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  • 1 Sun Yat-sen University, No. 132 East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China
  • 2 Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China

A high-speed counter-current chromatography (HSCCC) method was established for the isolation and purification of isochlorogenic acid A from Lonicera japonica Thunb. The two-phase solvent system was composed of n-hexane:ethyl acetate: isopropanol:water (2:3:2:5, v/v/v/v). From 150 mg of the ethyl acetate fraction of L. japonica Thunb, 19.65 mg of isochlorogenic acid A was obtained in a one-step HSCCC separation, with a purity of 99.1%, as determined by high-performance liquid chromatography (HPLC). The structure was further identified by ultraviolet (UV), mass spectrometry (MS) and nuclear magnetic resonance (NMR).

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    China Pharmacopoeia Committee, Pharmacopoeia of the People's Republic of China, First Division (2010 edition), China Chemical Industry Press, Beijing, China, 2009, p. 205

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