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  • 1 German Armed Forces Hospital of Hamburg, Germany
  • 2 Bernhard Nocht Institute for Tropical Medicine Hamburg, Germany
  • 3 University Medicine Rostock, Germany
Open access

We compared the performance of an in-house and a commercial malaria polymerase chain reaction (PCR) assay using freeze–thawed hemolytic blood samples.

A total of 116 freeze–thawed ethylenediamine tetraacetic acid (EDTA) blood samples of patients with suspicion of malaria were analyzed by an in-house as well as by a commercially available real-time PCR.

Concordant malaria negative PCR results were reported for 39 samples and malaria-positive PCR results for 67 samples. The inhouse assay further detected one case of Plasmodium falciparum infection, which was negative in the commercial assay as well as five cases of P. falciparum malaria and three cases of Plasmodium vivax malaria, which showed sample inhibition in the commercial assay. The commercial malaria assay was positive in spite of a negative in-house PCR result in one case. In all concordant results, cycle threshold values of P. falciparum-positive samples were lower in the commercial PCR than in the in-house assay.

Although Ct values of the commercial PCR kit suggest higher sensitivity in case of concordant results, it is prone to inhibition if it is applied to hemolytic freeze–thawed blood samples. The number of misidentifications was, however, identical for both real-time PCR assays.

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  • 1.

    Frickmann H , Schwarz NG, Holtherm HU, Maaßen W, Vorderwülbecke F, Erkens K, Fischer M, Morwinsky T, Hagen RM: Compliance with antimalarial chemoprophylaxis in German soldiers: a 6-year survey. Infection 41, 311320 (2013)

    • Search Google Scholar
    • Export Citation
  • 2.

    Abba K , Kirkham AJ, Olliaro PL, Deeks JJ, Donegan S, Garner P, Takwoingi Y: Rapid diagnostic tests for diagnosing uncomplicated non-falciparum or Plasmodium vivax malaria in endemic countries. Cochrane Database Syst Rev 12, CD011431 (2014)

    • Search Google Scholar
    • Export Citation
  • 3.

    Gillet P , Mori M, Van Esbroeck M, Van den Ende J, Jacobs J: Assessment of the prozone effect in malaria rapid diagnostic tests. Malar J 8, 271 (2009)

    • Search Google Scholar
    • Export Citation
  • 4.

    Gillet P , Scheirlinck A, Stokx J, De Weggheleire A, Chaúque HS, Canhanga OD, Tadeu BT, Mosse CD, Tiago A, Mabunda S, Bruggeman C, Bottieau E, Jacobs J: Prozone in malaria rapid diagnostics tests: how many cases are missed? Malar J 10, 166 (2011)

    • Search Google Scholar
    • Export Citation
  • 5.

    Mahajan B , Zheng H, Pham PT, Sedegah MY, Majam VF, Akolkar N, Rios M, Ankrah I, Madjitey P, Amoah G, Addison E, Quakyi IA, Kumar S: Polymerase chain reactionbased tests for pan-species and species-specific detection of human Plasmodium parasites. Transfusion 52, 19491956 (2012)

    • Search Google Scholar
    • Export Citation
  • 6.

    Frickmann H , Hinz R, Hagen RM: Comparison of an automated nucleic acid extraction system with the columnbased procedure. Eur J Microbiol Immunol (Bp) 5, 94102 (2015)

    • Search Google Scholar
    • Export Citation
  • 7.

    Mangold KA , Manson RU, Koay ES, Stephens L, Regner M, Thomson RB Jr., Peterson LR, Kaul KL: Real-time PCR for detection and identification of Plasmodium spp. J Clin Microbiol 43, 24352440 (2005)

    • Search Google Scholar
    • Export Citation
  • 8.

    Berry A , Benoit-Vical F, Fabre R, Cassaing S, Magnaval JF: PCR-based methods to the diagnosis of imported malaria. Parasite 15, 484488 (2008)

    • Search Google Scholar
    • Export Citation
  • 9.

    Berry A , Fabre R, Benoit-Vical F, Cassaing S, Magnaval JF: Contribution of PCR-based methods to diagnosis and management of imported malaria. Med Trop (Mars) 65, 176183 (2005)

    • Search Google Scholar
    • Export Citation
  • 10.

    Hawkes M , Kain KC: Advances in malaria diagnosis. Expert Rev Anti Infect Ther 5, 485495 (2007)

  • 11.

    Hänscheid T , Grobusch MP: How useful is PCR in the diagnosis of malaria? Trends Parasitol 18, 395398 (2002)

  • 12.

    Scholl PF , Kongkasuriyachai D, Demirev PA, Feldman AB, Lin JS, Sullivan DJ Jr., Kumar N: Rapid detection of malaria infection in vivo by laser desorption mass spectrometry. Am J Trop Med Hyg 71, 546551 (2004)

    • Search Google Scholar
    • Export Citation
  • 13.

    Nyunt M , Pisciotta J, Feldman AB, Thuma P, Scholl PF, Demirev PA, Lin JS, Shi L, Kumar N, Sullivan DJ Jr.: Detection of Plasmodium falciparum in pregnancy by laser desorption mass spectrometry. Am J Trop Med Hyg 73, 485490 (2005)

    • Search Google Scholar
    • Export Citation
  • 14.

    Gascoyne P , Satayavivad J, Ruchirawat M: Microfluidic approaches to malaria detection. Acta Trop 89, 357369 (2004)

  • 15.

    Oriero EC , Jacobs J, Van Geertruyden JP, Nwakanma D, D’Alessandro U: Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination. J Antimicrob Chemother 70, 213 (2015)

    • Search Google Scholar
    • Export Citation

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Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Vice Editor(s)-in-Chief: Fuchs, Anja

Editorial Board

Chair of the Editorial Board:
Jeffrey S. Buguliskis (Thomas Jefferson University, USA)

  • Jörn Albring (University of Münster, Germany)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Megdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Jeff Buguliskis (Thomas Jefferson University, USA)
  • Edit Buzas (Semmelweis University, Hungary)
  • Charles Collyer (University of Sydney, Australia)
  • Renato Damatta (UENF, Brazil)
  • Ivelina Damjanova (Semmelweis University, Hungary)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Anna Erdei (Eötvös Loránd University, Hungary)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Beniam Ghebremedhin (Witten/Herdecke University, Germany)
  • Nancy Guillen (Institute Pasteur, France)
  • Georgina L. Hold (University of Aberdeen, United Kingdom)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Zsuzsanna Izsvak (MDC-Berlin, Germany)
  • Achim Kaasch (University of Cologne, Germany)
  • Tamás Laskay (University of Lübeck, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Shreemanta Parida (Vaccine Grand Challenge Program, India)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Mary Jo Wick (University of Gothenburg, Sweden)
  • Susanne A. Wolf (MDC-Berlin, Germany)


Dr. Dunay, Ildiko Rita
Magdeburg, Germany
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