Taxodione is the abietane diterpenoid, which has been earlier reported to show cytotoxic, antitumor, antibacterial, and antioxidant properties. This compound has been isolated from a genetically transformed root culture of Salvia austriaca. However, no method for taxodione quantification is yet available. A validated ultra-performance liquid chromatography-diode array detector (UPLC-DAD) method was developed for the quantification of taxodione in a hexane extract taken from genetically transformed roots of S. austriaca. The developed UPLC-DAD method demonstrated good linearity in the range 10–500 μg mL−1 (r2 = 0.99988), high precision (relative standard deviation (RSD) <1.42%), and good repeatability (RSD <1.84%). The limits of detection and quantification of taxodione were found to be 0.48 ng and 1.60 ng, respectively. The recovery of taxodione ranged between 90.4 and 113.1%, and the RSD was less than 2.5%.The developed UPLC-DAD method was then used to determine the content of taxodione in various hairy root lines and changes in amount of the compound during the 50-day growth cycle of the culture. The content of taxodione in hairy root cultures of S. austriaca ranged from 0.29 to 1.12 mg g−1 dry weight depending on root line and stage of growth.
J. Bernáth and E. Németh. In: S.E. Kintzios (Ed) Sage. The genus Salvia, Harwood Academic Publishers, The Netherlands, 2000, pp. 111–112
A. Ulubelen, Phytochemistry, 64, 395 (2003)
Ulubelen A , '' (2003 ) 64 Phytochemistry : 395 -.
S.M. Kupchan, A. Karim, and C. Marcks, J. Org. Chem., 34, 3912 (1969)
Marcks C , '' (1969 ) 34 J. Org. Chem. : 3912 -.
L. Moujir and A.M. Gutierrez-Navarro, Phytother. Res., 10, 172 (1996)
Gutierrez-Navarro AM , '' (1996 ) 10 Phytother. Res. : 172 -.
Z. Yang, Y. Kitano, K. Chiba, N. Shibata, H. Kurokawa, Y. Doi, Y. Arakawa, and M. Tada, Bioorg. Med. Chem., 9, 347 (2001)
Tada M , '' (2001 ) 9 Bioorg. Med. Chem. : 347 -.
Ł. Kuźma, W. Kisiel, A. Królicka, and H. Wysokińska, Pharmazie, 66, 904 (2011)
Wysokińska H , '' (2011 ) 66 Pharmazie : 904 -.
O.L. Gamborg, R. Miller, and K. Ojima, Exp. Cell Res., 50, 151 (1968)
Ojima K , '' (1968 ) 50 Exp. Cell Res. : 151 -.
Y. Zhou, G. Xu, F.F.K. Choi, L. Ding, Q.B. Han, J.Z. Song, C.F. Qiao, Q. Zhao, and H. Xu, J. Chromatogr., A, 1216, 4847 (2009)
Xu H , '' (2009 ) 1216 J. Chromatogr., A : 4847 -.