Acta Chromatographica
Volume/Issue:
Volume 26: Issue 2
Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC
Authors:
R. Parveen Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India

Search for other papers by R. Parveen in
Current site
Google Scholar
PubMed Close
,
F. J. Ahmad Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India

Search for other papers by F. J. Ahmad in
Current site
Google Scholar
PubMed Close
,
Z. Iqbal Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India

Search for other papers by Z. Iqbal in
Current site
Google Scholar
PubMed Close
,
M. Singh Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India

Search for other papers by M. Singh in
Current site
Google Scholar
PubMed Close
,
Y. T. Kamal Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India

Search for other papers by Y. T. Kamal in
Current site
Google Scholar
PubMed Close
, and
S. Ahmad Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India

Search for other papers by S. Ahmad in
Current site
Google Scholar
PubMed Close
Pages:
391–400
Online Publication Date:
01 Jun 2014
Publication Date:
01 Jun 2014
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.26.2014.2.13
Keywords:
andrographolide; betulinic acid; high-performance liquid chromatography; high-performance thin-layer chromatography
Open access

Summary

Andrographolide and betulinic acid are the terpenoids having potential anti-cancer activity. The cytotoxicity activity of both the drugs was carried out separately and in combination on liver cancer HepG2 cell lines. High-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods were developed and validated for simultaneous estimation of these two terpenoids as per the International Conference on Harmonization (ICH) guidelines, which was applied for quantification in nanoformulation. The retention time by HPLC and retardation factor by HPTLC for andrographolide and betulinic acid were found to be 2.2 and 6.6 min, and 0.24 ± 0.01 and 0.66 ± 0.01, respectively. Both the methods were validated for accuracy, precision, repeatability, robustness, limit of detection (LOD), and limit of quantitation (LOQ). The content of andrographolide and betulinic acid in nanoformulation was found to be 96.0% and 98.0% by HPLC and 96.59% and 98.33% by HPTLC, respectively, of labelled claim.

  • [1]. A. Varma , H. Padh, and N. Shrivastava, eCAM, 1–9 (2011), Article ID 815390. doi: 10.1093/ecam/nep135.

  • [2]. S. Fulda 2008 Int. J. Mol. Sci. 9 1096.

  • [3]. ICH, Q2 (R1), Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonization, Geneva, 2005, http://www.ich.org/LOB/media/MEDIA417.pdf.

    • Search Google Scholar
    • Export Citation

  • [4]. J. Jaiswal S. K. Gupta J. Kreuter 2004 J. Control. Release 96 169.

  • [5]. R. Parveen S. Baboota J. Ali A. Ahuja S.S. Vasudev S. Ahmad 2011 Int. J. Pharm. 413 245.

  • [6]. S. Ahmad R. Ahmad R. Parveen M. Mujeeb M. Aquil 2008 Chromatographia 67 441.

  • [7]. M. Singh Y.T. Kamal R. Parveen S. Ahmad 2011 J. Planar Chromatogr. 24 172.

  • [8]. Y.T. Kamal M. Singh E.T. Tamboli R. Parveen S. Ahmad 2011 Acta Chromatogr. 23 157.

  • [9]. R. Parveen S. Baboota J. Ali A. Ahuja S. Ahmad 2010 Biomed. Chromatogr. 24 639.

  • Collapse
  • Expand
Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

Indexing and Abstracting Services:

  • Science Citation Index
  • SCOPUS
  • DOAJ
  • Sci Search
  • Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences
  • GoogleScholar
  • Index Copernicus
  • CABI
  • CABELLS Journalytics

2024  
Scopus  
CiteScore  
CiteScore rank  
SNIP  
Scimago  
SJR index 0.285
SJR Q rank Q3

2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
Scopus  
CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
Scimago  
SJR index 0.344
SJR Q rank Q3

Acta Chromatographica
Publication Model Online only
Gold Open Access
Submission Fee none
Article Processing Charge

700 EUR/article

For MS submitted before 1 Feb 2025: 400 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Dec 2024 0 93 10
Jan 2025 0 50 6
Feb 2025 0 51 3
Mar 2025 0 50 3
Apr 2025 0 27 10
May 2025 0 20 3
Jun 2025 0 21 0