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  • 1 Universidade Federal de São Paulo, Botucatu St., 740, São Paulo, SP, Brazil
  • | 2 Instituto de Medicina Tropical de São Paulo, Botucatu st., 822, São Paulo, SP, Brazil
  • | 3 Universidade Federal de São Paulo, Botucatu St., 740, São Paulo, SP, Brazil
  • | 4 Instituto de Medicina Tropical de São Paulo, Dr. Enéas de Carvalho Aguiar Av., 470, São Paulo, SP, Brazil
  • | 5 Instituto Oswaldo Cruz, Brasil Av, 4365, Rio de Janeiro, RJ, Brazil
Open access

HSP90B1 is a gene that codifies heat shock protein 108 (HSP108) that belongs to a group of proteins induced under stress situation, and it has close relation with the nervous system, especially in the retina. Toxoplasma gondii causes ocular toxoplasmosis that has been associated with a late manifestation of the congenital toxoplasmosis although experimental models show that morphological alterations are already present during embryological development. Here, we used 18 eyes of Gallus domesticus embryos in 7th and 20th embryonic days to establish a model of congenital ocular toxoplasmosis, experimentally infected in its fifth day correlating with HSP90B1 gene expression. Embryos’ eyes were histologically evaluated, and gene expression was performed by real-time polymerase chain reaction (PCR). Our data showed parasite present in the choroid, unusual migration of retinal pigment epithelium, and chorioretinal scars, and a tendency to a lower expression of the HSP90B1 gene upon experimental infection. This is a promising model to better understand T. gondii etiopathogeny.

  • 1.

    Tedesco RC , Vitor RWA, Brandão GP, Calabresi KS: Ocular toxoplasmosis signs in mice embryo. Micron 38, 729733 (2007)

  • 2.

    Lu F , Huang S, Kasper LH: The temperature-sensitive mutants of Toxoplasma gondii and ocular toxoplasmosis. Vaccine 27, 573580 (2009)

  • 3.

    Kim K , Weiss LM: Toxoplasma: the next 100 years. Microb Infect 10, 978984 (2008)

  • 4.

    Jones LA , Alexander J, Roberts CW: Ocular toxoplasmosis: in the storm of the eye. Parasite Immunol 28, 635642 (2006)

  • 5.

    Menzies FM , Henriquez FL, Roberts, CG: Immunological control of congenital toxoplasmosis in the murine model. Immunol Lett 115, 8389 (2008)

    • Search Google Scholar
    • Export Citation
  • 6.

    Robert-Gangneux F : It is not only the cat that did it: How to prevent and treat congenital toxoplasmosis. J Infect 68, 125133 (2014)

    • Search Google Scholar
    • Export Citation
  • 7.

    Fleige T , Fischer K, Ferguson DJP, Gross UWE, Bohne W: Carbohydrate metabolism in the Toxoplasma gondii apicoplast: Localization of three glycolytic isoenzymes, the single pyruvate dehydrogenase complex, and a plasticid phosphate translocator. Am Soc Microbiol 6, 984996 (2007)

    • Search Google Scholar
    • Export Citation
  • 8.

    Miguel ND , Echeverria PC, Angel SO: Differential subcellular localization of members of the Toxoplasma gondii small heat shock protein family. Eukaryot Cell 4, 19901997 (2005)

    • Search Google Scholar
    • Export Citation
  • 9.

    Howe DK , Honoré S, Derouin F, Sibley LD: Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis. J Clin Microbiol 35, 14111414 (1997)

    • Search Google Scholar
    • Export Citation
  • 10.

    Bollani L , Stroccio L, Stronati M: Congenital toxoplasmosis. Early Hum Dev 89, 7071 (2013)

  • 11.

    Gormley PD , Pavesio CE, Luthert P, Lightman S: Retinochoroiditis is induced by oral administration of Toxoplasma gondii cysts in the hamster model. Exp Eye Res 68, 657661 (1999)

    • Search Google Scholar
    • Export Citation
  • 12.

    Shin DH , Kim HJ, Kim J, Baer SR, Cho SS: Heat shock protein 108 mRNA expression during chicken retina development. Neurosci Lett 344, 2528 (2003)

    • Search Google Scholar
    • Export Citation
  • 13.

    Zhao J , Yoneda M, Inoue Y, Kakizaki H, Ohno-Jinno A, Kataoka T, Iwaki M, Zako M: Expression profile of heat shock protein 108 during retinal development in the chick. Neurosci Lett 297, 1014 (2006)

    • Search Google Scholar
    • Export Citation
  • 14.

    Sargan DR , Tsai MJ, O’Malley BW: HSP108, a novel heat shock inducible protein of chicken. Biochem 25, 65256258 (1986)

  • 15.

    Schlesinger MK : Heat shock proteins. J Biol Chem 265, 1211112114 (1990)

  • 16.

    Cho SS , Lucas JJ, Eun Jung YOO Yb Lee KH, Park, KH, Hwang DH, Baik SH: Distribution of transferrin binding protein immunoreactivity in the chicken central and peripheral nervous systems. J Comp Neurol 382, 260271 (1997)

    • Search Google Scholar
    • Export Citation
  • 17.

    Namazi F , Oryan A, Namavari MM, Rahimain A: Experimental infection of embryonated eggs of chicken with Besnoitia caprae. Trop Biomed 27, 413423 (2010)

    • Search Google Scholar
    • Export Citation
  • 18.

    Tedesco RC , Smith RL, Corte-Real S, Calabrese KS: Ocular toxoplasmosis in mice: comparison of two routes of infection. Parasitol 131, 303307 (2005)

    • Search Google Scholar
    • Export Citation
  • 19.

    Wen-Qiao F , Hong-Ning W, Yi Z, Zhong-Bin G, Ting W, Chang-Wen X, An-Yun Z, Xin Y: Comparative dynamic distribution of avian infectious bronchitis Virus M41, H120, and SAIBK strains by quantitative Real-Time RTPCR in SPF chickens. Biosci Biotechnol Biochem 276, 22552260 (2012)

    • Search Google Scholar
    • Export Citation
  • 20.

    Siegel S , Castelan NJ (1988): Nonparametric Statistics for Behavioral Sciences. Mc Graw-Hill, New York.

  • 21.

    Hamburger V , Hamilton HL: A series of normal stages in the development of the chick embryo. J Morphol 66, 4992 (1951)

  • 22.

    Mey J , Thanos S: Development of the visual system of the chick I. Cell differentiation and histogenesis. Brain Res Rev 32, 343379 (2000)

    • Search Google Scholar
    • Export Citation
  • 23.

    Garweg JG , Boehnke M: The antibody response in experimental ocular toxoplasmosis. Graef’s Arch Clin Exp Ophthalmol 244, 16681679 (2006)

    • Search Google Scholar
    • Export Citation
  • 24.

    Biancifiori F , Rondini C, Grelloni V, Frescura T: Avian toxoplasmosis: experimental infection of chicken and pigeon. Comp Immun Microbiol Infect Dis 9, 337346 (1986)

    • Search Google Scholar
    • Export Citation
  • 25.

    Commodaro A , Belfort RN, Rizzo LV, Muccioli C, Silveira C, Claudio JR, Burnier MNJR Belfor R: Ocular toxoplasmosis –an update and review of the literature. Mem Inst Oswaldo Cruz, 104, 345350 (2009)

    • Search Google Scholar
    • Export Citation
  • 26.

    Bok D , Young R, (1979): Phagocytic properties of the retinal pigment epithelium. In: The Retinal Pigment Epithelium, ed. Zinn KM, Marmar MF, Harvard University Press, Cambridge, pp. 148174.

    • Search Google Scholar
    • Export Citation
  • 27.

    Jones JL , Dubey JP: Waterborne toxoplasmosis: recent developments. Exp Parasitol 124, 1025 (2010).

  • 28.

    Chang Z , Ming L, Kim SS, Park JS: Potential role of HSP90 in mediating the interaction between estrogen receptor (ER) and aryl hydrocarbon receptor (AhR) signaling pathways. Toxicol Lett 226, 613 (2014)

    • Search Google Scholar
    • Export Citation
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Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Vice Editor(s)-in-Chief: Fuchs, Anja

Editorial Board

Chair of the Editorial Board:
Jeffrey S. Buguliskis (Thomas Jefferson University, USA)

  • Jörn Albring (University of Münster, Germany)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Megdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Jeff Buguliskis (Thomas Jefferson University, USA)
  • Edit Buzas (Semmelweis University, Hungary)
  • Charles Collyer (University of Sydney, Australia)
  • Renato Damatta (UENF, Brazil)
  • Ivelina Damjanova (Semmelweis University, Hungary)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Anna Erdei (Eötvös Loránd University, Hungary)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Beniam Ghebremedhin (Witten/Herdecke University, Germany)
  • Nancy Guillen (Institute Pasteur, France)
  • Georgina L. Hold (University of Aberdeen, United Kingdom)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Zsuzsanna Izsvak (MDC-Berlin, Germany)
  • Achim Kaasch (University of Cologne, Germany)
  • Tamás Laskay (University of Lübeck, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Shreemanta Parida (Vaccine Grand Challenge Program, India)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Mary Jo Wick (University of Gothenburg, Sweden)
  • Susanne A. Wolf (MDC-Berlin, Germany)


Dr. Dunay, Ildiko Rita
Magdeburg, Germany

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CrossRef Documents 23
WoS Cites 708
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Days from submission to acceptance 219
Days from acceptance to publication 176
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