Acetyl salicylic acid (ASA), as the major drug consumed in the world, has been combined with other various drugs. In this regard, ASA-rosuvastatin is the most recent combination. The current article reported the first two separation methods for the assay of that combination utilizing sequential injection chromatography (SIC) and high-performance liquid chromatography (HPLC). The optimization process was conducted for SIC, and the optimum conditions were then adapted to HPLC. Both methods, SIC and HPLC, were validated and successfully applied to tablet formulation. In both methods, the analytes were chromatographed onto a short C18 monolithic column (4.6 × 25 mm) by a mobile phase composition of 10 mmol L−1 phosphate:acetonitrile: methanol (50:30:20, v/v/v at pH 3.0). The performances of both methods were compared. The remarkable advantages of the SIC method over HPLC method were the reduction in reagent consumption and instrumentation simplicity and cost-effectiveness. The total volume of mobile phase consumed is three orders of magnitude larger in HPLC than that in SIC. Other analytical features of the SIC and HPLC methods were comparable and acceptable for pharmaceutical analysis. Accordingly, the SIC method could be more suitable for routine analysis of simple matrices after examining the capability of the technique to work under industrial conditions.
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