Authors:
A. Kwiecień Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland

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J. Krzek Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland

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M. Gądek Jagiellonian University Medical College, Faculty of Pharmacy Department of Inorganic and Analytical Chemistry 9 Medyczna Street 30-688 Kraków Poland

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The purpose of this work was to develop a TLC-densitometric method for simultaneous identification and quantitative determination of azithromycin, clarithromycin, roxithromycin, spiramycin, and troleandomycin. The method was developed on TLC aluminium plates precoated with silica gel F254 using solvent system izopropanol:n-hexane:ammonia 25% (8:12:3, v/v/v), which gives compact spots for azithromycin (RF = 0.65), clarithromycin (RF = 0.54), roxithromycin (RF = 0.49), spiramycin (RF = 0.22), and troleandomycin (RF = 0.36). Densitometric analysis was carried out at 478 nm after spraying with (1:4, v/v) sulphuric acid:ethanol and heating at 100°C for 5 min. The linear regression analysis data for the calibration plots showed good linear relationship with correlation coefficient higher than 0.99. The method is distinguished by high sensitivity, with limit of detection (LOD) from 0.34 μg/spot for troleandomycin to 0.67 μg/spot for clarithromycin and limit of quantification (LOQ) from 1.02 μg/spot for troleandomycin to 2.04 μg/spot for clarithromycin and a wide linearity range from 2 to 12 μg/spot for spiramycin and 2–15 μg/spot for other antibiotics. The precision of the determination was good; relative standard deviation (RSD) varied in the range from 1.49% to 4.14%.

  • A. Zejc and M. Gorczyca, Chemia leków (Drug chemistry), PZWL, Warszawa, Poland, (2004)

    Gorczyca M , '', in Chemia leków (Drug chemistry) , (2004 ) -.

  • European Pharmacopoeia, 4th ed., Council of Europe, Strasburg (2002)

  • British Pharmacopoeia, Her Majesty’s Stationery Office, London (2000)

  • U.S. Pharmacopoeia 24th Rev. U.S. Pharmacopeial Convention, Rockville, MD (1999)

  • M. J. Gonzalez de la Huebra and U. Vincent, J. Pharm. Biomed. Anal., 39, 376 (2005)

    Vincent U , '' (2005 ) 39 J. Pharm. Biomed. Anal. : 376 -.

  • I. Kanfer, M. Skinner, and R. Walker, J. Chromatogr. A, 812, 255 (1998)

    Walker R , '' (1998 ) 812 J. Chromatogr. A : 255 -.

  • J. Wang, Mass Spectrom. Rev., 28, 50 (2009)

    Wang J , '' (2009 ) 28 Mass Spectrom. Rev. : 50 -.

  • P. Khashaba, J. Pharm. Biomed. Anal., 27, 923 (2002)

    Khashaba P , '' (2002 ) 27 J. Pharm. Biomed. Anal. : 923 -.

  • N. El-Rabbat, H. F. Askal, P. Y. Khashaba, and N. N. Attia, J. AOAC Int., 89, 1276 (2006)

    Attia N N , '' (2006 ) 89 J. AOAC Int. : 1276 -.

  • C. M. Bhaskar Reddy and G. V. Subbareddy, J. Chem. Pharm. Res., 4, 3684 (2012)

    Subbareddy G V , '' (2012 ) 4 J. Chem. Pharm. Res. : 3684 -.

  • S. Ashour and R. Bayram, Spectrochim. Acta Part A Mol. Biomol. Spectrosc., 99, 74 (2012)

    Bayram R , '' (2012 ) 99 Spectrochim. Acta Part A Mol. Biomol. Spectrosc. : 74 -.

  • M. Walash, M. Rizk, M. Eid, and M. Fathy, J. AOAC Int., 90, 1579 (2007)

    Fathy M , '' (2007 ) 90 J. AOAC Int. : 1579 -.

  • Y. M. Liu, Y. Yang, L. F. Peng, and L. Mei, Curr. Anal. Chem., 7, 325 (2011)

    Mei L , '' (2011 ) 7 Curr. Anal. Chem. : 325 -.

  • Z. Wang, Z. Yang, J. Ye, G. Tan, H. Xu, and Y. Liu, Chem. Anal. (Warsaw), 54, 883 (2009)

    Liu Y , '' (2009 ) 54 Chem. Anal. (Warsaw) : 883 -.

  • L. Zhang, X. Duan, Y. Wen, J. Xu, Y. Yao, Y. Lu, L. Lu, and O. Zhang, Electrochim. Acta, 2, 179 (2012)

    Zhang O , '' (2012 ) 2 Electrochim. Acta : 179 -.

  • J. Nowakowska, J. Planar Chromatogr., 18, 455 (2005)

    Nowakowska J , '' (2005 ) 18 J. Planar Chromatogr. : 455 -.

  • J. Nowakowska, J. Planar Chromatogr., 19, 62 (2006)

    Nowakowska J , '' (2006 ) 19 J. Planar Chromatogr. : 62 -.

  • J. Nowakowska, J. Planar Chromatogr., 17, 200 (2004)

    Nowakowska J , '' (2004 ) 17 J. Planar Chromatogr. : 200 -.

  • P. Loya and P. Hamrapurkar, J. Planar Chromatogr., 24, 534 (2011)

    Hamrapurkar P , '' (2011 ) 24 J. Planar Chromatogr. : 534 -.

  • T. Tosti, K. Drljević, D. Milojković-Opsenica, and Ž. Tešić, J. Planar Chromatogr., 18, 415 (2005)

    Tešić , '' (2005 ) 18 J. Planar Chromatogr. : 415 -.

  • U. Vincent, G. Gizzi, C. von Holst, J. De Jong, and J. Michard, Food Addit. Contam., 24, 351 (2007)

    Michard J , '' (2007 ) 24 Food Addit. Contam. : 351 -.

  • H. M. Maher and R. M. Youssef, Chromatographia, 69, 345 (2009)

    Youssef R M , '' (2009 ) 69 Chromatographia : 345 -.

  • L. Yu, J. Q. Wang, G. L. Wang, Q. Y. Pang, and Y. Lu, Chin. J. Antibiot., 26, 227 (2001)

    Lu Y , '' (2001 ) 26 Chin. J. Antibiot. : 227 -.

  • A. Kwiecień, J. Krzek, and Ł. Biniek, J. Planar Chromatogr. Mod. TLC, 21, 177 (2008)

    Biniek , '' (2008 ) 21 J. Planar Chromatogr. Mod. TLC : 177 -.

  • A. Kwiecień, J. Krzek, and M. Walczak, J. AOAC Int., 95, 1418 (2012)

    Walczak M , '' (2012 ) 95 J. AOAC Int. : 1418 -.

  • J. Nowakowska, J. Halkiewicz, and J. W. Łukasiak, J. Planar Chromatogr., 14, 350 (2001)

    Łukasiak J W , '' (2001 ) 14 J. Planar Chromatogr. : 350 -.

  • J. Nowakowska, J. Halkiewicz, and J. W. Łukasiak, Chromatographia, 56, 367 (2002)

    Łukasiak J W , '' (2002 ) 56 Chromatographia : 367 -.

  • C. Q. Hu, and W. B. Zou, W. S. Hu, X. K. Ma, M. Z. Yang, S. L. Zhou, J. F. Sheng, S. H. Cheng, and J. Xue, J. Pharm. Biomed. Anal., 40, 68 (2006)

    Xue J , '' (2006 ) 40 J. Pharm. Biomed. Anal. : 68 -.

  • J. Krzek, A. Kwiecień, M. Starek, A. Kierszniewska, and W. Rzeszutko, J. AOAC Int., 83, 1502 (2000)

    Rzeszutko W , '' (2000 ) 83 J. AOAC Int. : 1502 -.

  • J. Krzek, M. Starek, A. Kwiecień, and W. Rzeszutko, J. Pharm. Biomed. Anal., 24, 629 (2001)

    Rzeszutko W , '' (2001 ) 24 J. Pharm. Biomed. Anal. : 629 -.

  • International Conference on Harmonization. Validation of Analytical Procedures: Text and Methodology, ICH-Q2 (R1), Geneva, Switzerland (2005), http://www.ich.org/LOB/media/MEDIA417.pdf

  • A. Khedr and M. Sheha, J. Chrom. Sci., 41, 10 (2003)

    Sheha M , '' (2003 ) 41 J. Chrom. Sci. : 10 -.

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Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

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2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
Scopus  
CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
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SJR index 0.344
SJR Q rank Q3

Acta Chromatographica
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Acta Chromatographica
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
4
Founder Institute of Chemistry, University of Silesia
Founder's
Address
PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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