Authors:
Trudy M. Wassenaar Molecular Microbiology and Genomics Consultants, Germany

Search for other papers by Trudy M. Wassenaar in
Current site
Google Scholar
PubMed
Close
,
Anke Zschüttig

Search for other papers by Anke Zschüttig in
Current site
Google Scholar
PubMed
Close
,
Claudia Beimfohr vermicon AG, Germany

Search for other papers by Claudia Beimfohr in
Current site
Google Scholar
PubMed
Close
,
Thomas Geske Berlin, Germany

Search for other papers by Thomas Geske in
Current site
Google Scholar
PubMed
Close
,
Christian Auerbach Institute of Medical Microbiology and Hygiene, Germany

Search for other papers by Christian Auerbach in
Current site
Google Scholar
PubMed
Close
,
Helen Cook Center for Biological Sequence Analysis, Department of Systems Biology, Denmark

Search for other papers by Helen Cook in
Current site
Google Scholar
PubMed
Close
,
Kurt Zimmermann SymbioPharm GmbH, Germany

Search for other papers by Kurt Zimmermann in
Current site
Google Scholar
PubMed
Close
, and
Florian Gunzer Institute of Medical Microbiology and Hygiene, Germany

Search for other papers by Florian Gunzer in
Current site
Google Scholar
PubMed
Close
Open access

The probiotic product Symbioflor2 (DSM 17252) is a bacterial concentrate of six different Escherichia coli genotypes, whose complete genome sequences are compared here, between each other as well as to other E. coli genomes. The genome sequences of Symbioflor2 E. coli components contained a number of virulence-associated genes. Their presence seems to be in conflict with a recorded history of safe use, and with the observed low frequency of adverse effects over a period of more than 6 years. The genome sequences were used to identify unique sequences for each component, for which strain-specific hybridization probes were designed. A colonization study was conducted whereby five volunteers were exposed to an exceptionally high single dose. The results showed that the probiotic E. coli could be detected for 3 months or longer in their stools, and this was in particular the case for those components containing higher numbers of virulence-associated genes. Adverse effects from this long-term colonization were absent. Thus, the presence of the identified virulence genes does not result in a pathogenic phenotype in the genetic background of these probiotic E. coli.

  • Littlejohn TG, DiBerardino D, Messerotti LJ, Spiers SJ, Skurray RA: Structure and evolution of a family of genes encoding antiseptic and disinfectant resistance in Staphylococcus aureus. Gene 101, 59–66 (1991)

  • Wassenaar TM, Gaastra W: Bacterial virulence, where to draw the line? FEMS Microbiol Letters 201, 1–7 (2001)

  • Wassenaar TM, Alter T (2012): Virulence genes in microbial risk assessment of probiotic organisms: what do genome sequences tell us? In: Beneficial microorganisms in agriculture, food and the environment, eds. Sundh I, Goettel M, Wilckes A, CABI, pp. 180–196

  • Wassenaar TM, Bohlin J, Binnewies TT, Ussery DW: Genome comparison of bacterial pathogens. Genome Dyn 6, 1–20 (2009)

  • Willenbrock H, Hallin PF, Wassenaar TM, Ussery DW: Characterization of probiotic Escherichia coli isolates with a novel pan-genome microarray. Genome Biol 8, R267 (2007)

  • Sonnenborn U, Schulze J: The non-pathogenic Escherichia coli strain Nissle 1917 — features of a versatile probiotic. Microb Ecol Health Dis 21, 122–158 (2009)

  • Grozdanov L, Raasch C, Schulze J, Sonnenborn U, Gottschalk G, Hacker, J Dobrindt U: Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917. J Bacteriol 186, 5432–5441 (2004)

  • Hancock V, Vejborg RM, Klemm P: Functional genomics of probiotic Escherichia coli Nissle 1917 and 83972, and UPEC strain CFT073: comparison of transcriptomes, growth and biofilm formation. Mol Genet Genomics 284, 437–454 (2010)

  • Lukjancenko O, Wassenaar TM, Ussery DW: Comparison of 61 sequenced Escherichia coli genomes. Microb Ecol 60, 708–720 (2010)

  • Zschüttig A, Auerbach C, Meltke S, Eichhorn C, Brandt M, Blom J, Goesmann A, Jarek M, Scharfe M, Zimmermann K, Wassenaar TM, and Gunzer F: Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and draft sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8. Submitted to Genome Announcement

  • Vesth T, Lagesen K, Acar O, Ussery D: CMG-Biotools, a Free Workbench for Basic Comparative Microbial Genomics. PLoS One 8, e60120 (2013)

  • Snipen L, Ussery DW: Standard operating procedure for computing pangenome trees. Stand Genomic Sci 2, 135–141 (2010)

  • Zhou CE, Smith J, Lam M, Zemla A, Dyer MD, Slezak T: MvirDB-a microbial database of protein toxins, virulence factors and antibiotic resistance genes for bio-defence applications. Nucl Acids Res 35 (Database issue), D391–D394 (2006)

  • Chen LH, Xiong ZH, Sun LL, Yang J and Jin Q: VFDB 2012 update: toward the genetic diversity and molecular evolution of bacterial virulence factors. Nucleic Acids Res 40 (Database issue), D641–D645 (2012)

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 215, 403–410 (1990)

  • Elvers KT, Helps CR, Wassenaar TM, Allen VM, Newell DG: Development of a strain-specific molecular method for quantitating individual campylobacter strains in mixed populations. Appl Environ Microbiol 74, 2321–2331 (2008)

  • Zschüttig A, Zimmermann K, Blom J, Goesmann A, Pöhlmann C, Gunzer F: Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10. PLoS One 7, e33351 (2012)

  • Maiden MC, Bygraves JA, Feil E, Morelli G, Russell JE, Urwin R, Zhang Q, Zhou J, Zurth K, Caugant DA, Feavers IM, Achtman M, Spratt BG: Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci USA 95, 3140–3145 (1998)

  • Monteiro C, Saxena I, Wang X, Kader A, Bokranz W, Simm R, Nobles D, Chromek M, Brauner A, Brown RM Jr., Römling U: Characterization of cellulose production in Escherichia coli Nissle 1917 and its biological consequences. Environ Microbiol 11, 1105–1116 (2009)

  • Linke D, Riess T, Autenrieth IB, Lupas A, Kempf VA: Trimeric autotransporter adhesins: variable structure, common function. Trends Microbiol 14, 264–270 (2006)

  • Al-Hasani K, Henderson IR, Sakellaris H, Rajakumar K, Grant T, Nataro JP, Robins-Browne R, Adler B: The sigA gene which is borne on the she pathogenicity island of Shigella flexneri 2a encodes an exported cytopathic protease involved in intestinal fluid accumulation. Infect Immun 68, 2457–2463 (2000)

  • Bücker R, Schulz E, Günzel D, Bojarski C, Lee IF, John LJ, Wiegand S, Janßen T, Wieler LH, Dobrindt U, Beutin L, Ewers C, Fromm M, Siegmund B, Troeger H, Schulzke JD: a-Haemolysin of Escherichia coli in IBD: a potentiator of inflammatory activity in the colon. Gut 63, 1893–1901 (2014)

  • Mahan MJ, Heithoff DM, Sinsheimer RL, Low DA: Assessment of bacterial pathogenesis by analysis of gene expression in the host. Annu Rev Genet 34, 139–164 (2000)

  • Collapse
  • Expand
The author instruction is available in PDF.
Please, download the file from HERE.
 

Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Editorial Board

  • Berit Bangoura (University of Wyoming, USA)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Magdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Edit Buzas (Semmelweis University, Hungary)
  • Renato Damatta (UENF, Brazil)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Achim Kaasch (Otto von Guericke University Magdeburg, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Susanne A. Wolf (MDC-Berlin, Germany)

 

Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildiko.dunay@med.ovgu.de

Indexing and Abstracting Services:

  • PubMed Central
  • Scopus
  • ESCI
  • CABI
  • CABELLS Journalytics

 

2022  
Web of Science  
Total Cites
WoS
717
Journal Impact Factor 2.2
Rank by Impact Factor

n/a

Impact Factor
without
Journal Self Cites
2.2
5 Year
Impact Factor
2.8
Journal Citation Indicator 0.66
Rank by Journal Citation Indicator

Microbiology (Q2)

Scimago  
Scimago
H-index
11
Scimago
Journal Rank
0.614
Scimago Quartile Score Microbiology (Q3)
Microbiology (medical) (Q3)
Immunology and Allergy (Q3)
Immunology (Q3)
Scopus  
Scopus
Cite Score
8.3
Scopus
CIte Score Rank
Microbiology 33/163 (80th PCTL)
Microbiology (medical) 28/124 (77th PCTL)
Immunology and Allergy 63/211 (70th PCTL)
Immunology 69/217 (68th PCTL)
Scopus
SNIP
1.221

 

2021  
Web of Science  
Total Cites
WoS
790
Journal Impact Factor not applicable
Rank by Impact Factor not applicable
Impact Factor
without
Journal Self Cites
not applicable
5 Year
Impact Factor
not applicable
Journal Citation Indicator 0,64
Rank by Journal Citation Indicator Microbiology 81/157
Scimago  
Scimago
H-index
not indexed
Scimago
Journal Rank
not indexed
Scimago Quartile Score not indexed
Scopus  
Scopus
Cite Score
not indexed
Scopus
CIte Score Rank
  not indexed
Scopus
SNIP
not indexed

2020  
CrossRef Documents 23
WoS Cites 708
Wos H-index 27
Days from submission to acceptance 219
Days from acceptance to publication 176
Acceptance Rate 70%

2019  
WoS
Cites
558
CrossRef
Documents
24
Acceptance
Rate
92%

 

European Journal of Microbiology and Immunology
Publication Model Gold Open Access
Submission Fee none
Article Processing Charge 600 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

European Journal of Microbiology and Immunology
Language English
Size A4
Year of
Foundation
2011
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 2062-509X (Print)
ISSN 2062-8633 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2023 0 24 13
Dec 2023 0 85 17
Jan 2024 0 80 11
Feb 2024 0 67 20
Mar 2024 0 37 10
Apr 2024 0 17 3
May 2024 0 0 0