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  • 1 Sapporo Medical University Hokkaido Japan
  • | 2 University of Occupational and Environmental Health Fukuoka Japan
  • | 3 Kobe University Hospital Hyogo Japan
  • | 4 2-6-5 Fushimi Tyuo-ku Sapporo City Hokkaido 064-0942 Japan
Open access

This prospective, multicenter clinical trial was conducted to compare the performance of the cobas® 4800 CT/NG, APTIMA Combo 2®, and ProbeTec™ ET CT/GC assays for the detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in Japan. From 1274 male and female patients, more than 1900 urine, endocervical and throat specimens were collected. Positive and negative concordance rates for CT and NG results obtained for urine and endocervical samples collected from the same patient were high in all three assays (range 96.0–99.6%). The accuracy of the cobas® 4800 CT/NG test did not differ significantly from that of the APTIMA Combo 2® and ProbeTec™ ET CT/GC assays. The accuracy of the assays did not change depending on the order of collection of endocervical specimens. Concordance rates for results obtained for throat swabs and mouthwashes in the ProbeTec™ ET CT/GC and cobas® 4800 CT/NG assays, respectively, were 98.8% for CT and 95.1% for NG. These data suggest that the cobas®4800 CT/NG test is a reliable and highly accurate diagnostic tool for the detection of CT and NG in urine, genital, and oral specimens. Owing to the high correlation of urine and endocervical swab results and the ease of acquisition, urine samples are suggested as the specimen of choice for screening of CT and NG.

  • Tanaka M: Recent trends of sexually transmitted diseases in Fukuoka city. Jpn Arch STD 4, 39–46 (1993)

    Tanaka M. , 'Recent trends of sexually transmitted diseases in Fukuoka city ' (1993 ) 4 Jpn Arch STD : 39 -46.

    • Search Google Scholar
  • Japan SGfSsi: STD surveillance 2001 in Japan. Jpn J Sex Transm Dis 13, 147–167 (2002)

    'STD surveillance 2001 in Japan ' (2002 ) 13 Jpn J Sex Transm Dis : 147 -167.

  • Imai H, Nakao H, Shinohara H, Fujii Y, Tsukino H et al.: Population-based study of asymptomatic infection with Chlamydia trachomatis among female and male students. Int J STD AIDS 21, 362–366 (2010)

    Tsukino H. , 'Population-based study of asymptomatic infection with Chlamydia trachomatis among female and male students ' (2010 ) 21 Int J STD AIDS : 362 -366.

    • Search Google Scholar
  • Matsumoto T: Trends of sexually transmitted diseases and antimicrobial resistance in Neisseria gonorrhoeae. Int J Antimicrob Agents 31(Suppl 1), S35–S39 (2008)

    Matsumoto T. , 'Trends of sexually transmitted diseases and antimicrobial resistance in Neisseria gonorrhoeae ' (2008 ) 31 Int J Antimicrob Agents : S35 -S39.

    • Search Google Scholar
  • Tsunoe H, Tanaka M, Nakayama H, Sano M, Nakamura G et al.: High prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in female commercial sex workers in Japan. Int J STD AIDS 11, 790–794 (2000)

    Nakamura G. , 'High prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in female commercial sex workers in Japan ' (2000 ) 11 Int J STD AIDS : 790 -794.

    • Search Google Scholar
  • Imai H, Shinohara H, Nakao H, Tsukino H, Hamasuna R et al.: Prevalence and risk factors of asymptomatic chlamydial infection among students in Japan. Int J STD AIDS 15, 408–414 (2004)

    Hamasuna R. , 'Prevalence and risk factors of asymptomatic chlamydial infection among students in Japan ' (2004 ) 15 Int J STD AIDS : 408 -414.

    • Search Google Scholar
  • Tabrizi SN, Unemo M, Limnios AE, Hogan TR, Hjelmevoll SO et al.: Evaluation of six commercial nucleic acid amplification tests for the detection of Neisseria gonorrhoeae and other Neisseria species. J Clin Microbiol 49, 3610–3615 (2011)

    Hjelmevoll S.O. , 'Evaluation of six commercial nucleic acid amplification tests for the detection of Neisseria gonorrhoeae and other Neisseria species ' (2011 ) 49 J Clin Microbiol : 3610 -3615.

    • Search Google Scholar
  • Rockett R, Goire N, Limnios A, Turra M, Higgens G et al.: Evaluation of the cobas 4800 CT/NG test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae. Sex Transm Infect 86, 470–473 (2010)

    Higgens G. , 'Evaluation of the cobas 4800 CT/NG test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae ' (2010 ) 86 Sex Transm Infect : 470 -473.

    • Search Google Scholar
  • Gaydos CA, Cartwright CP, Colaninno P, Welsch J, Holden J et al.: Performance of the Abbott RealTime CT/NG for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol 48, 3236–3243 (2010)

    Holden J. , 'Performance of the Abbott RealTime CT/NG for detection of Chlamydia trachomatis and Neisseria gonorrhoeae ' (2010 ) 48 J Clin Microbiol : 3236 -3243.

    • Search Google Scholar
  • Taylor SN, Van Der Pol B, Lillis R, Hook EW, 3rd, Lebar W et al.: Clinical evaluation of the BD ProbeTec Chlamydia trachomatis Qx amplified DNA assay on the BD Viper system with XTR technology. Sex Transm Dis 38, 603–609 (2011)

    Lebar W. , 'Clinical evaluation of the BD ProbeTec Chlamydia trachomatis Qx amplified DNA assay on the BD Viper system with XTR technology ' (2011 ) 38 Sex Transm Dis : 603 -609.

    • Search Google Scholar
  • Gaydos CA, Quinn TC, Willis D, Weissfeld A, Hook EW et al.: Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J Clin Microbiol 41, 304–309 (2003)

    Hook E.W. , 'Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens ' (2003 ) 41 J Clin Microbiol : 304 -309.

    • Search Google Scholar
  • Schachter J, Chernesky MA, Willis DE, Fine PM, Martin DH et al.: Vaginal swabs are the specimens of choice when screening for Chlamydia trachomatis and Neisseria gonorrhoeae: results from a multicenter evaluation of the APTIMA assays for both infections. Sex Transm Dis 32, 725–728 (2005)

    Martin D.H. , 'Vaginal swabs are the specimens of choice when screening for Chlamydia trachomatis and Neisseria gonorrhoeae: results from a multicenter evaluation of the APTIMA assays for both infections ' (2005 ) 32 Sex Transm Dis : 725 -728.

    • Search Google Scholar
  • Schachter J, Moncada J, Liska S, Shayevich C, Klausner J: Nucleic acid amplification tests in the diagnosis of chlamydial and gonococcal infections of the oropharynx and rectum in men who have sex with men. Sex Transm Dis 35, 637–642 (2008)

    Klausner J. , 'Nucleic acid amplification tests in the diagnosis of chlamydial and gonococcal infections of the oropharynx and rectum in men who have sex with men ' (2008 ) 35 Sex Transm Dis : 637 -642.

    • Search Google Scholar
  • Walker CK, Sweet RL: Gonorrhea infection in women: prevalence, effects, screening, and management. Int J Womens Health 3, 197–206 (2011)

    Sweet R.L. , 'Gonorrhea infection in women: prevalence, effects, screening, and management ' (2011 ) 3 Int J Womens Health : 197 -206.

    • Search Google Scholar
  • Papp JR, Ahrens K, Phillips C, Kent CK, Philip S et al.: The use and performance of oral-throat rinses to detect pharyngeal Neisseria gonorrhoeae and Chlamydia trachomatis infections. Diagn Microbiol Infect Dis 59, 259–264 (2007)

    Philip S. , 'The use and performance of oral-throat rinses to detect pharyngeal Neisseria gonorrhoeae and Chlamydia trachomatis infections ' (2007 ) 59 Diagn Microbiol Infect Dis : 259 -264.

    • Search Google Scholar
  • Fairley CK, Chen MY, Bradshaw CS, Tabrizi SN: Is it time to move to nucleic acid amplification tests screening for pharyngeal and rectal gonorrhoea in men who have sex with men to improve gonorrhoea control? Sex Health 8, 9–11 (2011)

    Tabrizi S.N. , 'Is it time to move to nucleic acid amplification tests screening for pharyngeal and rectal gonorrhoea in men who have sex with men to improve gonorrhoea control? ' (2011 ) 8 Sex Health : 9 -11.

    • Search Google Scholar
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Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Vice Editor(s)-in-Chief: Fuchs, Anja

Editorial Board

Chair of the Editorial Board:
Jeffrey S. Buguliskis (Thomas Jefferson University, USA)

  • Jörn Albring (University of Münster, Germany)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Megdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Jeff Buguliskis (Thomas Jefferson University, USA)
  • Edit Buzas (Semmelweis University, Hungary)
  • Charles Collyer (University of Sydney, Australia)
  • Renato Damatta (UENF, Brazil)
  • Ivelina Damjanova (Semmelweis University, Hungary)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Anna Erdei (Eötvös Loránd University, Hungary)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Beniam Ghebremedhin (Witten/Herdecke University, Germany)
  • Nancy Guillen (Institute Pasteur, France)
  • Georgina L. Hold (University of Aberdeen, United Kingdom)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Zsuzsanna Izsvak (MDC-Berlin, Germany)
  • Achim Kaasch (University of Cologne, Germany)
  • Tamás Laskay (University of Lübeck, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Shreemanta Parida (Vaccine Grand Challenge Program, India)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Mary Jo Wick (University of Gothenburg, Sweden)
  • Susanne A. Wolf (MDC-Berlin, Germany)

 

Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildikodunay@gmail.com

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2020  
CrossRef Documents 23
WoS Cites 708
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Days from submission to acceptance 219
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Acceptance Rate 70%

2019  
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CrossRef
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Acceptance
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European Journal of Microbiology and Immunology
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European Journal of Microbiology and Immunology
Language English
Size A4
Year of
Foundation
2011
Publication
Programme
2021 Volume 11
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
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H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Publisher's
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H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Chief Executive Officer, Akadémiai Kiadó
ISSN 2062-509X (Print)
ISSN 2062-8633 (Online)

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