Acta Chromatographica
Volume/Issue:
Volume 26: Issue 2
Simple, fast, and sensitive isocratic high-performance liquid chromatography method for the quantification of latanoprost
Authors:
S. Mansoor School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, 30332-0100, USA

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C. Tas School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, 30332-0100, USA
Department of Pharmaceutical Sciences, Gulhane Military Medical Academy, 06018, Etlik, Ankara, Turkey

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Pages:
191–202
Online Publication Date:
01 Jun 2014
Publication Date:
01 Jun 2014
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.26.2014.2.1
Keywords:
latanoprost; commercial formulation; validation; triamcinolone acetonide; isocratic HPLC
Open access

Summary

A simple, sensitive, fast, and accurate isocratic high-performance liquid chromatography (HPLC) method with UV detection (205 nm) has been developed and validated for the quantification of latanoprost (in nanogram level) in pure and commercial dosage form. An Agilent Eclipse XDB-C18 column (150 × 4.6 mm i.d., 3.5 μm particle size) was used. Chromatography was performed with a mobile phase containing a mixture of acetonitrile and water (70:30, v/v) containing 0.1% v/v trifluoroacetic acid, adjusted at pH 3.0 at a flow rate of 1 mL min−1. Each analysis required 3.0 min including triamcinolone acetonide as an internal standard. The method was validated for linearity, accuracy, precision, specificity, system suitability, and robustness. The calibration curve was linear in the concentration range 0.0125–1 μg mL−1; the correlation coefficient was 0.999. The method proved to be accurate, precise, specific, rapid, and reproducible according to ICH standards. The method showed good recoveries (average 100.15 %), and the relative standard deviations of intra- and inter-day were <2.0%. This method is novel since there has been no report about analysis of latanoprost at ng mL−1 levels using a simple HPLC method. Besides regular quality control assay of latanoprost in raw material and in commercial formulations, this method has key importance for scientists working on sustained drug delivery system of latanoprost in which quantification in nanogram levels is one of the most important parameters.

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Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

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Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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