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  • 1 Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
  • | 2 Hôpitaux Universitaires Pitié-Salpétrière — Charles Foix, Paris, France
  • | 3 Charité Medical School, Berlin, Germany
Open access

Detection of IgG and IgM antibodies is commonly performed for the diagnosis of infection with Toxoplasma gondii. We determined the accuracy of the Elecsys Toxo IgG and IgM test at four European laboratories compared to local reference methods. Coefficients of variation for reproducibility ranged from 1.0 to 6.5% for IgG and from 0.8 to 3.2% for IgM. Seroconversion panels revealed high overall concordance with the reference tests. The Elecsys test detected IgG antibodies earlier than the Cobas Core IgG test in 19 of 47 panels; persisting IgM antibodies were observed in the VIDAS but not the Elecsys test in five of 47 panels. In 31.4% of latent stage sera with persistent IgM antibodies (positive LIASON IgM), the Elecsys IgM test gave negative results indicating increased “clinical” specificity. Sensitivity and specificity of the Elecsys IgG assay ranged from 99.45 to 100% and 87.50–99.80%, respectively, and 91.11–95.74 and 98.45–99.79% for the Elecsys IgM assay, respectively.

In conclusion, excellent reproducibility and accuracy make the Elecsys Toxo G and M tests highly suitable for the detection of anti-T. gondii IgG and IgM antibodies. The lower detection rates for persistent IgM in the Elecsys IgM test increase “clinical” specificity and decrease the need for follow-up testing.

  • 1.

    Montoya JG , Liesenfeld O: Toxoplasmosis. Lancet 363, 19651976 (2004)

  • 2.

    Maenz M , Schluter D, Liesenfeld O, Schares G, Gross U, et al. : Ocular toxoplasmosis past, present and new aspects of an old disease. Prog Retin Eye Res 39, 77106 (2014)

    • Search Google Scholar
    • Export Citation
  • 3.

    Gras L , Wallon M, Pollak A, Cortina-Borja M, Evengard B, et al. : Association between prenatal treatment and clinical manifestations of congenital toxoplasmosis in infancy: a cohort study in 13 European centres. Acta Paediatr 94, 17211731 (2005)

    • Search Google Scholar
    • Export Citation
  • 4.

    Kieffer F , Wallon M, Garcia P, Thulliez P, Peyron F, et al. : Risk factors for retinochoroiditis during the first 2 years of life in infants with treated congenital toxoplasmosis. Pediatr Infect Dis J 27, 2732 (2008)

    • Search Google Scholar
    • Export Citation
  • 5.

    Robert-Gangneux F , Darde ML: Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev 25, 264296 (2012)

  • 6.

    Murat JB , Dard C, Fricker Hidalgo H, Darde ML, Brenier-Pinchart MP, et al. : Comparison of the VIDAS system and two recent fully automated assays for diagnosis and followup of toxoplasmosis in pregnant women and newborns. Clin Vaccine Immunol 20, 12031212 (2013)

    • Search Google Scholar
    • Export Citation
  • 7.

    Prusa AR , Hayde M, Unterasinger L, Pollak A, Herkner KR, et al. : Evaluation of the Roche Elecsys Toxo IgG and IgM electrochemiluminescence immunoassay for the detection of gestational Toxoplasma infection. Diagn Microbiol Infect Dis 68, 352357 (2010)

    • Search Google Scholar
    • Export Citation
  • 8.

    Van Helden J : Performance of Elecsys toxo IgG and IgM immunoassays. Clin Lab 55, 267273 (2009)

  • 9.

    Lesle F , Touafek F, Fekkar A, Mazier D, Paris L: Discrepancies between a new highly sensitive Toxoplasma gondii ELISA assay and other reagents: interest of Toxo IgG Western blot. Eur J Clin Microbiol Infect Dis 30, 12071212 (2011)

    • Search Google Scholar
    • Export Citation
  • 10.

    Reiter-Owona I , Petersen E, Joynson D, Aspock H, Darde ML, et al. : The past and present role of the Sabin-Feldman dye test in the serodiagnosis of toxoplasmosis. Bull World Health Organ 77, 929935 (1999)

    • Search Google Scholar
    • Export Citation
  • 11.

    Dannemann BR , Vaughan WC, Thulliez P, Remington JS: Differential agglutination test for diagnosis of recently acquired infection with Toxoplasma gondii. J Clin Microbiol 28, 19281933 (1990)

    • Search Google Scholar
    • Export Citation
  • 12.

    Montoya JG , Remington JS: Management of Toxoplasma gondii infection during pregnancy. Clin Infect Dis 47, 554566 (2008)

  • 13.

    Boubaker K , Raeber PA, Vaudaux B, Bucher HC, Garweg JG, et al. : Toxoplasmosis during pregnancy and infancy. A new approach for Switzerland. Swiss Med Wkly 138, 18 (2008)

    • Search Google Scholar
    • Export Citation
  • 14.

    Jost C , Touafek F, Fekkar A, Courtin R, Ribeiro M, et al. : Utility of immunoblotting for early diagnosis of toxoplasmosis seroconversion in pregnant women. Clin Vaccine Immunol 18, 19081912 (2011)

    • Search Google Scholar
    • Export Citation
  • 15.

    Kohler S , Rossler D, Hornauer S, Upmeier B, Franck J, et al. : Neutralization assay to resolve discrepancies between positive results in new highly sensitive anti-Toxoplasmagondii IgG assays and negative results in reference tests. Eur J Clin Microbiol Infect Dis 29, 359363 (2010)

    • Search Google Scholar
    • Export Citation
  • 16.

    Liesenfeld O , Press C, Flanders R, Ramirez R, Remington JS: Study of Abbott Toxo IMx system for detection of immunoglobulin G and immunoglobulin M toxoplasma antibodies: value of confirmatory testing for diagnosis of acute toxoplasmosis. J Clin Microbiol 34, 25262530 (1996)

    • Search Google Scholar
    • Export Citation
  • 17.

    Liesenfeld O , Press C, Montoya JG, Gill R, Isaac-Renton JL, et al. : False-positive results in immunoglobulin M (IgM) toxoplasma antibody tests and importance of confirmatory testing: the Platelia Toxo IgM test. J Clin Microbiol 35, 174178 (1997)

    • Search Google Scholar
    • Export Citation
  • 18.

    Del Bono V , Canessa A, Bruzzi P, Fiorelli MA, Terragna A: Significance of specific immunoglobulin M in the chronological diagnosis of 38 cases of toxoplasmic lymphadenopathy. J Clin Microbiol 27, 21332135 (1989)

    • Search Google Scholar
    • Export Citation
  • 19.

    Bobic B , Sibalic D, Djurkovic-Djakovic O: High levels of IgM antibodies specific for Toxoplasma gondii in pregnancy 12 years after primary toxoplasma infection. Case report. Gynecol Obstet Invest 31, 182184 (1991)

    • Search Google Scholar
    • Export Citation
  • 20.

    Gras L , Gilbert RE, Wallon M, Peyron F, Cortina-Borja M: Duration of the IgM response in women acquiring Toxoplasmagondii during pregnancy: implications for clinical practice and cross-sectional incidence studies. Epidemiol Infect 132, 541548 (2004)

    • Search Google Scholar
    • Export Citation
  • 21.

    Liesenfeld O , Montoya JG, Tathineni NJ, Davis M, Brown BW, Jr., et al. : Confirmatory serologic testing for acute toxoplasmosis and rate of induced abortions among women reported to have positive Toxoplasma immunoglobulin M antibody titers. Am J Obstet Gynecol 184, 140145 (2001)

    • Search Google Scholar
    • Export Citation
  • 22.

    Petersen E , Borobio MV, Guy E, Liesenfeld O, Meroni V, et al. : European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasmagondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index. J Clin Microbiol 43, 15701574 (2005)

    • Search Google Scholar
    • Export Citation
  • 23.

    Gilbert RE , Thalib L, Tan HK, Paul M, Wallon M, et al. : Screening for congenital toxoplasmosis: accuracy of immunoglobulin M and immunoglobulin A tests after birth. J Med Screen 14, 813 (2007)

    • Search Google Scholar
    • Export Citation
  • 24.

    Murat JB , L’Ollivier C, Fricker Hidalgo H, Franck J, Pelloux H, et al. : Evaluation of the new Elecsys Toxo IgG avidity assay for toxoplasmosis and new insights into the interpretation of avidity results. Clin Vaccine Immunol 19, 18381843 (2012)

    • Search Google Scholar
    • Export Citation
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