Ł. Kuźma Medical University of Łódź Department of Biology and Pharmaceutical Botany Muszyńskiego 1 st. 90-151 Łódź Poland

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H. Wysokińska Medical University of Łódź Department of Biology and Pharmaceutical Botany Muszyńskiego 1 st. 90-151 Łódź Poland

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Taxodione is the abietane diterpenoid, which has been earlier reported to show cytotoxic, antitumor, antibacterial, and antioxidant properties. This compound has been isolated from a genetically transformed root culture of Salvia austriaca. However, no method for taxodione quantification is yet available. A validated ultra-performance liquid chromatography-diode array detector (UPLC-DAD) method was developed for the quantification of taxodione in a hexane extract taken from genetically transformed roots of S. austriaca. The developed UPLC-DAD method demonstrated good linearity in the range 10–500 μg mL−1 (r2 = 0.99988), high precision (relative standard deviation (RSD) <1.42%), and good repeatability (RSD <1.84%). The limits of detection and quantification of taxodione were found to be 0.48 ng and 1.60 ng, respectively. The recovery of taxodione ranged between 90.4 and 113.1%, and the RSD was less than 2.5%.The developed UPLC-DAD method was then used to determine the content of taxodione in various hairy root lines and changes in amount of the compound during the 50-day growth cycle of the culture. The content of taxodione in hairy root cultures of S. austriaca ranged from 0.29 to 1.12 mg g−1 dry weight depending on root line and stage of growth.

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Senior editors

Editor(s)-in-Chief: Kowalska, Teresa (1946-2023)

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland


  • Danica Agbaba, University of Belgrade, Belgrade, Serbia
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Yvan Vander Heyden, Free University of Brussels, Brussels, Belgium
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Brata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation


KOWALSKA, TERESA (1946-2023)


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Acta Chromatographica
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ISSN 2083-5736 (Online)

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