Acta Chromatographica
Volume/Issue:
Volume 25: Issue 4
Determination of four kinds of endogenous hormones in poplar dialyzate by HPLC with microdialysis
Authors:
M. Jinlong Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China
Life Science College, Dalian Nationalities University, Dalian, 116600, China

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J. Guobin Environment and Resources College, Dalian Nationalities University, Dalian, 11660, China

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,
J. Bo Life Science College, Dalian Nationalities University, Dalian, 116600, China

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J. Hua Environment and Resources College, Dalian Nationalities University, Dalian, 11660, China

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Y. Shanjing Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China

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Pages:
627–637
Online Publication Date:
01 Dec 2013
Publication Date:
01 Dec 2013
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.25.2013.4.3
Keywords:
microdialysis; high-performance liquid chromatography; Populus wutunensis ; endogenous hormones
Full access

Summary

A dialyzate was obtained from the tender stems of living poplar (Populus wutunensis) by microdialysis (MD). Four kinds of endogenous hormones, namely, benzylamino purine, gibberellic acid, indole acetic acid, and abscisic acid, were then simultaneously determined by HPLC. The molecular weight cutoff (MWCO) of the MD probe was 30 kDa. The perfusion rate was 1 μL/min, and the perfusion liquid was Millipore-Q water. HPLC was performed using an Inertsil ODS-SP column. The mobile phase comprised 60:40 (v/v) methanol and water containing 0.2% phosphoric acid. The elution rate was 0.6 mL/min rate at 30°C. The average recoveries of the four kinds of hormones ranged from 93% to 109%, with correlation coefficients greater than 0.998. The determinations of endogenous hormones in plants as real time, living, and online were realized by MD-HPLC. The cells and macromolecules were excluded by the MD probe so centrifugation or protein precipitation was not required prior to analysis. MD-HPLC is a highly sensitive and selective method for the simultaneous profiling and quantification of a wide variety of plant hormone groups. This method is convenient for studying the mechanism of plant hormones.

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Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

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Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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