View More View Less
  • 1 Medical University of Lublin, Chodźki 4A St., 20-093 Lublin, Poland
  • | 2 Botanical Garden of Maria Curie-Skłodowska University in Lublin, Sławinkowska 3, 20-810 Lublin, Poland
Open access

Micro-thin-layer chromatography in two dimensional (2D-mTLC) mode in normal and reversed phase systems by use of diol bonded stationary phase was applied to make fingerprints of 11 species of Mentha genus and two finished pharmaceutical products.

Nonaqueous eluents (propan-2-ol or ethyl acetate dissolved in n-heptane) were used in normal phase systems. Mixtures of acetonitrile with water were used in reversed phase chromatographic systems.

Optimization of one dimensional systems was performed by determining of RF vs. composition of mobile phases dependencies for standards occurring in various species of Mentha. Most selective eluents were chosen to optimize two-dimensional systems by creating RF in normal-phase (NP) systems vs. RF in reversed-phase (RP) systems correlations.

2D-mTLC on diol polar bonded stationary phase were optimized to separate phenolic compounds and make fingerprints of examined plant materials and this method was never applied earlier in the chromatographic analysis.

  • [1]

    D.N. Olennikov and L.M. Tankhaeva, Chem. Nat. Comp., 46 (1), 2327 (2010)

  • [2]

    B. Shan , Y.Z. Cai, M. Sun, and H. Corhe, J. Agric. Food Chem., 53, 77497759 (2005)

  • [3]

    M. Gulluce , F. Sahin, M. Sokmen, H. Ozer, D. Daferera, A. Sokmen, M. Polissiou, A. Adiguzel, and H. Ozkan, Food Chem., 103, 14491456 (2007)

    • Search Google Scholar
    • Export Citation
  • [4]

    M.S. Alia , M. Saleema, W. Ahmada, M. Parvezb, and R. Yamdagnib, Phytochemistry, 59, 889895 (2002)

  • [5]

    M.A. Hawryl , M.A. Niemiec, and M. Waksmundzka-Hajnos, J. Planar Chromatogr. 26, 141146 (2013)

  • [6]

    P.K. Zarzycki , J Chromatogr. A 1187, 250259 (2008)

  • [7]

    P.K. Zarzycki , M.M. Slaczka, E. Wlodarczyk, and M.J. Baran, Chromatographia 76, 12491259 (2013)

  • [8]

    M. B. Zarzycka , Camera Separatoria, 3, 129145 (2011)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jan 2021 0 5 9
Feb 2021 0 5 6
Mar 2021 0 4 10
Apr 2021 0 0 1
May 2021 0 5 12
Jun 2021 0 0 4
Jul 2021 0 0 0