View More View Less
  • 1 National Pingtung University of Science and Technology, Pintung 912, Taiwan
  • | 2 National Pingtung University of Science and Technology, Pingtung 912, Taiwan
  • | 3 National Pingtung University of Science and Technology, Pingtung 912, Taiwan
  • | 4 National Pingtung University of Science and Technology, Pingtung 912, Taiwan
  • | 5 Biotech Total Solutions Co., Ltd., New Taipei City 116, Taiwan
  • | 6 National Taitung University, Taitung 950, Taiwan
Open access

Cross Mark

An accurate and rapid liquid chromatography–electrospray ionizaion– tandem mass spectrometry (LC—ESI—MS/MS) analytical method was developed and validated for the simultaneous determination of antcins A, B, C, H, and K, dehydroeburicoic acid, and 4,7-dimethoxy-5-methyl-1,3-benzodioxole in the extract and capsule of Antrodia cinnamomea (AC) fruiting body. These seven signature compounds were ionized using an electrospray ion source and analyzed by a triple-quadrupole mass analyzer under a multiple reaction monitoring (MRM) mode. The MRM transitions of m/z 453/409 (antcin A), m/z 467/408 (antcin B), m/z 469/425 (antcin C), m/z 485/413 (antcin H), m/z 487/407 (antcin K), m/z 467/337 (dehydroeburicoic acid), and m/z 197/139 (4,7-dimethoxy-5-methyl-1,3-benzodioxole) were used to quantify these seven components, respectively. Their calibration curves presented good linear regressions (R2 > 0.997) within the tested concentration range. The intra- and inter-day precisions were less than 1.97% and 2.53%, respectively. The overall recovery was in the range of 87.55%–95.41%. This validated high-performance liquid chromatography (HPLC)—MS/MS method offers promising applications for the accurate and rapid quantification of signature compounds in the fruiting body and its commercial products.

  • [1]

    I. H. Cherng , H. C. Chang, M. C. Cheng, and Y. Wang, J. Nat. Prod., 58, 365 (1995)

  • [2]

    T. T. Chang and W. N. Chou, Bot. Bull. Acad. Sin., 45, 347355 (2004)

  • [3]

    Z. H. Ao , Z. H. Su, Z. Lu, H. Y. Xu, X. M. Zhang, and W. F. Dou, J. Ethnopharmacol., 121, 194 (2009)

  • [4]

    I. H. Cherng , D. P. Wu, and H. C. Chiang, Phytochmeistry, 41, 263 (1996)

  • [5]

    Y. C. Shen , C. F. Chen, Y. H. Wang, T. T. Chang, and C. J. Chou, Pharm. J., 55, 313 (2003)

  • [6]

    C. C. Shen , Y. H. Wang, T. T. Chang, L. C. Lin, M. J. Don, Y. C. Hou, K. T. Liou, S. Chang, W. Y. Wang, H. C. Ko, and Y. C. Shen, Planta. Med., 73, 1208 (2007)

    • Search Google Scholar
    • Export Citation
  • [7]

    C. H. Chen , S. W. Yang, and Y. C. Shem, J. Nat. Prod., 58, 1655 (1995)

  • [8]

    J. J. Chen , W. J. Lin, C. H. Liao, and P. C. Shieh, J. Nat. Prod., 70, 989 (2007)

  • [9]

    K. B. Male , Y. K. Rao, and Y. M. Tzeng, Chem. Res. Toxicol., 21, 2127 (2008)

  • [10]

    C. T. Yeh , Y. K. Rao, C. J. Yao, C. F. Yeh, C. H. Li, S. E. Chuang, J. H. Luong, G. M. Lai, and Y. M. Tzeng, Cancer. Lett., 285, 73 (2009)

  • [11]

    D. Steinmann and M. Ganzera, J. Pharm. Biomed. Anal., 55, 744 (2010)

  • [12]

    C. Ho , C. Lam, M. Chan, R. Cheung, L. Law, L. Liu, K. Ng, M. Suen, and H. Tai, Clin. Biochem. Rev., 24, 3 (2003)

  • [13]

    J. Xing , C. F. Xie, and H. X. Lou, J. Pharm. Biomed. Anal., 44, 368 (2007)

  • [14]

    M. Yang , J. Sun, Z. Lu, G. Chen, S. Guan, X. Liu, B. Jiang, M. Ye, and Da. Guo, J. Chromatogr. A, 1216, 2045 (2009)

  • [15]

    J. L. Zhou , L. W. Qi, and P. Li, J. Chromatogr. A, 1216, 7582 (2009)

  • [16]

    C. Y. Chang , M. Y. Lue, and T. M. Pan, J. Food. Drug. Anal., 13, 338 (2005)

  • [17]

    T. Y. Lin , C. Y. Chen, S. Y. Chien,, W. W. Hsiao, F. H. Chu, W. H. Li, C. C. Lin, J. F. Shaw, and S. Y. Wang, J. Agric. Food. Chem., 59, 7626 (2011)

    • Search Google Scholar
    • Export Citation
  • [18]

    S. H. Tu , C. H. Wu, J. C. Chen, C. S. Huang, H. W. Chang, C. H. Chang, H. M. Lien, and Y. S. Ho, J. Agric. Food. Chem., 60, 3612 (2012)

  • [19]

    H. C. Wang , F. H. Chu, S. C. Chien, J. W. Liao, H. W. Hsieh, W. H. Li, C. C. Lin, J. F. Shaw, Y. H. Kuo, and S. Y. Wang, J. Agric. Food. Chem., 61, 8556 (2013)

    • Search Google Scholar
    • Export Citation
  • [20]

    C. H. Huang , Y. Y. Chang, C. W. Liu, W. Y. Kang, Y. L. Lin, H. C. Chang, and Y. C. Chen, J. Agric. Food. Chem., 58, 3859 (2010)

  • [21]

    U. Justesen , J. Chromatogr. A, 902, 369 (2000)

  • [22]

    H. J. Zhang , P. Shen, and Y. Y. Cheng, J. Pharm. Biomed. Anal., 34, 705 (2004)

  • [23]

    Y. J. Hsieh , L. C. Lin, and T. H. Tsai, J. Chromatogr. A, 1083, 141 (2005)

  • [24]

    D. P. Singh , R. Govindarajan, and A. K. S. Rawat, Phytochem. Anal., 19, 164 (2008)

  • [25]

    A. Niranjan , J. Barthwal, A. Lehri, D. P. Singh, R. Govindrajan, A. K. S. Rawat, and D. V. Amla, Acta Chromatogr., 21, 105 (2009)

  • [26]

    Y. C. Du , T. Y. Wu, F. R. Chang, W. Y. Lin, Y. M. Hsu, F. T. Cheng, C. Y. Lu, M. H. Yen, Y. T. Tsui, H. L. Chen, M. F. Hou, M. C. Lu, and Y. C. Wu, J. Pharm. Biomed. Anal., 58, 182 (2012)

    • Search Google Scholar
    • Export Citation
  • [27]

    Y. Liu , X. Di, X. Liu, W. Shen, and K. S. Leung, J. Pharm. Biomed. Anal., 53, 781 (2010)

  • [28]

    X. G. He , J. Chromatogr. A, 880, 203 (2000)

  • [29]

    C. C. Shen , Y. C. Kuo, R. L. Huang, L. C. Lin, M. J. Don, T. T. Chang, and C. J. Chou, J. Chin. Med., 14, 247 (2003)

  • [30]

    H. C. Chiang , D. P. Wu, I. W. Cherng, and C. H. Ueng, Phytochemistry, 39, 613 (1995)

  • [31]

    S. S. Zhao and S. Y. Leung. Chin. Med., 5, 4 (2010)

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 44 36 0
PDF Downloads 61 56 3