|---|---|
| Web of Science | |
| Total Cites WoS |
652 |
| Journal Impact Factor | 2,011 |
| Rank by Impact Factor | Chemistry, Analytical 66/87 |
| Impact Factor without Journal Self Cites |
1,789 |
| 5 Year Impact Factor |
1,350 |
| Journal Citation Indicator | 0,40 |
| Rank by Journal Citation Indicator | Chemistry, Analytical 72/99 |
| Scimago | |
| Scimago H-index |
29 |
| Scimago Journal Rank |
0,27 |
| Scimago Quartile Score | Chemistry (miscellaneous) (Q3) |
| Scopus | |
| Scopus Cite Score |
2,8 |
| Scopus CIte Score Rank |
General Chemistry 210/409 (Q3) |
| Scopus SNIP |
0,586 |
|
2020
|
|
|
Total Cites
|
650
|
|
WoS
|
|
|
Journal
Impact Factor |
1,639
|
|
Rank by
|
Chemistry, Analytical 71/83 (Q4)
|
|
Impact Factor
|
|
|
Impact Factor
|
1,412
|
|
without
|
|
|
Journal Self Cites
|
|
|
5 Year
|
1,301
|
|
Impact Factor
|
|
|
Journal
|
0,34
|
|
Citation Indicator
|
|
|
Rank by Journal
|
Chemistry, Analytical 75/93 (Q4)
|
|
Citation Indicator
|
|
|
Citable
|
45
|
|
Items
|
|
|
Total
|
43
|
|
Articles
|
|
|
Total
|
2
|
|
Reviews
|
|
|
Scimago
|
28
|
|
H-index
|
|
|
Scimago
|
0,316
|
|
Journal Rank
|
|
|
Scimago
|
Chemistry (miscellaneous) Q3
|
|
Quartile Score
|
|
|
Scopus
|
393/181=2,2
|
|
Scite Score
|
|
|
Scopus
|
General Chemistry 215/398 (Q3)
|
|
Scite Score Rank
|
|
|
Scopus
|
0,560
|
|
SNIP
|
|
|
Days from
|
58
|
|
submission
|
|
|
to acceptance
|
|
|
Days from
|
68
|
|
acceptance
|
|
|
to publication
|
|
|
Acceptance
|
51%
|
| Rate |
| 2019 | |
|---|---|
| Total Cites WoS |
495 |
| Impact Factor | 1,418 |
| Impact Factor without Journal Self Cites |
1,374 |
| 5 Year Impact Factor |
0,936 |
| Immediacy Index |
0,460 |
| Citable Items |
50 |
| Total Articles |
50 |
| Total Reviews |
0 |
| Cited Half-Life |
6,2 |
| Citing Half-Life |
8,3 |
| Eigenfactor Score |
0,00048 |
| Article Influence Score |
0,164 |
| % Articles in Citable Items |
100,00 |
| Normalized Eigenfactor |
0,05895 |
| Average IF Percentile |
20,349 |
| Scimago H-index |
26 |
| Scimago Journal Rank |
0,255 |
| Scopus Scite Score |
226/167=1,4 |
| Scopus Scite Score Rank |
Chemistry (miscellaneous) 240/398 (Q3) |
| Scopus SNIP |
0,494 |
| Acceptance Rate |
41% |
| Acta Chromatographica | |
|---|---|
| Publication Model | Online only Gold Open Access |
| Submission Fee | none |
| Article Processing Charge | 400 EUR/article |
| Regional discounts on country of the funding agency | World Bank Lower-middle-income economies: 50% World Bank Low-income economies: 100% |
| Further Discounts | Editorial Board / Advisory Board members: 50% Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100% |
| Subscription Information | Gold Open Access |
| Purchase per Title | |
| Acta Chromatographica | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1988 |
| Volumes per Year |
1 |
| Issues per Year |
4 |
| Founder | Institute of Chemistry, University of Silesia |
| Founder's Address |
PL-40-007 Katowice, Poland, Bankowa 12 |
| Publisher | Akadémiai Kiadó |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Responsible Publisher |
Chief Executive Officer, Akadémiai Kiadó |
| ISSN | 2083-5736 (Online) |
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Apr 2023 | 0 | 2 | 3 |
| May 2023 | 0 | 1 | 2 |
| Jun 2023 | 0 | 2 | 5 |
| Jul 2023 | 0 | 1 | 3 |
| Aug 2023 | 0 | 1 | 1 |
| Sep 2023 | 0 | 6 | 2 |
| Oct 2023 | 0 | 0 | 0 |
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