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Camila C. Pires University of Caxias do Sul, RS, Brazil

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Moacir Kaiser Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

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Lauren D. Grünspan University of Caxias do Sul, Caxias do Sul, RS, Brazil

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Fabiano Barreto Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

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Adrine Innocente Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

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Simone Gnoatto Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

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João V. Laureano Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

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Bibiana V. Araujo Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

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Teresa Dalla Costa Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

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Leandro Tasso University of Caxias do Sul, RS, Brazil
University of Caxias do Sul, Caxias do Sul, RS, Brazil

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Open access

An accurate and reliable LC—MS/MS assay was firstly developed and validated for quantitative determination of a new antimalarial prototype drug, 3β-hydroxyurs-12-en-28-oic acid (LAFIS 01), in rat plasma. Dexamethasone was employed as internal standard. Simple protein precipitation by acetonitrile for the sample preparation was used. Effective separation was achieved with Phenomenex Luna C18 (50 × 2 mm, 5 μm) column. The mobile phase consisted of (A) water and (B) acetonitrile, both containing 0.1% acetic acid, delivered by gradient elution. The column temperature was maintained at 40 °C. The LAFIS 01 was monitored by electrospray ionization interface, operating in the negative mode (ESI−) in multiple reactions monitoring (MRM), checking the transitions 455 > 455 for LAFIS 01 and 451 > 361 for the IS. Once LAFIS 01 demonstrated low fragmentation by collision-induced dissociation (CID) nonpresenting abundant high-intensity fragments to meet the desired concentration levels quantification, only pseudomolecular ion was monitored. The flow rate was 500 μL min−1. The lower limit of quantitation achieved was 10 ng mL−1 and linearity was observed from 10 to 500 ng mL−1. The relative standard deviation (RSD) values of the intra- and inter-assay precisions of the method were below 8.42 and 7.94%, respectively. The accuracy ranged from 92.05 to 102.94%. The extraction recovery of LAFIS 01 and IS was up to 90%. The method showed linearity, precision, accuracy, sensitivity, and stability required to quantify LAFIS 01 in preclinical pharmacokinetic study.

  • [1]

    WHO, World Malaria Report, Geneva, 2013

  • [2]

    I. Petersen , R. Eastman, and M. Lanzer, FEBS Lett., 585, 15511562 (2011)

  • [3]

    T. Mita , K. Tanabe, and K. Kita, Parasitol Int., 5, 201209 (2009)

  • [4]

    Q. Cheng , D.E. Kyle, and M.L. Gatton, Int. J. Parasitol, 2, 249255 (2012)

  • [5]

    D.A. Fidock , P.J. Rosenthal, S.L. Croft, R. Brun, and S. Nwaka, Nature Rev., 3, 509520 (2001)

  • [6]

    J. Wiesner and H. Jomaa, Curr. Drug Targ., 8, 313 (2007)

  • [7]

    M.N. Aminake , S. Shoof, L. Sologub, M. Leubner, M. Kirschner, A. Hans-Dieter, and G. Pradel, Antimicrob. Agents Chemother., 55, 13381348 (2011)

    • Search Google Scholar
    • Export Citation
  • [8]

    S.C.B. Gnoatto , S. Susplugas, L. Dalla Vechia, T.B. Ferreira, A. Dassonville-Klimpt, K.R. Zimmer, C. Demailly, S. Nascimento, J. Guillon, P. Grellier, H. Verli, G. Gosmann, and P. Sonnet, Bioorg. Med. Chem., 16, 771782 (2008)

    • Search Google Scholar
    • Export Citation
  • [9]

    A.M. Innocente , G.N.S. Silva, L.N. Cruz, M.S. Moraes, M. Nakabashi, P. Sonnet, G. Gosmann, C.R.S. Garcia, and S.C.B. Gnoatto, Molecules, 17, 1200312014 (2012)

    • Search Google Scholar
    • Export Citation
  • [10]

    US Food and Drug Administration, Center for Drug Evaluation and Research, US Food and Drug Administration, Center for Drug Evaluation and Research, 2001. http://www.fda.gov/downloads/Drugs/Guidance/ComplianceRegulatoryInfor mation/Guidances/UCM070107.pdf

  • [11]

    L. Shargel , S. Wu-Pong, and A.B.C. Yu, Applied Biopharmaceutics and Pharmacokinetics, 5th edn., McGraw-Hill Book Co., New York, NY, 2005

    • Search Google Scholar
    • Export Citation
  • [12]

    M. Gibaldi and D. Perrier, Pharmacokinetics, 2nd edn., Marcel Dekker, New York, NY, 1982

  • [13]

    L. Zhang , M. Yu-Liang, L. Yang, and Z.J. Yuan-Gang, Chromatogr. B., 963, 6269 (2014)

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Senior editors

Editor(s)-in-Chief: Sajewicz, Mieczyslaw, University of Silesia, Katowice, Poland

Editors(s)

  • Danica Agbaba, University of Belgrade, Belgrade, Serbia (1953-2024)
  • Łukasz Komsta, Medical University of Lublin, Lublin, Poland
  • Ivana Stanimirova-Daszykowska, University of Silesia, Katowice, Poland
  • Monika Waksmundzka-Hajnos, Medical University of Lublin, Lublin, Poland

Editorial Board

  • Ravi Bhushan, The Indian Institute of Technology, Roorkee, India
  • Jacek Bojarski, Jagiellonian University, Kraków, Poland
  • Bezhan Chankvetadze, State University of Tbilisi, Tbilisi, Georgia
  • Michał Daszykowski, University of Silesia, Katowice, Poland
  • Tadeusz H. Dzido, Medical University of Lublin, Lublin, Poland
  • Attila Felinger, University of Pécs, Pécs, Hungary
  • Kazimierz Glowniak, Medical University of Lublin, Lublin, Poland
  • Bronisław Glód, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Anna Gumieniczek, Medical University of Lublin, Lublin, Poland
  • Urszula Hubicka, Jagiellonian University, Kraków, Poland
  • Krzysztof Kaczmarski, Rzeszow University of Technology, Rzeszów, Poland
  • Huba Kalász, Semmelweis University, Budapest, Hungary
  • Katarina Karljiković Rajić, University of Belgrade, Belgrade, Serbia
  • Imre Klebovich, Semmelweis University, Budapest, Hungary
  • Angelika Koch, Private Pharmacy, Hamburg, Germany
  • Piotr Kus, Univerity of Silesia, Katowice, Poland
  • Debby Mangelings, Free University of Brussels, Brussels, Belgium
  • Emil Mincsovics, Corvinus University of Budapest, Budapest, Hungary
  • Ágnes M. Móricz, Centre for Agricultural Research, Budapest, Hungary
  • Gertrud Morlock, Giessen University, Giessen, Germany
  • Anna Petruczynik, Medical University of Lublin, Lublin, Poland
  • Robert Skibiński, Medical University of Lublin, Lublin, Poland
  • Bernd Spangenberg, Offenburg University of Applied Sciences, Germany
  • Tomasz Tuzimski, Medical University of Lublin, Lublin, Poland
  • Adam Voelkel, Poznań University of Technology, Poznań, Poland
  • Beata Walczak, University of Silesia, Katowice, Poland
  • Wiesław Wasiak, Adam Mickiewicz University, Poznań, Poland
  • Igor G. Zenkevich, St. Petersburg State University, St. Petersburg, Russian Federation

 

SAJEWICZ, MIECZYSLAW
E-mail:mieczyslaw.sajewicz@us.edu.pl

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Journal Impact Factor 1.7
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Acta Chromatographica
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Acta Chromatographica
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
4
Founder Institute of Chemistry, University of Silesia
Founder's
Address
PL-40-007 Katowice, Poland, Bankowa 12
Publisher Akadémiai Kiadó
Publisher's
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H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2083-5736 (Online)

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