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University Medicine Rostock, Germany
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We compared the performance of an in-house and a commercial malaria polymerase chain reaction (PCR) assay using freeze–thawed hemolytic blood samples.
A total of 116 freeze–thawed ethylenediamine tetraacetic acid (EDTA) blood samples of patients with suspicion of malaria were analyzed by an in-house as well as by a commercially available real-time PCR.
Concordant malaria negative PCR results were reported for 39 samples and malaria-positive PCR results for 67 samples. The inhouse assay further detected one case of Plasmodium falciparum infection, which was negative in the commercial assay as well as five cases of P. falciparum malaria and three cases of Plasmodium vivax malaria, which showed sample inhibition in the commercial assay. The commercial malaria assay was positive in spite of a negative in-house PCR result in one case. In all concordant results, cycle threshold values of P. falciparum-positive samples were lower in the commercial PCR than in the in-house assay.
Although Ct values of the commercial PCR kit suggest higher sensitivity in case of concordant results, it is prone to inhibition if it is applied to hemolytic freeze–thawed blood samples. The number of misidentifications was, however, identical for both real-time PCR assays.
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Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildiko.dunay@med.ovgu.de
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| 2024 | |
|---|---|
| Scopus | |
| CiteScore | |
| CiteScore rank | |
| SNIP | |
| Scimago | |
| SJR index | 0.569 |
| SJR Q rank | Q3 |
| 2023 | |
|---|---|
| Web of Science | |
| Total Cites WoS |
674 |
| Journal Impact Factor | 3.3 |
| Rank by Impact Factor |
Q2 |
| Impact Factor without Journal Self Cites |
3.1 |
| 5 Year Impact Factor |
3.2 |
| Scimago | |
| Scimago H-index |
15 |
| Scimago Journal Rank |
0.601 |
| Scimago Quartile Score | Microbiology (medical) (Q2) Microbiology (Q3) Immunology and Allergy (Q3) Immunology (Q3) |
| Scopus | |
| Scopus Cite Score |
5.0 |
| Scopus CIte Score Rank |
Microbiology (medical) Q2 |
| Scopus SNIP |
0.832 |
| European Journal of Microbiology and Immunology | |
|---|---|
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| European Journal of Microbiology and Immunology | |
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2011 |
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