View More View Less
  • 1 Universitas Indonesia, Jakarta, Indonesia
  • | 2 Faculty of Veterinary, Institute Pertanian Bogor, Bogor, Indonesia
Open access

Objective

This study aims to evaluate the effect of soybean milk containing a combination of anti-Streptococcus mutans IgY and chitosan to the colonization of S. mutans in the saliva and to the IgY persistency in the saliva.

Materials and Methods

Experimental malnourished Sprague-Dawley rats were fed with soybean milk that is enriched with anti-S. mutans IgY and chitosan. After 15 days of feeding, we evaluated the S. mutans in dental biofilm, in addition to the persistency level of anti-S. mutans IgY.

Results

The rats that received soybean milk supplemented with anti-S. mutans IgY had the lowest number of S. mutans colonies (p < 0.05). Anti-S. mutans IgY was detected in saliva after 15 days of feeding.

Conclusions

Soybean milk supplemented with anti-S. mutans IgY and chitosan could significantly reduce S. mutans biofilm, and the supplemented anti-S. mutans IgY persisted in these rats’ saliva following the feeding period.

  • 1.

    Ward law TM: Low birthweight: country, regional and global estimates. UNICEF, 2004

  • 2.

    Rama krishnan U : Nutrition and low birth weight: from research to practice. Am J Clin Nutr 79, 1721 (2004)

  • 3.

    Drury TF , Winn DM, Snowden CB, Kingman A, Kleinman DV, Lewis B: An overview of the oral health component of the 1988–1991 National Health and Nutrition Examination Survey (NHANES III-Phase 1). J Dent Res 75, 620630 (1996)

    • Search Google Scholar
    • Export Citation
  • 4.

    Lee SM , Han HW, Yim SY: Beneficial effects of soy milk and fiber on high cholesterol diet-induced alteration of gut microbiota and inflammatory gene expression in rats. Food Funct 6, 492500 (2015)

    • Search Google Scholar
    • Export Citation
  • 5.

    Appukutty M , Ramasamy K, Rajan S, Vellasamy S, Ramasamy R, Radhakrishnan AK: Effect of orally administered soy milk fermented with Lactobacillus plantarum LAB12 and physical exercise on murine immune responses. Benef Microbes 6, 491496 (2015)

    • Search Google Scholar
    • Export Citation
  • 6.

    Font enla dPS , Prchal A, Fontenla M, Cena A, Gómez J, Pintos S, Peral MC: Recovery from experimental malnutrition with soymilk: immunological and genetic aspects. Nutr Hosp 22, 244251 (2006)

    • Search Google Scholar
    • Export Citation
  • 7.

    Indonesia DKR: Riset kesehatan dasar (RISKESDAS) Badan Penelitian dan Pengembangan Kesehatan, Jakarta, 2008

  • 8.

    Michalek SM , Gregory RL, Harmon CC, Katz J, Richardson GJ, Hilton T, Filler SJ, McGhee JR: Protection of gnotobiotic rats against dental caries by passive immunization with bovine milk antibodies to Streptococcus mutans. Infect Immun 55, 23412347 (1987)

    • Search Google Scholar
    • Export Citation
  • 9.

    Smith DJ , King WF, Godiska R: Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries. Infect Immun 69, 31353142 (2001)

    • Search Google Scholar
    • Export Citation
  • 10.

    Wei H , Loimaranta V, Tenovuo J, Rokka S, Syvaoja EL, Korhonen H, Joutsjoki V, Marnila P: Stability and activity of specific antibodies against Streptococcus mutans and Streptococcus sobrinus in bovine milk fermented with Lactobacillus rhamnosus strain GG or treated at ultra-high temperature. Oral Microbiol Immunol 17, 915 (2002)

    • Search Google Scholar
    • Export Citation
  • 11.

    Hoshino T , Kondo Y, Saito K, Terao Y, Okahashi N, Kawabata S, Fujiwara T: Novel epitopic region of glucosyltransferase B from Streptococcus mutans. Clin Vaccine Immunol 18, 15521561 (2011)

    • Search Google Scholar
    • Export Citation
  • 12.

    Chen L , Zhu J, Li Y, Lu J, Gao L, Xu H, Fan M, Yang X: Enhanced nasal mucosal delivery and immunogenicity of anti-caries DNA vaccine through incorporation of anionic liposomes in chitosan/ DNA complexes. PLoS One 8, e71953 (2013)

    • Search Google Scholar
    • Export Citation
  • 13.

    Yan H : Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine. Sci China Life Sci 56, 406413 (2013)

    • Search Google Scholar
    • Export Citation
  • 14.

    Kruger C , Hu Y, Pan Q, Marcotte H, Hultberg A, Delwar D, van Dalen PJ, Pouwels PH, Leer RJ, Kelly CG, van Dollenweerd C, Ma JK, Hammarstrom L: In situ delivery of passive immunity by lactobacilli producing single-chain antibodies. Nat Biotechnol 20, 702706 (2002)

    • Search Google Scholar
    • Export Citation
  • 15.

    Mitoma M , Oho T, Michibata N, Okano K, Nakano Y, Fukuyama M, Koga T: Passive immunization with bovine milk containing antibodies to a cell surface protein antigen-glucosyltransferase fusion protein protects rats against dental caries. Infect Immun 70, 27212724 (2002)

    • Search Google Scholar
    • Export Citation
  • 16.

    Fan M , Jiang Q, Bian Z: [The study on specific anti-Streptococcus mutans IgY against dental caries in rats]. Hua Xi Kou Qiang Yi Xue Za Zhi 21, 339341 (2003)

    • Search Google Scholar
    • Export Citation
  • 17.

    Robinette RA , Oli MW, McArthur WP, Brady LJ: A therapeutic anti-Streptococcus mutans monoclonal antibody used in human passive protection trials influences the adaptive immune response. Vaccine 29, 62926300 (2011)

    • Search Google Scholar
    • Export Citation
  • 18.

    Otake S , Nishihara Y, Makimura M, Hatta H, Kim M, Yamamoto T, Hirasawa M: Protection of rats against dental caries by passive immunization with hen-egg-yolk antibody (IgY). J Dent Res 70, 162166 (1991)

    • Search Google Scholar
    • Export Citation
  • 19.

    Hatta H , Tsuda K, Ozeki M, Kim M, Yamamoto T, Otake S, Hirasawa M, Katz J, Childers NK, Michalek SM: Passive immunization against dental plaque formation in humans: effect of a mouth rinse containing egg yolk antibodies (IgY) specific to Streptococcus mutans. Caries Res 31, 268274 (1997)

    • Search Google Scholar
    • Export Citation
  • 20.

    Carlander D , Kollberg H, Larsson A: Retention of specific yolk IgY in the human oral cavity. BioDrugs 16, 433437 (2002)

  • 21.

    Zhou Z , Zhou R, Tang Z: Effects of topical application of immunoglobulin yolk on mutans streptococci in dental plaque. Hua Xi Kou Qiang Yi Xue Za Zhi 21, 295297 (2003)

    • Search Google Scholar
    • Export Citation
  • 22.

    Chi ZB , Gao YX, Pan Y, Zhang B, Feng XP: The inhibitive effect of IgY toothpaste against oral Streptococcus mutans. Shanghai Kou Qiang Yi Xue 13, 256258 (2004)

    • Search Google Scholar
    • Export Citation
  • 23.

    Kruger C , Pearson SK, Kodama Y, Vacca Smith A, Bowen WH, Hammarstrom L: The effects of egg-derived antibodies to glucosyltransferases on dental caries in rats. Caries Res 38, 914 (2004)

    • Search Google Scholar
    • Export Citation
  • 24.

    Nguyen SV , Icatlo FC, Jr, Nakano T, Isogai E, Hirose K, Mizugai H, Kobayashi-Sakamoto M, Isogai H, Chiba I: Anti-cell-associated glucosyltransferase immunoglobulin Y suppression of salivary mutans streptococci in healthy young adults. J Am Dent Assoc 142, 943949 (2011)

    • Search Google Scholar
    • Export Citation
  • 25.

    Afdhal A , Bachtiar E, Meidyawati R: Reactivity of anti S. mutans IgY gel after 30 days. Conference paper, IADR General Session (2010)

  • 26.

    Shimizu M , Nagashima H, Sano K, Hashimoto K, Ozeki M, Tsuda K, Hatta H: Molecular stability of chicken and rabbit immunoglobulin G. Biosci Biotechnol Biochem 56, 270274 (1992)

    • Search Google Scholar
    • Export Citation
  • 27.

    Hayashi Y , Ohara N, Ganno T, Yamaguchi K, Ishizaki T, Nakamura T, Sato M: Chewing chitosan-containing gum effectively inhibits the growth of cariogenic bacteria. Arch Oral Biol 52, 290294 (2007)

    • Search Google Scholar
    • Export Citation
  • 28.

    Hsieh YL , Yao HT, Cheng RS, Chiang MT: Chitosan reduces plasma adipocytokines and lipid accumulation in liver and adipose tissues and ameliorates insulin resistance in diabetic rats. J Med Food 15, 453460 (2012)

    • Search Google Scholar
    • Export Citation
  • 29.

    Zhang HL , Zhong XB, Tao Y, Wu SH, Su ZQ: Effects of chitosan and water-soluble chitosan micro- and nanoparticles in obese rats fed a high-fat diet. Int J Nanomedicine 7, 40694076 (2012)

    • Search Google Scholar
    • Export Citation
  • 30.

    Zhang J , Zhang W, Mamadouba B, Xia W: A comparative study on hypolipidemic activities of high and low molecular weight chitosan in rats. Int J Biol Macromol 51, 504508 (2012)

    • Search Google Scholar
    • Export Citation
  • 31.

    Koga-Ito CY , Martins CA, Balducci I, Jorge AO: Correlation among mutans streptococci counts, dental caries, and IgA to Streptococcus mutans in saliva. Braz Oral Res 18, 350355 (2004)

    • Search Google Scholar
    • Export Citation
  • 32.

    Schenkels LC , Veerman EC, Nieuw Amerongen AV: Biochemical composition of human saliva in relation to other mucosal fluids. Crit Rev Oral Biol Med 6, 161175 (1995)

    • Search Google Scholar
    • Export Citation
  • 33.

    Chaudhuri B , Rojek J, Vickerman MM, Tanzer JM, Scannapieco FA: Interaction of salivary alpha-amylase and amylase-binding-protein A (AbpA) of Streptococcus gordonii with glucosyltransferase of S. gordonii and Streptococcus mutans. BMC Microbiol 7, 60 (2007)

    • Search Google Scholar
    • Export Citation
  • 34.

    Wei GX , Campagna AN, Bobek LA: Effect of MUC7 peptides on the growth of bacteria and on Streptococcus mutans biofilm. J Antimicrob Chemother 57, 11001109 (2006)

    • Search Google Scholar
    • Export Citation
  • 35.

    Wei GX , Campagna AN, Bobek LA: Factors affecting antimicrobial activity of MUC7 12-mer, a human salivary mucin-derived peptide. Ann Clin Microbiol Antimicrob 6, 14 (2007)

    • Search Google Scholar
    • Export Citation
  • 36.

    Sheikh C , Erickson P: Evaluation of plaque pH changes following oral rinse with eight infant formulas. Pediatric dentistry 18, 200204 (1996)

    • Search Google Scholar
    • Export Citation
  • 37.

    Akita EM , Nakai S: Isolation and purification of immunoglobulins from egg yolk. J Food Sci, 57, 629 (1992)

  • 38.

    Danchaivijitr A , Nakornchai S, Thaweeboon B, Leelataweewud P, Phonghanyudh A, Kiatprajak C, Surarit R: The effect of different milk formulas on dental plaque pH. Int J Paediatr Dent 16, 192198 (2006)

    • Search Google Scholar
    • Export Citation
  • 39.

    Tarsi R , Corbin B, Pruzzo C, Muzzarelli RA: Effect of low-molecular-weight chitosans on the adhesive properties of oral streptococci. Oral Microbiol Immunol 13, 217224 (1998)

    • Search Google Scholar
    • Export Citation
  • 40.

    Dashper SG , Saion BN, Stacey MA, Manton DJ, Cochrane NJ, Stanton DP, Yuan Y, Reynolds EC: Acidogenic potential of soy and bovine milk beverages. J Dent 40, 736741 (2012)

    • Search Google Scholar
    • Export Citation
  • 41.

    Shimizu M , Miwa Y, Hashimoto K, Goto A: Encapsulation of chicken egg yolk immunoglobulin G (IgY) by liposomes. Biosci Biotechnol Biochem 57, 14451449 (1993)

    • Search Google Scholar
    • Export Citation
  • 42.

    Shimizu M , Nakane Y: Encapsulation of biologically active proteins in a multiple emulsion. Biosci Biotechnol Biochem 59, 492496 (1995)

    • Search Google Scholar
    • Export Citation
  • 43.

    Chang HM , Ou-Yang RF, Chen YT, Chen CC: Productivity and some properties of immunoglobulin specific against Streptococcus mutans serotype c in chicken egg yolk (IgY). J Agric Food Chem 47, 6166 (1999)

    • Search Google Scholar
    • Export Citation
  • 44.

    Bizhanov G , Vyshniauskis G: A comparison of three methods for extracting IgY from the egg yolk of hens immunized with Sendai virus. Vet Res Commun 24, 103113 (2000)

    • Search Google Scholar
    • Export Citation

2019  
Scimago
H-index
11
Scimago
Journal Rank
0,220
Scimago
Quartile Score
Medicine (miscellaneous) Q3
Scopus
Cite Score
155/133=1,2
Scopus
Cite Score Rank
General Medicine 199/529 (Q2)
Scopus
SNIP
0,343
Scopus
Cites
206
Scopus
Documents
23

 

Interventional Medicine and Applied Science
Language English
Size  
Year of
Foundation
2009
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2061-1617 (Print)
ISSN 2061-5094 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2021 0 5 4
Jul 2021 0 9 10
Aug 2021 0 8 7
Sep 2021 0 4 3
Oct 2021 0 7 3
Nov 2021 0 14 9
Dec 2021 0 3 1