Sporadic cases of gastroenteritis have been attributed to Arcobacter butzleri infection, but information about the underlying immunopathological mechanisms is scarce. We have recently shown that experimental A. butzleri infection induces intestinal, extraintestinal and systemic immune responses in gnotobiotic IL-10−/− mice. The aim of the present study was to investigate the immunopathological role of Toll-like Receptor-4, the receptor for lipopolysaccharide and lipooligosaccharide of Gram-negative bacteria, during murine A. butzleri infection. To address this, gnotobiotic IL-10−/− mice lacking TLR-4 were generated by broadspectrum antibiotic treatment and perorally infected with two different A. butzleri strains isolated from a patient (CCUG 30485) or fresh chicken meat (C1), respectively. Bacteria of either strain stably colonized the ilea of mice irrespective of their genotype at days 6 and 16 postinfection. As compared to IL-10−/− control animals, TLR-4−/− IL-10−/− mice were protected from A. butzleri-induced ileal apoptosis, from ileal influx of adaptive immune cells including T lymphocytes, regulatory T-cells and B lymphocytes, and from increased ileal IFN-γ secretion. Given that TLR-4-signaling is essential for A. butzleri-induced intestinal inflammation, we conclude that bacterial lipooligosaccharide or lipopolysaccharide compounds aggravate intestinal inflammation and may thus represent major virulence factors of Arcobacter. Future studies need to further unravel the molecular mechanisms of TLR-4-mediated A. butzleri-host interactions.
Supplementary Materials
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Ho HT , Lipman LJ, Gaastra W: Arcobacter, what is known and unknown about a potential foodborne zoonotic agent! Vet Microbiol 115(1–3),1–13 (2006)
-
2.
ICMSF ICoMSfF (2002). In: Microbiological Testing in Food Safety Management, 7, ed. Tompkin RB, Kluwer Academic/Plenum Publishers, New York, NY, p. 171.
-
3.
Ferreira S , Queiroz JA, Oleastro M, Domingues FC: Insights in the pathogenesis and resistance of Arcobacter: a review. Crit. Rev. Microbiol.1–20 (2015)
-
4.
Collado L , Figueras MJ: Taxonomy, epidemiology, and clinical relevance of the genus Arcobacter. Clin Microbiol Rev 24(1),174–192 (2011)
-
5.
Lappi V , Archer JR, Cebelinski E, Leano F, Besser JM, Klos RF, et al.: An outbreak of foodborne illness among attendees of a wedding reception in Wisconsin likely caused by Arcobacter butzleri. Foodborne Pathog Dis 10(3),250–255 (2013)
-
6.
Van den Abeele AM , Vogelaers D, Van Hende J, Houf K: Prevalence of Arcobacter species among humans, Belgium, 2008–2013. Emerg Infect Dis 20(10),1746–1749 (2014)
-
7.
Vandenberg O , Dediste A, Houf K, Ibekwem S, Souayah H, Cadranel S, et al.: Arcobacter species in humans. Emerg Infect Dis 10(10),1863–1867 (2004)
-
8.
Prouzet-Mauleon V , Labadi L, Bouges N, Menard A, Megraud F: Arcobacter butzleri: underestimated enteropathogen. Emerg Infect Dis 12(2),307–309 (2006)
-
9.
Fong TT , Mansfield LS, Wilson DL, Schwab DJ, Molloy SL, Rose JB: Massive microbiological groundwater contamination associated with a waterborne outbreak in Lake Erie, South Bass Island, Ohio. Environ Health Perspect 115(6),856–864 (2007)
-
10.
Karadas G , Sharbati S, Hanel I, Messelhausser U, Glocker E, Alter T, et al.: Presence of virulence genes, adhesion and invasion of Arcobacter butzleri. J Appl Microbiol 115(2),583–590 (2013)
-
11.
Levican A , Alkeskas A, Günter C, Forsyth SJ, Figueras MJ: The adherence and invasion of human intestinal cells by Arcobacter species and their virulence genotype. Appl Environ Microbiol 79(16),4951–4957 (2013)
-
12.
Karadas G , Bucker R, Sharbati S, Schulzke JD, Alter T, Golz G: Arcobacter butzleri isolates exhibit pathogenic potential in intestinal epithelial cell models. J Appl Microbiol. Oct 19. doi: 10.1111/jam.12979 [Epub ahead of print] (2015)
-
13.
Golla SC , Murano EA, Johnson LG, Tipton NC, Cureington EA, Savell JW: Determination of the occurrence of Arcobacter butzleri in beef and dairy cattle from Texas by various isolation methods. J Food Prot 65(12),1849–1853 (2002)
-
14.
Musmanno RA , Russi M, Lior H, Figura N: In vitro virulence factors of Arcobacter butzleri strains isolated from superficial water samples. New Microbiol 20(1),63–68 (1997).
-
15.
Carbone M , Maugeri TL, Giannone M, Gugliandolo C, Midiri A, Fera MT: Adherence of environmental Arcobacter butzleri and Vibrio spp. isolates to epithelial cells in vitro. Food Microbiol 20(5),611–616 (2003)
-
16.
Villarruel-Lopez A , Marquez-Gonzalez M, Garay-Martinez LE, Zepeda H, Castillo A, Mota de la Garza L, et al.: Isolation of Arcobacter spp. from retail meats and cytotoxic effects of isolates against vero cells. J Food Prot 66(8),1374–1378 (2003)
-
17.
Gugliandolo C , Irrera GP, Lentini V, Maugeri TL: Pathogenic Vibrio, Aeromonas and Arcobacter spp. associated with copepods in the Straits of Messina (Italy). Mar Pollut Bull 56(3),600–666 (2008)
-
18.
Bucker R , Troeger H, Kleer J, Fromm M, Schulzke JD: Arcobacter butzleri induces barrier dysfunction in intestinal HT-29/B6 cells. J Infect Dis 200(5),756–764 (2009)
-
19.
Haag LM , Fischer A, Otto B, Plickert R, Kuhl AA, Gobel UB, et al.: Campylobacter jejuni induces acute enterocolitis in gnotobiotic IL-10-/-mice via Toll-like-receptor-2 and -4 signaling. PLoS One 7(7), e40761(2012)
-
20.
Golz G , Karadas G, Alutis ME, Fischer A, Kuhl AA, Breithaupt A, et al.: Arcobacter butzleri Induce colonic, extraintestinal and systemic inflammatory responses in gnotobiotic IL-10 deficient mice in a strain-dependent manner. PLoS One 10(9), e0139402 (2015)
-
21.
Heimesaat MM , Karadas G, Alutis M, Fischer A, Kuhl AA, Breithaupt A, et al.: Survey of small intestinal and systemic immune responses following murine Arcobacter butzleri infection. Gut Pathog 7, 28 (2015)
-
22.
Janeway CA , Medzhitov R: Innate immune recognition. Annu Rev Immunol 20, 197–216 (2002)
-
23.
Vandamme P , Pugina P, Benzi G, Van Etterijck R, Vlaes L, Kersters K, et al.: Outbreak of recurrent abdominal cramps associated with Arcobacter butzleri in an Italian school. J Clin Microbiol 30(9),2335–2337 (1992).
-
24.
Heimesaat MM , Bereswill S, Fischer A, Fuchs D, Struck D, Niebergall J, et al.: Gram-negative bacteria aggravate murine small intestinal Th1-type immunopathology following oral infection with Toxoplasma gondii. J Immunol 177(12),8785–8795 (2006)
-
25.
Heimesaat MM , Lugert R, Fischer A, Alutis M, Kuhl AA, Zautner AE, et al.: Impact of Campylobacter jejuni cj0268c knockout mutation on intestinal colonization, translocation, and induction of immunopathology in gnotobiotic IL-10 deficient mice. PLoS One 9(2), e90148 (2014)
-
26.
Bereswill S , Fischer A, Plickert R, Haag LM, Otto B, Kuhl AA, et al.: Novel murine infection models provide deep insights into the “menage a trois” of Campylobacter jejuni, microbiota and host innate immunity. PLoS One 6(6), e20953 (2011)
-
27.
Madsen KL , Doyle JS, Jewell LD, Tavernini MM, Fedorak RN: Lactobacillus species prevents colitis in interleukin 10 gene-deficient mice. Gastroenterol 116(5),1107–1114 (1999).
-
28.
Heimesaat MM , Nogai A, Bereswill S, Plickert R, Fischer A, Loddenkemper C, et al.: MyD88/TLR9 mediated immunopathology and gut microbiota dynamics in a novel murine model of intestinal graft-versus-host disease. Gut 59(8),1079–1087 (2010)
-
29.
Haag LM , Fischer A, Otto B, Grundmann U, Kuhl AA, Gobel UB, et al.: Campylobacter jejuni infection of infant mice: acute enterocolitis is followed by asymptomatic intestinal and extra-intestinal immune responses. Eur J Microbiol Immunol (Bp) 2(1),2–11 (2012)
-
30.
Heimesaat MM , Haag LM, Fischer A, Otto B, Kuhl AA, Gobel UB, et al.: Survey of extra-intestinal immune responses in asymptomatic long-term Campylobacter jejuniinfected mice. Eur J Microbiol Immunol (Bp) 3(3),174–182 (2013)
-
31.
Otto B , Haag LM, Fischer A, Plickert R, Kuhl AA, Gobel UB, et al.: Campylobacter jejuni induces extra-intestinal immune responses via Toll-like-receptor-4 signaling in conventional IL-10 deficient mice with chronic colitis. Eur J Microbiol Immunol (Bp) 2(3),210–219 (2012)
-
32.
Silipo A , Sturiale L, Perino V, Garozzo D, Lanzetta R, Parrilli M, et al.: The structure of the carbohydrate backbone of the lipooligosaccharide from the halophilic bacterium Arcobacter halophilus. Carbohydr Res 345(6),850–853 (2010)
Monthly Content Usage
| Abstract Views | Full Text Views | PDF Downloads | |
|---|---|---|---|
| Aug 2020 | 0 | 2 | 2 |
| Sep 2020 | 0 | 8 | 2 |
| Oct 2020 | 0 | 7 | 3 |
| Nov 2020 | 0 | 5 | 5 |
| Dec 2020 | 0 | 4 | 3 |
| Jan 2021 | 0 | 2 | 1 |
| Feb 2021 | 0 | 0 | 0 |
Copyright Akadémiai Kiadó
AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.
Copyright Akadémiai Kiadó AKJournals is the trademark of Akadémiai Kiadó's journal publishing business branch.
Powered by PubFactory