Acta Chromatographica
Volume/Issue:
Volume 27: Issue 2
Development and validation of simple, rapid and sensitive LC-PDA ultraviolet method for quantification of Nebivolol in rat plasma and its application to pharmacokinetic studies
Authors:
P. Ravi BITS-Pilani Hyderabad Campus Pharmacy Department Jawaharnagar, Ranga Reddy (Dist.) Andhra Pradesh India

Search for other papers by P. Ravi in
Current site
Google Scholar
PubMed Close
,
R. Vats BITS-Pilani Hyderabad Campus Pharmacy Department Jawaharnagar, Ranga Reddy (Dist.) Andhra Pradesh India

Search for other papers by R. Vats in
Current site
Google Scholar
PubMed Close
,
S. Joseph BITS-Pilani Hyderabad Campus Pharmacy Department Jawaharnagar, Ranga Reddy (Dist.) Andhra Pradesh India

Search for other papers by S. Joseph in
Current site
Google Scholar
PubMed Close
, and
N. Gadekar BITS-Pilani Hyderabad Campus Pharmacy Department Jawaharnagar, Ranga Reddy (Dist.) Andhra Pradesh India

Search for other papers by N. Gadekar in
Current site
Google Scholar
PubMed Close
Pages:
281–294
Online Publication Date:
11 Apr 2014
Publication Date:
01 Jun 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/achrom.27.2015.2.6
Keywords:
liquid chromatography; nebivolol; internal standard; pharmacokinetic studies
Open access

A simple, sensitive and rapid high-performance liquid chromatography method with ultraviolet (UV) detector was developed and validated for the analysis of Nebivolol (NBL) in rat plasma. The plasma sample, spiked with raloxifene hydrochloride as an internal standard (IS), was subjected to single step protein precipitation method prior to analysis. Chromatographic separation was achieved on the Agilent C8 (150 mm × 4.6 mm, 5 μm) column and monitored at a wavelength of 280 nm. Elution was carried out, in an isocratic mode, using a mobile phase consisting of acetonitrile and potassium di-hydrogen orthophosphate buffer (pH 3.5 ± 0.1) in the ratio of 37:63 v/v. Retention times of IS and NBL were 5.1 ± 0.10 min and 8.01 ± 0.12 min, respectively. No interference was observed from plasma components in the analysis of NBL and IS. Calibration curve was linear over the range of 125–3000 ng/mL (r2 = 0.999). NBL was found to be stable under various processing and storage conditions. The developed method was applied in the quantification of NBL in plasma samples, determining various pharmacokinetic parameters from intravenous bolus and oral administration of the drug in Wistar rats. NBL was found to follow two compartmental open models in Wistar rats.

  • A. Van de Water, W. Janssens, J. Van Neuten, R. Xhonneux, J. De Cree, H. Verhaegen, R.S. Reneman, P.A. Janssen, J. Cardiovasc. Pharmacol., 11, 552 (1988)

    Janssen P.A. , '' (1988 ) 11 J. Cardiovasc. Pharmacol. : 552 -.

  • J.R. Cockcroft, P.J. Chowienczyk, S.E. Brett, C.P. Chen, A.G. Dupont, L. Van Nueten, S.J. Wooding, J.M. Ritter, J. Pharmacol. Exp. Therapeut., 274, 1067 (1995)

    Ritter J.M. , '' (1995 ) 274 J. Pharmacol. Exp. Therapeut. : 1067 -.

  • D. Wojciechowski, V. Papademetriou, Expert Rev. Cardiovasc. Ther., 6, 471 (2008)

    Papademetriou V. , '' (2008 ) 6 Expert Rev. Cardiovasc. Ther. : 471 -.

  • L. Van Nueten, J. De Crée, Cardiovasc. Drugs Ther., 12, 339 (1998)

    Crée J. , '' (1998 ) 12 Cardiovasc. Drugs Ther. : 339 -.

  • W. McNeely, K.L. Goa, Drugs, 57, 633 (1999)

    Goa K.L. , '' (1999 ) 57 Drugs : 633 -.

  • M.A. Weber, Am. J. Hypertens., 18, 169S (2005)

    Weber M.A. , '' (2005 ) 18 Am. J. Hypertens. : 169S -.

  • T. Mizutani, Drug Metab. Rev., 35, 99 (2003)

    Mizutani T. , '' (2003 ) 35 Drug Metab. Rev. : 99 -.

  • W.E. Evans, M.V. Relling, A. Rahman, H.L. McLeod, E.P. Scott, J.S. Lin, J. Clin. Invest., 91, 2150 (1993)

    Lin J.S. , '' (1993 ) 91 J. Clin. Invest. : 2150 -.

  • R. Weiss, Vasc Health Risk Manag., 2, 303 (2006)

    Weiss R. , '' (2006 ) 2 Vasc Health Risk Manag. : 303 -.

  • B.S. Nayak, P. Ellaiah, D. Pattanayak, S. Das, Asian J. Pharmaceut., 5, 175 (2011)

    Das S. , '' (2011 ) 5 Asian J. Pharmaceut. : 175 -.

  • S.S. Bushetti, P.M. Prashant, S. Kardame, RGUHS J. Pharmaceut. Sci., 1, 157 (2011)

    Kardame S. , '' (2011 ) 1 RGUHS J. Pharmaceut. Sci. : 157 -.

  • F.M. Bertera, J.S. Del Mauro, A.H. Polizio, D. Chiappetta, C.A. Taira, C. Höcht, Naunyn-Schmiedeberg’s Arch. Pharmacol., 385, 833 (2012)

    Höcht C. , '' (2012 ) 385 Naunyn-Schmiedeberg’s Arch. Pharmacol. : 833 -.

  • L. Ma, R. Gul, J. Habibi, M. Yang, L. Pulakat, A. Whaley-Connell, C.M. Ferrario, J.R. Sowers, Am. J. Physiol. Heart Circ. Physiol., 302, H2341 (2012)

    Sowers J.R. , '' (2012 ) 302 Am. J. Physiol. Heart Circ. Physiol. : H2341 -.

  • G. Sacco, S. Evangelista, S. Manzini, M. Parlani, M. Bigioni, Fut. Cardiol., 7, 757 (2011)

    Bigioni M. , '' (2011 ) 7 Fut. Cardiol. : 757 -.

  • D.A. Shah, K.K. Bhatt, R.S. Mehta, S.L. Baldania, T.R. Gandhi, Indian J. Pharm. Sci., 70, 591 (2008)

    Gandhi T.R. , '' (2008 ) 70 Indian J. Pharm. Sci. : 591 -.

  • S.U. Kokil, M.S. Bhatia, Indian J. Pharm. Sci., 71, 111 (2009)

    Bhatia M.S. , '' (2009 ) 71 Indian J. Pharm. Sci. : 111 -.

  • S.N. Meyyanathan, A.S. Birajdar, B. Suresh, Indian J. Pharm. Edu. Res., 44, 156 (2010)

    Suresh B. , '' (2010 ) 44 Indian J. Pharm. Edu. Res. : 156 -.

  • L. Abdel-Fattah, L. Abdel-Aziz, A. El-Kosasy, M. Gaied, Drug Discov. Ther., 4, 418 (2010)

    Gaied M. , '' (2010 ) 4 Drug Discov. Ther. : 418 -.

  • N.V. Ramakrishna, K.N. Vishwottam, M. Koteshwara, S. Manoj, M. Santosh, D.P. Varma, J. Pharm. Biomed. Anal., 39, 1006 (2005)

    Varma D.P. , '' (2005 ) 39 J. Pharm. Biomed. Anal. : 1006 -.

  • J. Nandania, S.J. Rajput, P. Contractor, P. Vasava, B. Solanki, M. Vohra, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 923–924, 110–119 (2013)

    Chromatogr J. , '' (2013 ) 923–924 B Analyt. Technol. Biomed. Life Sci. : 110 -119 .

  • P.S. Selvan, K.V. Gowda, U. Mandal, W.D. Solomon, T.K. Pal, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 858, 143 (2007)

    Pal T.K. , '' (2007 ) 858 J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. : 143 -.

  • International Conference on Harmonization (ICH), Harmonized tripartite guideline on Topic Q2B, Notes for Guidelines for Validation of Analytical Procedures: Methodology; Pub. By: The European Agency for the Evaluation of Medicinal Products, Human Medicines Evaluation Unit (1996)

  • Guidance for Industry: Bioanalytical method validation. U.S. Department of Health and Human Services, Food and Drug Administration (2001)

  • D.V. McCalley, J. Chromatogr. A, 708, 185 (1995)

    McCalley D.V. , '' (1995 ) 708 J. Chromatogr. A : 185 -.

  • C. Stella, S. Rudaz, M. Mottaz, P.A. Carrupt, J.L. Veuthey, J. Sep. Sci., 27, 284 (2004)

    Veuthey J.L. , '' (2004 ) 27 J. Sep. Sci. : 284 -.
  • Collapse
  • Expand
Cover Acta Chromatographica
Acta Chromatographica
Online ISSN:
2083-5736

Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

Indexing and Abstracting Services:

  • Science Citation Index
  • SCOPUS
  • DOAJ
  • Sci Search
  • Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences
  • GoogleScholar
  • Index Copernicus
  • CABI
  • CABELLS Journalytics

2024  
Scopus  
CiteScore  
CiteScore rank  
SNIP  
Scimago  
SJR index 0.285
SJR Q rank Q3

2023  
Web of Science  
Journal Impact Factor 1.7
Rank by Impact Factor Q3 (Chemistry, Analytical)
Journal Citation Indicator 0.43
Scopus  
CiteScore 4.0
CiteScore rank Q2 (General Chemistry)
SNIP 0.706
Scimago  
SJR index 0.344
SJR Q rank Q3

Acta Chromatographica
Publication Model Online only
Gold Open Access
Submission Fee none
Article Processing Charge 700 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

Acta Chromatographica
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 4
Founder Institute of Chemistry, University of Silesia
Founder's
Address		 PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads