Authors:
A. Autengruber Helmholtz Centre for Infection Research Immune Regulation Group Braunschweig Germany

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M. Gereke

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G. Hansen Hannover Medical School Department of Pediatric Pneumology, Allergology and Neonatology Hannover Germany

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C. Hennig Hannover Medical School Department of Pediatric Pneumology, Allergology and Neonatology Hannover Germany

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Dunja Bruder

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Immunological characterization of immune cells that reside in specific anatomic compartments often requires their isolation from the respective tissue on the basis of enzymatic tissue disintegration. Applying enzymatic digestion of primary splenocytes, we evaluated the impact of collagenase and dispase, two enzymes that are commonly used for the liberation of immune cells from tissues, on the detectability of 48 immunologically relevant surface molecules that are frequently used for flow cytometric identification, isolation, and characterization of immune cell subsets. Whereas collagenase treatment had only minor effects on surface expression of most molecules tested, dispase treatment considerably affected antibody-mediated detectability of the majority of surface markers in subsequent FACS analyses. This effect was long lasting and, in case of high-dose dispase treatment, evident for the majority of surface molecules even after 24 h of in vitro culture. Of note, high-dose dispase treatment not only affected surface expression of certain molecules but also impaired antigen-specific proliferation of CD4+ and CD8+ T cells. Together, our data indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules with direct consequences for phenotypic analysis, FACS- and MACS-based target cell isolation, and immune cell function in cell culture experiments.

  • Kroemer G, Cuende E, Martinez C: Compartmentalization of the peripheral immune system. Adv Immunol 53, 157–216 (1993)

    Martinez C. , 'Compartmentalization of the peripheral immune system ' (1993 ) 53 Adv Immunol : 157 -216 .

    • Search Google Scholar
  • Arstila T et al.: Identical T cell clones are located within the mouse gut epithelium and lamina propia and circulate in the thoracic duct lymph. J Exp Med 191, 823–834 (2000)

    Arstila T. , 'Identical T cell clones are located within the mouse gut epithelium and lamina propia and circulate in the thoracic duct lymph ' (2000 ) 191 J Exp Med : 823 -834 .

    • Search Google Scholar
  • Bruder D et al.: CD4 T Lymphocyte-mediated lung disease: steady state between pathological and tolerogenic immune reactions. Am J Respir Crit Care Med 170, 1145–1152 (2004)

    Bruder D. , 'CD4 T Lymphocyte-mediated lung disease: steady state between pathological and tolerogenic immune reactions ' (2004 ) 170 Am J Respir Crit Care Med : 1145 -1152 .

    • Search Google Scholar
  • Derbinski J, Schulte A, Kyewski B, Klein, L: Promiscuous gene expression in medullary thymic epithelial cells mirrors the peripheral self. Nat Immunol 2, 1032–1039 (2001)

    Klein L. , 'Promiscuous gene expression in medullary thymic epithelial cells mirrors the peripheral self ' (2001 ) 2 Nat Immunol : 1032 -1039 .

    • Search Google Scholar
  • Sedgwick JD et al.: Isolation and direct characterization of resident microglial cells from the normal and inflamed central nervous system. Proc Natl Acad Sci U S A 88, 7438–7442 (1991)

    Sedgwick J.D. , 'Isolation and direct characterization of resident microglial cells from the normal and inflamed central nervous system ' (1991 ) 88 Proc Natl Acad Sci U S A : 7438 -7442 .

    • Search Google Scholar
  • Weigmann B et al.: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue. Nat Protoc 2, 2307–2311 (2007)

    Weigmann B. , 'Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue ' (2007 ) 2 Nat Protoc : 2307 -2311 .

    • Search Google Scholar
  • Westendorf AM et al.: CD4+ T cell mediated intestinal immunity: chronic inflammation versus immune regulation. Gut 54, 60–69 (2005)

    Westendorf A.M. , 'CD4+ T cell mediated intestinal immunity: chronic inflammation versus immune regulation ' (2005 ) 54 Gut : 60 -69 .

    • Search Google Scholar
  • Corti M, Brody AR, Harrison JH: Isolation and primary culture of murine alveolar type II cells. Am J Respir Cell Mol Biol 14, 309–315 (1996)

    Harrison J.H. , 'Isolation and primary culture of murine alveolar type II cells ' (1996 ) 14 Am J Respir Cell Mol Biol : 309 -315 .

    • Search Google Scholar
  • Gereke M, Jung S, Buer J, Bruder D: Alveolar type II epithelial cells present antigen to CD4(+) T cells and induce Foxp3(+) regulatory T cells. Am J Respir Crit Care Med 179, 344–355 (2009)

    Bruder D. , 'Alveolar type II epithelial cells present antigen to CD4(+) T cells and induce Foxp3(+) regulatory T cells ' (2009 ) 179 Am J Respir Crit Care Med : 344 -355 .

    • Search Google Scholar
  • Lienenklaus S et al.: Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo. J Immunol 183, 3229–3236 (2009)

    Lienenklaus S. , 'Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo ' (2009 ) 183 J Immunol : 3229 -3236 .

    • Search Google Scholar
  • Ford AL, Foulcher E, Goodsall AL, Sedgwick JD: Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression. J Immunol Methods 194, 71–75 (1996)

    Sedgwick J.D. , 'Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression ' (1996 ) 194 J Immunol Methods : 71 -75 .

    • Search Google Scholar
  • White HD et al.: A method for the dispersal and characterization of leukocytes from the human female reproductive tract. Am J Reprod Immunol 44, 96–103 (2000)

    White H.D. , 'A method for the dispersal and characterization of leukocytes from the human female reproductive tract ' (2000 ) 44 Am J Reprod Immunol : 96 -103 .

    • Search Google Scholar
  • Gereke M et al.: Phenotypic alterations in type II alveolar epithelial cells in CD4+ T cell mediated lung inflammation. Respir Res 8, 47 (2007)

    Gereke M. , 'Phenotypic alterations in type II alveolar epithelial cells in CD4+ T cell mediated lung inflammation ' (2007 ) 8 Respir Res : 47 -.

    • Search Google Scholar
  • Kirberg J et al.: Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor. J Exp Med 180, 25–34 (1994)

    Kirberg J. , 'Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor ' (1994 ) 180 J Exp Med : 25 -34 .

    • Search Google Scholar
  • Morgan DJ et al.: CD8(+) T cell-mediated spontaneous diabetes in neonatal mice. J Immunol 157, 978–983 (1996)

    Morgan D.J. , 'CD8(+) T cell-mediated spontaneous diabetes in neonatal mice ' (1996 ) 157 J Immunol : 978 -983 .

    • Search Google Scholar
  • Holt PG et al.: Extraction of immune and inflammatory cells from human lung parenchyma: evaluation of an enzymatic digestion procedure. Clin Exp Immunol 66, 188–200 (1986)

    Holt P.G. , 'Extraction of immune and inflammatory cells from human lung parenchyma: evaluation of an enzymatic digestion procedure ' (1986 ) 66 Clin Exp Immunol : 188 -200 .

    • Search Google Scholar
  • Hennig C, Adams N, Hansen G: A versatile platform for comprehensive chip-based explorative cytometry. Cytometry A 75, 362–370 (2009)

    Hansen G. , 'A versatile platform for comprehensive chip-based explorative cytometry ' (2009 ) 75 Cytometry A : 362 -370 .

    • Search Google Scholar
  • Schubert W et al.: Analyzing proteome topology and function by automated multidimensional fluorescence microscopy. Nat Biotechnol 24, 1270–1278 (2006)

    Schubert W. , 'Analyzing proteome topology and function by automated multidimensional fluorescence microscopy ' (2006 ) 24 Nat Biotechnol : 1270 -1278 .

    • Search Google Scholar
  • Bland PW, Richens ER, Britton DC, Lloyd JV: Isolation and purification of human large bowel mucosal lymphoid cells: effect of separation technique on functional characteristics. Gut 20, 1037–1046 (1979)

    Lloyd J.V. , 'Isolation and purification of human large bowel mucosal lymphoid cells: effect of separation technique on functional characteristics ' (1979 ) 20 Gut : 1037 -1046 .

    • Search Google Scholar
  • Gascoigne NR, Zal T, Yachi PP, Hoerter JA: Co-receptors and recognition of self at the immunological synapse. Curr Top Microbiol Immunol 340, 171–189 (2010)

    Hoerter J.A. , 'Co-receptors and recognition of self at the immunological synapse ' (2010 ) 340 Curr Top Microbiol Immunol : 171 -189 .

    • Search Google Scholar
  • Hendriks J, Xiao Y, Borst J: CD27 promotes survival of activated T cells and complements CD28 in generation and establishment of the effector T cell pool. J Exp Med 198, 1369–1380 (2003)

    Borst J. , 'CD27 promotes survival of activated T cells and complements CD28 in generation and establishment of the effector T cell pool ' (2003 ) 198 J Exp Med : 1369 -1380 .

    • Search Google Scholar
  • Van PL et al.: Functional responses and apoptosis of CD25 (IL-2R alpha)-deficient T cells expressing a transgenic antigen receptor. J Immunol 158, 3738–3745 (1997)

    Van P.L. , 'Functional responses and apoptosis of CD25 (IL-2R alpha)-deficient T cells expressing a transgenic antigen receptor ' (1997 ) 158 J Immunol : 3738 -3745 .

    • Search Google Scholar
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Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita, Prof. Dr. Pharm, Dr. rer. nat., University of Magdeburg, Germany

Editor(s)-in-Chief: Heimesaat, Markus M., Prof. Dr. med., Charité - University Medicine Berlin, Germany

Editorial Board

  • Berit Bangoura, Dr. DVM. PhD,  University of Wyoming, USA
  • Stefan Bereswill, Prof. Dr. rer. nat., Charité - University Medicine Berlin, Germany
  • Dunja Bruder, Prof. Dr. rer. nat., University of Magdeburg, Germany
  • Jan Buer, Prof. Dr. med., University of Duisburg, Germany
  • Edit Buzas, Prof. Dr. med., Semmelweis University, Hungary
  • Renato Damatta, Prof. PhD, UENF, Brazil
  • Maria Deli, MD, PhD, DSc, Biological Research Center, HAS, Hungary
  • Olgica Djurković-Djaković, Prof. Phd, University of Belgrade, Serbia
  • Jean-Dennis Docquier, Prof. Dr. med., University of Siena, Italy
  • Zsuzsanna Fabry, Prof. Phd, University of Washington, USA
  • Ralf Ignatius, Prof. Dr. med., Charité - University Medicine Berlin, Germany
  • Achim Kaasch, Prof. Dr. med., Otto von Guericke University Magdeburg, Germany
  • Oliver Liesenfeld, Prof. Dr. med., Inflammatix, USA
  • Matyas Sandor, Prof. PhD, University of Wisconsin, USA
  • Ulrich Steinhoff, Prof. PhD, University of Marburg, Germany
  • Michal Toborek, Prof. PhD, University of Miami, USA
  • Susanne A. Wolf, PhD, MDC-Berlin, Germany

 

Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildiko.dunay@med.ovgu.de

Indexing and Abstracting Services:

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2024  
Scopus  
CiteScore  
CiteScore rank  
SNIP  
Scimago  
SJR index 0.569
SJR Q rank Q3

2023  
Web of Science  
Total Cites
WoS
674
Journal Impact Factor 3.3
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Q2

Impact Factor
without
Journal Self Cites
3.1
5 Year
Impact Factor
3.2
Scimago  
Scimago
H-index
15
Scimago
Journal Rank
0.601
Scimago Quartile Score Microbiology (medical) (Q2)
Microbiology (Q3)
Immunology and Allergy (Q3)
Immunology (Q3)
Scopus  
Scopus
Cite Score
5.0
Scopus
CIte Score Rank
Microbiology (medical) Q2
Scopus
SNIP
0.832

 

European Journal of Microbiology and Immunology
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European Journal of Microbiology and Immunology
Language English
Size A4
Year of
Foundation
2011
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2062-509X (Print)
ISSN 2062-8633 (Online)

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