View More View Less
  • 1 Department of Pharmacognosy, ISF College of Pharmacy, Moga, 142001, India
Full access

Summary

A simple, selective, precise, and reproducible high-performance thin-layer chromatography (HPTLC) method for the analysis of vicine in herbal extracts and formulations containing Momordica charantia was developed. The study was performed on aluminum-based pre-coated TLC plates (silica gel 60F254). The chromatograms of samples were developed in twin trough glass chamber pre-saturated with mobile phase comprised of ethyl acetate-methanol-water-formic acid (7.5:3:1:0.1, v/v/v/v) at room temperature (25 ± 2°C). The densitometric analysis was carried out in absorbance mode at 278 nm. The optimized mobile phase showed a compact spot of vicine (RF = 0.31 ± 0.02) from the other constituents present in the samples. The linear regression analysis data for the calibration plots showed good linearity (r2 = 0.9990) with respect to peak area in the range of 200–1400 ng spot−1. The method was validated as per International Conference on Harmonization (ICH) guidelines. The limits of detection and quantification (50 and 150 ng spot−1, respectively) were also established. The proposed method has shown the excellent recovery (98.01–99.33%), which supports the suitability of the method for the analysis. Statistical analysis of the obtained data showed the selectivity of the proposed method for vicine estimation in the herbal samples.

  • [1]. J. K. Grover S. P. Yadav 2004 J. Ethnopharmacol. 93 123.

  • [2]. J. Virdi S. Sivakami S. Shahani A.C. Suthar M.M. Banavalikar M.K. Biyani 2003 J. Ethnopharmacol. 88 107.

  • [3]. M. Tan J. Ye N. Turner C. Hohnen-Behrens C. Ke C. Tang T. Chen H. Weiss E. Gesing A. Rowland D. E. James Y. Ye 2008 Chem. Biol. 15 263.

    • Search Google Scholar
    • Export Citation
  • [4]. S. Omar Z. Ansari M. Nehal 2007 Fitoterapia 78 46.

  • [5]. J. E. Cunnick K. Sakamoto S. K. Chapes G. W. Fortner D. J. Takemoto 1990 Cell Immunol. 126 278.

  • [6]. J. H. Kim E. M. Ju D. K. Lee H. J. Hwang 2002 Anticancer Res. 22 1885.

  • [7]. Y. Hwang J. W. Lee E. Hahm K. C. Jung J. H. Lee C. H. Park H. S. Rhee J. M. Ryu H. Kim C. Yang 2005 Biochem. Biophys. Res. Commun. 337 815.

    • Search Google Scholar
    • Export Citation
  • [8]. I. Ahmed M. S. Lakhani M. Gillett A. John H. Raza 2001 Diabetes Res. Clin. Pract. 51 155.

  • [9]. P. Chaturvedi 2005 Br. J. Biomed. Sci. 62 124.

  • [10]. P. Nerurkar Y. K. Lee E. H. Linden S. Lim L. Pearson J. Frank V. R. Nerurkar 2006 Br. J. Pharmacol. 148 1156.

  • [11]. N. M. Ansari L. Houlihan B. Hussain A. Pieroni 2005 Phytother. Res. 19 907.

  • [12]. I. Gurbuz C. Akyuz E. Yesilada B. Sener 2000 J. Ethnopharmacol. 7 77.

  • [13]. A. R. Biswas S. Ramaswamy J. S. Bapna 1991 J. Ethnopharmacol. 31 115.

  • [14]. J. Choi K. T. Lee H. Jung H. S. Park H. J. Park 2002 Arch. Pharm. Res. 25 336.

  • [15]. A. Sharanabasappa B. V. Saraswati B. Patil 2002 Pharm. Biol. 40 501.

  • [16]. V. P. Dixit P. Khanna S. K. Bhargava 1978 Planta Med. 34 280.

  • [17]. A. Raman C. Lau 1996 Phytomedicine 2 349.

  • [18]. S. I. Kim E. Hoehn N. A. M. Eskin F. Ismail 1982 J. Agric. Food Chem. 30 144.

  • [19]. H. S. Olsen J. H. Andersen 1978 J. Sci. Food Agric. 29 323.

  • [20]. H. Zhang Y. Wang X. Zhang M. Liu Z. Hu 2003 Anal. Lett. 36 1597.

  • [21]. E. A. Abourashed J. S. Mossa 2004 J. Pharm. Biomed. Anal. 36 617.

  • [22]. H. Agarawal N. Kaul A. R. Paradkar K. R. Mahadik 2004 J. Pharm. Biomed. Anal. 36 33.

  • [23]. V.B. Patravale S. D'souza Y. Narkar 2001 J. Pharm. Biomed. Anal. 25 685.

  • [24]. International Conference on Harmonization, Q2A, Geneva, 2005.

The author instruction is available in PDF.
Please, download the file from HERE.
 
The Open Access statement together with the description of the Copyright and License Policy are available in PDF.
Please, download the file from HERE.
 

 

Senior editors

Editor(s)-in-Chief: Kowalska, Teresa

Editor(s)-in-Chief: Sajewicz, Mieczyslaw

Editors(s) Danica Agbaba (University of Belgrade, Belgrade, Serbia);
Ivana Stanimirova-Daszykowska (University of Silesia, Katowice, Poland),
Monika Waksmundzka-Hajnos (Medical University of Lublin, Lublin, Poland)

Editorial Board

R. Bhushan (The Indian Institute of Technology, Roorkee, India)
J. Bojarski (Jagiellonian University, Kraków, Poland)
B. Chankvetadze (State University of Tbilisi, Tbilisi, Georgia)
M. Daszykowski (University of Silesia, Katowice, Poland)
T.H. Dzido (Medical University of Lublin, Lublin, Poland)
A. Felinger (University of Pécs, Pécs, Hungary)
K. Glowniak (Medical University of Lublin, Lublin, Poland)
B. Glód (Siedlce University of Natural Sciences and Humanities, Siedlce, Poland)
K. Kaczmarski (Rzeszow University of Technology, Rzeszów, Poland)
H. Kalász (Semmelweis University, Budapest, Hungary)
I. Klebovich (Semmelweis University, Budapest, Hungary)
A. Koch (Private Pharmacy, Hamburg, Germany)
Ł. Komsta (Medical University of Lublin, Lublin, Poland)
P. Kus (Univerity of Silesia, Katowice, Poland)
D. Mangelings (Free University of Brussels, Brussels, Belgium)
E. Mincsovics (Corvinus University of Budapest, Budapest, Hungary)
G. Morlock (Giessen University, Giessen, Germany)
J. Sherma (Lafayette College, Easton, PA, USA)
R. Skibiński (Medical University of Lublin, Lublin, Poland)
B. Spangenberg (Offenburg University of Applied Sciences, Germany)
T. Tuzimski (Medical University of Lublin, Lublin, Poland)
Y. Vander Heyden (Free University of Brussels, Brussels, Belgium)
A. Voelkel (Poznań University of Technology, Poznań, Poland)
B. Walczak (University of Silesia, Katowice, Poland)
W. Wasiak (Adam Mickiewicz University, Poznań, Poland)

KOWALSKA, TERESA
E-mail: kowalska@us.edu.pl

SAJEWICZ, MIECZYSLAW
E-mail:msajewic@us.edu.pl

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search
  • Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences
  • SCOPUS
  • GoogleScholar
  • Index Copernicus
  • CABI
2020
 
Total Cites
650
WoS
Journal
Impact Factor
1,639
Rank by
Chemistry, Analytical 71/83 (Q4)
Impact Factor
 
Impact Factor
1,412
without
Journal Self Cites
5 Year
1,301
Impact Factor
Journal
0,34
Citation Indicator
 
Rank by Journal
Chemistry, Analytical 75/93 (Q4)
Citation Indicator
 
Citable
45
Items
Total
43
Articles
Total
2
Reviews
Scimago
28
H-index
Scimago
0,316
Journal Rank
Scimago
Chemistry (miscellaneous) Q3
Quartile Score
 
Scopus
393/181=2,2
Scite Score
 
Scopus
General Chemistry 215/398 (Q3)
Scite Score Rank
 
Scopus
0,560
SNIP
 
Days from
58
sumbission
 
to acceptance
 
Days from
68
acceptance
 
to publication
 
Acceptance
51%
Rate

2019  
Total Cites
WoS
495
Impact Factor 1,418
Impact Factor
without
Journal Self Cites
1,374
5 Year
Impact Factor
0,936
Immediacy
Index
0,460
Citable
Items
50
Total
Articles
50
Total
Reviews
0
Cited
Half-Life
6,2
Citing
Half-Life
8,3
Eigenfactor
Score
0,00048
Article Influence
Score
0,164
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,05895
Average
IF
Percentile
20,349
Scimago
H-index
26
Scimago
Journal Rank
0,255
Scopus
Scite Score
226/167=1,4
Scopus
Scite Score Rank
Chemistry (miscellaneous) 240/398 (Q3)
Scopus
SNIP
0,494
Acceptance
Rate
41%

 

Acta Chromatographica
Publication Model Online only
Gold Open Access
Submission Fee none
Article Processing Charge 400 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

Acta Chromatographica
Language English
Size A4
Year of
Foundation
1992
Publication
Programme
2021 Volume 33
Volumes
per Year
1
Issues
per Year
4
Founder Institute of Chemistry, University of Silesia
Founder's
Address
PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 2083-5736 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Apr 2021 3 0 0
May 2021 4 0 0
Jun 2021 0 0 0
Jul 2021 0 1 0
Aug 2021 0 5 1
Sep 2021 0 2 2
Oct 2021 0 0 0