View More View Less
  • 1 German Armed Forces Hospital of Hamburg, Berlin, Germany
  • | 2 University Medicine Rostock, Berlin, Germany
Open access

ESBL (extended-spectrum-β-lactamase)-positive Enterobacteriaceae, which colonized European soldiers in tropical Western African Mali, were subjected to a molecular assessment of their resistance determinants. By doing so, a better insight into the locally endemic pattern of ESBL-associated β-lactamase genes was aspired.

From a previous study on diarrhea in European soldiers on deployment in tropical Mali, 15 ESBL-positive Escherichia coli with demonstrated high clonal diversity and one positive Klebsiella pneumoniae were assessed. Polymerase chain reactions (PCRs) for blaTEM and blaSHV β-lactamase genes with subsequent sequencing for the discrimination of ESBL- and non-ESBL variants were performed, followed by four group-specific PCRs for blaCTX-M genes.

Non-ESBL-associated blaTEM-1 was identified in six out of 15 (40%) E. coli strains, while 100% of the assessed strains were positive for group I blaCTX-M.

Considering the known clonal diversity of the assessed strains, the striking restriction to one group of blaCTX-M genes accounting for the ESBL phenotypes of the isolates suggests little genetic exchange in the local setting. Under such circumstances of restricted numbers of locally endemic target genes, PCR-based screening approaches for ESBL colonization might be promising.

  • 1.

    Kantele A , Lääveri T, Mero S, Vilkman K, Pakkanen SH, Ollgren J, Antikainen J, Kirveskari J: Antimicrobials increase travelers’ risk of colonization by extended-spectrum betalactamase-producing Enterobacteriaceae. Clin Infect Dis 60, 837846 (2015)

    • Search Google Scholar
    • Export Citation
  • 2.

    Lübbert C , Straube L, Stein C, Makarewicz O, Schubert S, Mössner J, Pletz MW, Rodloff AC: Colonization with extended-spectrum beta-lactamase-producing and carbapenemase-producing Enterobacteriaceae in international travelers returning to Germany. Int J Med Microbiol 305, 148156 (2015)

    • Search Google Scholar
    • Export Citation
  • 3.

    Paltansing S , Vlot JA, Kraakman ME, Mesman R, Bruijning ML, Bernards AT, Visser LG, Veldkamp KE: Extended-spectrum ß-lactamase-producing Enterobacteriaceae among travelers from the Netherlands. Emerg Infect Dis 19, 12061213 (2013)

    • Search Google Scholar
    • Export Citation
  • 4.

    Peirano G , Laupland KB, Gregson DB, Pitout JD: Colonization of returning travelers with CTX-M-producing Escherichia coli. J Travel Med 18, 299303 (2011)

    • Search Google Scholar
    • Export Citation
  • 5.

    Frickmann H , Warnke P, Frey C, Schmidt S, Janke C, Erkens K, Schotte U, Köller T, Maaßen W, Podbielski A, Binder A, Hinz R, Queyriaux B, Wiemer D, Schwarz NG, Hagen RM: Surveillance of food-and smear-transmitted pathogens in European Soldiers with diarrhea on deployment in the tropics: experience from the European Union Training Mission (EUTM) Mali. BioMed Res Int 2015, 573904 (2015)

    • Search Google Scholar
    • Export Citation
  • 6.

    Cullik A , Pfeifer Y, Prager R, von Baum H, Witte W: A novel IS26 structure surrounds blaCTX-M genes in different plasmids from German clinical Escherichia coli isolates. J Med Microbiol 59, 580587 (2010)

    • Search Google Scholar
    • Export Citation
  • 7.

    Hovhannisyan G , Adam T, Göbel U: Molekulare epidemiologie der ESBL-bildenden Escherichia coli-und Klebsiella spp.-isolate an einem universitätsklinikum. Mikrobiologie 25:510 (2015)

    • Search Google Scholar
    • Export Citation
  • 8.

    Pitout JD , Hossain A, Hanson ND: Phenotypic and molecular detection of CTX-M-beta-lactamases produced by Escherichia coli and Klebsiella spp. J Clin Microbiol 42, 57155721 (2014)

    • Search Google Scholar
    • Export Citation
  • 9.

    Bonnet R : Growing group of extended-spectrum betalactamases: the CTX-M enzymes. Antimicrob Agents Chemother 48, 114 (2004)

  • 10.

    Tandé D , Boisramé-Gastrin S, Münck MR, Héry-Arnaud G, Gouriou S, Jallot N, Nordmann P, Naas T: Intrafamilial transmission of extended-spectrum-ß-lactamase-producing Escherichia coli and Salmonella enterica Babelsberg among the families of internationally adopted children. J Antimicrob Chemother 65, 859865 (2010)

    • Search Google Scholar
    • Export Citation
  • 11.

    Obeng-Nkrumah N , Twum-Danso K, Krogfelt KA, Newman MJ: High levels of extended-spectrum beta-lactamases in a major teaching hospital in Ghana: the need for regular monitoring and evaluation of antibiotic resistance. Am J Trop Med Hyg 89, 960964 (2013)

    • Search Google Scholar
    • Export Citation
  • 12.

    Feglo P , Adu-Sarkodie Y, Ayisi L, Jain R, Spurbeck RR, Springman AC, Engleberg NC, Newton DW, Xi C, Walk ST. Emergence of a novel extended-spectrum-ß-lactamase (ESBL)-producing, fluoroquinolone-resistant clone of extraintestinal pathogenic Escherichia coli in Kumasi, Ghana. J Clin Microbiol 51, 728730 (2013)

    • Search Google Scholar
    • Export Citation
  • 13.

    Micheel V , Hogan B, Rakotoarivelo RA, Rakotozandrindrainy R, Razafimanatsoa F, Razafindrabe T, Rakotondrainiarivelo JP, Crusius S, Poppert S, Schwarz NG, May J, Frickmann H, Hagen RM: Identification of nasal colonization with ß-lactamase-producing Enterobacteriaceae in patients, health care workers and students in Madagascar. Eur J Microbiol Immunol (Bp) 5, 116125 (2015)

    • Search Google Scholar
    • Export Citation
The author instruction is available in PDF.
Please, download the file from HERE.

Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Vice Editor(s)-in-Chief: Fuchs, Anja

Editorial Board

Chair of the Editorial Board:
Jeffrey S. Buguliskis (Thomas Jefferson University, USA)

  • Jörn Albring (University of Münster, Germany)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Megdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Jeff Buguliskis (Thomas Jefferson University, USA)
  • Edit Buzas (Semmelweis University, Hungary)
  • Charles Collyer (University of Sydney, Australia)
  • Renato Damatta (UENF, Brazil)
  • Ivelina Damjanova (Semmelweis University, Hungary)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Anna Erdei (Eötvös Loránd University, Hungary)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Beniam Ghebremedhin (Witten/Herdecke University, Germany)
  • Nancy Guillen (Institute Pasteur, France)
  • Georgina L. Hold (University of Aberdeen, United Kingdom)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Zsuzsanna Izsvak (MDC-Berlin, Germany)
  • Achim Kaasch (University of Cologne, Germany)
  • Tamás Laskay (University of Lübeck, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Shreemanta Parida (Vaccine Grand Challenge Program, India)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Mary Jo Wick (University of Gothenburg, Sweden)
  • Susanne A. Wolf (MDC-Berlin, Germany)


Dr. Dunay, Ildiko Rita
Magdeburg, Germany

Indexing and Abstracting Services:

  • PubMed Central
  • Scopus
  • ESCI
  • CABI


CrossRef Documents 23
WoS Cites 708
Wos H-index 27
Days from submission to acceptance 219
Days from acceptance to publication 176
Acceptance Rate 70%



European Journal of Microbiology and Immunology
Publication Model Gold Open Access
Submission Fee none
Article Processing Charge 928 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

European Journal of Microbiology and Immunology
Language English
Size A4
Year of
2021 Volume 11
per Year
per Year
Founder Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Chief Executive Officer, Akadémiai Kiadó
ISSN 2062-509X (Print)
ISSN 2062-8633 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
May 2021 0 0 0
Jun 2021 0 1 0
Jul 2021 0 3 0
Aug 2021 0 1 1
Sep 2021 0 1 1
Oct 2021 0 0 0
Nov 2021 0 0 0