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Miles Beaman

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Mahdad Karimi Western Diagnostic Pathology Department of Microbiology 74 McCoy St. Myaree Western Australia 6154 Australia

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Meredith Hodge Western Diagnostic Pathology Department of Microbiology 74 McCoy St. Myaree Western Australia 6154 Australia

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Anthony Keil Princess Margaret Hospital for Children Department of Microbiology, PathWest Laboratory Medicine WA Thomas St. Subiaco Western Australia 6008 Australia

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Peter Campbell Princess Margaret Hospital for Children Department of Microbiology, PathWest Laboratory Medicine WA Thomas St. Subiaco Western Australia 6008 Australia

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We assessed IgA antibodies and polymerase chain reaction (PCR) for diagnosis of pertussis in nasopharyngeal aspiration (NPA) samples from outpatients in Australia.A total of 1700 patients (849 adults, 851 children) from Western Australia and the Northern Territory fulfilled the laboratory case definition for pertussis between 2004 and 2013: 732 specimens were positive by NPA IgA alone, 559 by PCR alone, and 409 by both tests. Overall, 968 cases (56.8%) were positive by PCR and 1141 cases (67.2%) by IgA [p < 0.00025]. Among pediatric patients, PCR was positive in 524 (61.3%) and IgA in 569 (67%). In 849 adult cases, the respective proportions were 52.3% and 67.4% [p < 0.00025].The duration of cough in 507 patients was shorter in 262 pediatric cases (mean, 2.51 weeks; standard deviation [SD], 2.25) than 245 adult patients (3.27 weeks; SD, 2.79) [p = 0.0009]. PCR positivity showed a season-dependent variance (range, 5.6 to 85.9%) and peaked in the second week (71.7%) of illness. IgA antibodies peaked in the fifth week (89.5%) postinfection, and the positivity rate for NPA IgA was less variable (range, 38.3–97.2%).Nasopharyngeal Bordetella pertussis-specific IgA antibodies are valuable in diagnosis of pertussis in Australia. Reliance on PCR alone misses a significant proportion of pertussis cases, especially those with a delayed presentation.

  • De Serres G, Shadmani R, Duval B, Boulianne N, Dery P, Fradet MD, Rochette L, Halperin SA: Morbidity of pertussis in adolescents and adults. J Infect Dis 182, 174–179 (2000)

    Halperin SA , 'Morbidity of pertussis in adolescents and adults ' (2000 ) 182 J Infect Dis : 174 -179 .

    • Search Google Scholar
  • Dworkin M: Adults are whooping, but are internists listening? Ann Intern Med 142, 832–835 (2005)

    Dworkin M , 'Adults are whooping, but are internists listening ' (2005 ) 142 Ann Intern Med : 832 -835 .

    • Search Google Scholar
  • Forsyth K: Pertussis, still a formidable foe. Clin Infect Dis 45, 1487–1491 (2007)

    Forsyth K , 'Pertussis, still a formidable foe ' (2007 ) 45 Clin Infect Dis : 1487 -1491 .

    • Search Google Scholar
  • Cherry J: The present and future control of pertussis. Clin Infect Dis 51, 663–667 (2010)

    Cherry J , 'The present and future control of pertussis ' (2010 ) 51 Clin Infect Dis : 663 -667 .

    • Search Google Scholar
  • Halperin SA, Bortolus R, Wort AJ: Evaluation of culture, immunofluorescence and serology for diagnosis of pertussis. J Clin Microbiol 27, 752–757 (1989)

    Wort AJ , 'Evaluation of culture, immunofluorescence and serology for diagnosis of pertussis ' (1989 ) 27 J Clin Microbiol : 752 -757 .

    • Search Google Scholar
  • Goodman YE, Wort AJ, Jackson FL: Enzyme-linked immunosorbant assay for detection of Pertussis immunoglobulin A in nasopharyngeal secretions as an indicator of recent infection. J Clin Microbiol 13, 286–292 (1981)

    Jackson FL , 'Enzyme-linked immunosorbant assay for detection of Pertussis immunoglobulin A in nasopharyngeal secretions as an indicator of recent infection ' (1981 ) 13 J Clin Microbiol : 286 -292 .

    • Search Google Scholar
  • Granstrom G, Askelof P, Granstrom M: Specific immunoglobulin A to Bordetella pertussis antigens in mucosal secretion for rapid diagnosis of whooping cough. J Clin Microbiol 26, 869–874 (1988)

    Granstrom M , 'Specific immunoglobulin A to Bordetella pertussis antigens in mucosal secretion for rapid diagnosis of whooping cough ' (1988 ) 26 J Clin Microbiol : 869 -874 .

    • Search Google Scholar
  • Campbell PB, Masters PL, Rohwedder E: Whooping cough diagnosis: a clinical evaluation of complementing culture and immunofluorescence with enzyme-linked immunosorbent assay of pertussis immunoglobulin A in nasopharyngeal secretions. J Med Microbiol 27, 247–254 (1988)

    Rohwedder E , 'Whooping cough diagnosis: a clinical evaluation of complementing culture and immunofluorescence with enzyme-linked immunosorbent assay of pertussis immunoglobulin A in nasopharyngeal secretions ' (1988 ) 27 J Med Microbiol : 247 -254 .

    • Search Google Scholar
  • Glare EM, Patton JC, Premier RR, Lawrence AJ, Nisbett IT: Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction. J Clin Microbiol 28, 1982–1987 (1990)

    Nisbett IT , 'Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction ' (1990 ) 28 J Clin Microbiol : 1982 -1987 .

    • Search Google Scholar
  • Loeffelholz MJ, Thompson C, Long KS, Gilchrist MJR: Comparison of PCR, culture and direct fluorescent-anti-body for detection of Bordetella pertussis. J Clin Microbiol 37, 2872–2876 (1999)

    Gilchrist MJR , 'Comparison of PCR, culture and direct fluorescent-anti-body for detection of Bordetella pertussis ' (1999 ) 37 J Clin Microbiol : 2872 -2876 .

    • Search Google Scholar
  • Palmer CM, McCall B, Jarvinen K, Nissen MD: Bordetella pertussis PCR positivity, following onset of illness in children under 5 years of age. Commun Dis Intell Q Rep 31, 202–205 (2007)

    Nissen MD , 'Bordetella pertussis PCR positivity, following onset of illness in children under 5 years of age ' (2007 ) 31 Commun Dis Intell Q Rep : 202 -205 .

    • Search Google Scholar
  • Lievano FA, Reynolds MA, Waring AL, Ackelsberg J, Bisgard KM, Sanden GN, Guris D, Golaz A, Bopp DJ, Limberger RJ, Smith PF: Issues associated with and recommendations for using PCR to detect outbreaks of pertussis. J Clin Microbiol 40, 2801–2805 (2002)

    Smith PF , 'Issues associated with and recommendations for using PCR to detect outbreaks of pertussis ' (2002 ) 40 J Clin Microbiol : 2801 -2805 .

    • Search Google Scholar
  • Kirkland K, Talbot EA, Lasky RA, McLellan RK, Montero JT, Barry MA, McCarthy TA, Gunn JE, Pendarvis JL, Han LL, Devasia RA, Edwards KM, Jones TF, Kretsinger K, Tondella ML, Tatti KM, Brown KH, Slade BA, Wu K-H, Lu X, Patel M: Outbreaks of respiratory illness mistakenly attributed to Pertussis: New Hampshire, Massachusetts and Tennessee 2004–6. MMWR 56, 837–842 (2007)

    Lu X P M , 'Outbreaks of respiratory illness mistakenly attributed to Pertussis: New Hampshire, Massachusetts and Tennessee 2004–6 ' (2007 ) 56 MMWR : 837 -842 .

    • Search Google Scholar
  • Australian Government, Department of Health and Ageing: National Notifiable Diseases Surveillance System. Pertussis 2010, http://www9.health.gov.au/cda/Source/Rpt_4_sel.cfm (accessed 25.9.14)

  • Webby R: Pertussis program for new parents. North Territ Commun Dis Bull 15, 20–21 (2008)

    Webby R , 'Pertussis program for new parents ' (2008 ) 15 North Territ Commun Dis Bull : 20 -21 .

    • Search Google Scholar
  • Australian Government, Department of Health and Ageing. PERTUSSIS: national guidelines for public health units. http://www.health.gov.au/internet/main/publishing.nsf/content/056DAAB5640EFD2FCA25755E007629CC/$File/pertussis-song.pdf, 2010 (accessed 25.9.14)

  • Jasinska EM, Campbell BP, Blake BP: Evaluation of a commercial Bordetella pertussis serum immunoglobulin A (IgA) enzyme-linked immunosorbent assay (ELISA) for detection of pertussis mucosal IgA in nasopharyngeal secretions. Abstract no. P09.11 in Annual Scientific Meeting, Australian Society for Microbiology. Melbourne (2002)

    Blake BP , '', in Melbourne , (2002 ) -.

  • Kosters K, Reischl U, Schmetz J, Riffelmann M, Wirsig von Konig CH: Real-time LightCycler PCR for detection and discrimination of Bordetella pertussis and Bordetella parapertussis. J Clin Microbiol 40, 1719–1722 (2002)

    Wirsig von Konig CH , 'Real-time LightCycler PCR for detection and discrimination of Bordetella pertussis and Bordetella parapertussis ' (2002 ) 40 J Clin Microbiol : 1719 -1722 .

    • Search Google Scholar
  • Dawson-Saunders B, Trapp RG (1990): Basic and clinical statistics. Appleton and Lange, San Mateo, CA

    Trapp RG , '', in Basic and clinical statistics , (1990 ) -.

  • Australian Government, Department of Health and Ageing: Surveillance Case Definitions for the Australian National Notifiable Diseases Surveillance System. http://www.health.gov.au/internet/main/publishing.nsf/Content/cdna-casedefinitions.htm/$File/casedef-V4.pdf, 2011 (accessed 25.9.14)

  • Riffelmann M, Wirsing von Konig CH, Caro V, Guiso N, the Pertussis PCR Consensus Group: Nucleic acid amplification tests for diagnosis of Bordetella infections. J Clin Microbiol 43, 4925–4929 (2005)

    Guiso N , 'Nucleic acid amplification tests for diagnosis of Bordetella infections ' (2005 ) 43 J Clin Microbiol : 4925 -4929 .

    • Search Google Scholar
  • Cox HC, Jacob K, Whiley DM, Bletchly C, Nimmo GR, Nissen MD, Sloots TP: Further evidence that the IS481 target is suitable for real-time PCR detection of Bordetella pertussis. Pathology 45, 202–203 (2013)

    Sloots TP , 'Further evidence that the IS481 target is suitable for real-time PCR detection of Bordetella pertussis ' (2013 ) 45 Pathology : 202 -203 .

    • Search Google Scholar
  • Cox HC, Jacob K, Whiley DM, Bletchly C, Nimmo GR, Nissen MD, Sloots TP: Bordetella holmesii and pertussis diagnosis: authors’ reply. Pathology 45, 532 (2013)

    Sloots TP , 'Bordetella holmesii and pertussis diagnosis: authors’ reply ' (2013 ) 45 Pathology : 532 -.

    • Search Google Scholar
  • Abu-Diab A, Azzeh M, Ghneim R, Ghneim R, Zoughbi M, Turkuman S, Rishmawi N, Issa A, Siriani I, Dauodi R, Kattan R, Hindiyeh M: Comparison between pernasal flocked swabs and nasopharyngeal aspirates for detection of common respiratory viruses in samples from children. J Clin Microbiol 46, 2414–2417 (2008)

    Hindiyeh M , 'Comparison between pernasal flocked swabs and nasopharyngeal aspirates for detection of common respiratory viruses in samples from children ' (2008 ) 46 J Clin Microbiol : 2414 -2417 .

    • Search Google Scholar
  • Guiso N, Berbers G, Fry NK, He Q, Riffelmann M, Wirsing von Konig CH, EU Pertstrain Group: What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories. Eur J Clin Microbiol Infect Dis 30, 307–312 (2011)

    Wirsing von Konig CH , 'What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories ' (2011 ) 30 Eur J Clin Microbiol Infect Dis : 307 -312 .

    • Search Google Scholar
  • Poynten IM, Hanlon M, Irwig L, Gilbert GL: Serological diagnosis of pertussis: evaluation of IgA against whole cell and specific Bordetella pertussis antigens as markers of recent infection. Epidemiol Infect 128, 161–167 (2002)

    Gilbert GL , 'Serological diagnosis of pertussis: evaluation of IgA against whole cell and specific Bordetella pertussis antigens as markers of recent infection ' (2002 ) 128 Epidemiol Infect : 161 -167 .

    • Search Google Scholar
  • May ML, Doi S, King D, Evans J, Robson JM: Prospective evaluation of an Australian pertussis toxin IgG and IgA enzyme immunoassay. Clin Vaccine Immunol 19, 190–197 (2012)

    Robson JM , 'Prospective evaluation of an Australian pertussis toxin IgG and IgA enzyme immunoassay ' (2012 ) 19 Clin Vaccine Immunol : 190 -197 .

    • Search Google Scholar
  • Litt DJ, Samuel D, Duncan J, Harnden A, George RC, Harrison TG: Detection of anti-pertussis toxin IgG in oral fluids for use in diagnosis and surveillance of Bordetella pertussis infection in children and young adults. J Med Microbiol 55, 1223–1228 (2006)

    Harrison TG , 'Detection of anti-pertussis toxin IgG in oral fluids for use in diagnosis and surveillance of Bordetella pertussis infection in children and young adults ' (2006 ) 55 J Med Microbiol : 1223 -1228 .

    • Search Google Scholar
  • Australian Government, Department of Health and Ageing. http://www9.health.gov.au/cda/Source/Rpt_4_sel.cfm, in National Notifiable Diseases Surveillance System. 2010 (accessed 25.9.14)

  • Hallander HO, Reizenstein E, Renemar B, Rasmuson G, Mardin L, Olin P: Comparison of nasopharyngeal aspirates with swabs for culture of Bordetella pertussis. J Clin Microbiol 31, 50–52 (1993)

    Olin P , 'Comparison of nasopharyngeal aspirates with swabs for culture of Bordetella pertussis ' (1993 ) 31 J Clin Microbiol : 50 -52 .

    • Search Google Scholar
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Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita, Prof. Dr. Pharm, Dr. rer. nat., University of Magdeburg, Germany

Editor(s)-in-Chief: Heimesaat, Markus M., Prof. Dr. med., Charité - University Medicine Berlin, Germany

Editorial Board

  • Berit Bangoura, Dr. DVM. PhD,  University of Wyoming, USA
  • Stefan Bereswill, Prof. Dr. rer. nat., Charité - University Medicine Berlin, Germany
  • Dunja Bruder, Prof. Dr. rer. nat., University of Magdeburg, Germany
  • Jan Buer, Prof. Dr. med., University of Duisburg, Germany
  • Edit Buzas, Prof. Dr. med., Semmelweis University, Hungary
  • Renato Damatta, Prof. PhD, UENF, Brazil
  • Maria Deli, MD, PhD, DSc, Biological Research Center, HAS, Hungary
  • Olgica Djurković-Djaković, Prof. Phd, University of Belgrade, Serbia
  • Jean-Dennis Docquier, Prof. Dr. med., University of Siena, Italy
  • Zsuzsanna Fabry, Prof. Phd, University of Washington, USA
  • Ralf Ignatius, Prof. Dr. med., Charité - University Medicine Berlin, Germany
  • Achim Kaasch, Prof. Dr. med., Otto von Guericke University Magdeburg, Germany
  • Oliver Liesenfeld, Prof. Dr. med., Inflammatix, USA
  • Matyas Sandor, Prof. PhD, University of Wisconsin, USA
  • Ulrich Steinhoff, Prof. PhD, University of Marburg, Germany
  • Michal Toborek, Prof. PhD, University of Miami, USA
  • Susanne A. Wolf, PhD, MDC-Berlin, Germany

 

Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildiko.dunay@med.ovgu.de

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European Journal of Microbiology and Immunology
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European Journal of Microbiology and Immunology
Language English
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