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  • 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • 2 Department of Pathology, School of Medicine, University of Zagreb, University Hospital Centre, Zagreb, Croatia
  • 3 Rudjer Boskovic Institute, NMR Center, Zagreb, Croatia
  • 4 Universitätsklinik für Unfallchirurgie, Medizinische Universität Graz, Graz, Austria
  • 5 Division of Molecular Medicine, Laboratory for Oxidative Stress, Rudjer Boskovic Institute, Bijenicka 54, Zagreb, Croatia
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Abstract

Tissue regeneration is a complex biological process of vital importance since it allows renewal of damaged cells and organs. The healing of long bones and large joints is often extended or incomplete primarily in elderly people or in polytraumatized patients. Various attempts are made to solve this severe medical and social problem by developing novel bioactive materials, among which bioactive glass is the most attractive because of its osteoconductive and osteostimulative properties. Lipid peroxidation is defined as an important parameter of systematic stress response in patients with traumatic brain injuries and bone fractures. The major bioactive marker and final product of lipid peroxidation, 4-hydroxynonenal (HNE), is a particularly interesting biomolecule because it regulates differentiation, proliferation and apoptosis of cells and might therefore play an important role in regulating the regeneration of damaged tissue such as bone. Therefore, in this study, we investigated the concept of using bioactive glass functionalized with HNE as an in vitro model of bone regeneration.

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