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  • 1 Orchid Chemicals and Pharmaceuticals Ltd., 476/14, OMR, Chennai, India
  • 2 Samrud Foundation for Health and Research, Bengaluru 560 001, India
  • 3 St. Martha’s Hospital, 5, Nrupatunga Road, Bengaluru 560 001, India
  • 4 Kamal Nayan Bajaj Institute for Research in Vision and Ophthalmology Vision Research Foundation, Chennai, India
  • 5 Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India
Open access

Emergence of extended-spectrum β-lactamase (ESBL) and fluoroquinolone resistance among ocular Enterobacteriaceae is increasing in higher frequency. Therefore, studies are being carried out to understand their multidrug resistance pattern. A total of 101 Enterobacteriaceae isolates recovered from various ocular diseases in a tertiary eye care center at Chennai, India during the period of January 2011 to June 2014 were studied. Forty one randomly chosen isolates were subjected to antibiotic susceptibility by minimum inhibitory concentration (MIC) and genotypic analysis. Of them, 16 were ESBL producers, one was carbapenemase producer and four were resistant to ertapenem which could be due to porin loss associated with AmpC production, and 17 were resistant to fluoroquinolones. Sixteen isolates harbored ESBL genes in which 14 had more than one gene and none of them were positive for blaNDM-1 gene. QNR genes were detected in 18 isolates.

ESBL producers were predominantly isolated from conjunctiva. A high degree of ESBL production and fluoroquinolone resistance is seen among the genus Klebsiella sp. Hence, monitoring the rate of ESBL prevalence plays a vital role in the administration of appropriate intravitreal antibiotics to save the vision and also to reduce the development of drug resistance in ocular pathogens.

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  • 1.

    Bradford PA : Extended-spectrum ß-lactamases in the 21st century: characterization, epidemiology and detection of this important resistance threat. Clin Microbiol Rev 14, 933951 (2001)

    • Search Google Scholar
    • Export Citation
  • 2.

    Coque TM , Baquero F, Cantòn R: Increasing prevalence of ESBL-producing Enterobacteriaceae in Europe. Euro Surveill 13(40–51), 573583 (2008)

    • Search Google Scholar
    • Export Citation
  • 3.

    Lascols C , Robert J, Cattoir V, Bébéar C, Cavallo JD, Podglajen I, Ploy MC, Bonnet R, Soussy CJ, Cambau E: Type II topoisomerase mutations in clinical isolates of Enterobacter cloacae and other enterobacterial species harbouring the qnrA gene. Int J Antimicrob Agents 29(4), 402409 (2007)

    • Search Google Scholar
    • Export Citation
  • 4.

    Nordmann P , Poirel L: Emergence of plasmid-mediated resistance to quinolones in Enterobacteriaceae. J Antimicrob Chemother 56, 463469 (2005)

    • Search Google Scholar
    • Export Citation
  • 5.

    Ozgumus OB , Tosun I, Aydin F, Kilic AO: Horizontal dissemination of TEM- and SHV-type ß-lactamase genescarrying resistance plasmids amongst clinical isolates of Enterobacteriaceae. Brazil J Microbiol 39, 636643 (2008)

    • Search Google Scholar
    • Export Citation
  • 6.

    Nordmann P , Poirel L, Toleman MA, Walsh TR: Does broad-spectrum ß-lactam resistance due to NDM-1 herald the end of the antibiotic era for treatment of infections caused by Gram-negative bacteria? J Antimicrob Chemother 66, 689692 (2011)

    • Search Google Scholar
    • Export Citation
  • 7.

    Duggirala A , Joseph J, Sharma S, Nutheti R, Garg P, Das T: Activity of newer fluoroquinolones against gram-positive and gram-negative bacteria isolated from ocular infections: an in vitro comparison. Indian J Ophthalmol Jan–Feb 55(1), 1519 (2007)

    • Search Google Scholar
    • Export Citation
  • 8.

    Sowmiya M , Malathi J, Madhavan HN: Screening of ocular Enterobacteriaceae isolates for presence of chromosomal blaNDM-1 and ESBL genes: a 2-year study at a tertiary eye care center. Invest Ophthalmol Vis Sci 53, 52515257 (2012)

    • Search Google Scholar
    • Export Citation
  • 9.

    Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing; 24th Informational Supplement Clinical and Laboratory Standards Institute, Wayne, PA. CLSI M100-S24 (2014)

  • 10.

    Pitout JDD , Hossain A, Hanson ND: Phenotypic and Molecular Detection of CTX-M-Beta-Lactamases produced by Escherichia coli and Klebsiella spp. J. Clin. Microbiol 42, 57155721 (2004)

    • Search Google Scholar
    • Export Citation
  • 11.

    Eckert C , Gautier V, Arlet G: DNA sequence analysis of the genetic environment of various blaCTX-M genes. J Antimicrob Chemother 57, 1423 (2006)

    • Search Google Scholar
    • Export Citation
  • 12.

    M’Zali FH , Gascoyne-Binzi DM, Heritage J, Hawkey PM: Detection of mutations conferring extended-spectrum activity on SHV ß-lactamases using polymerase chain reaction single strand conformational polymorphism (PCRSSCP). J. Antimicrob. Chemother 37, 797802 (1996)

    • Search Google Scholar
    • Export Citation
  • 13.

    Weigel LM , Steward CD, Tenover FC: gyrA mutations associated with flouroquinolone resistance in 8 species of Enterobacteriaceae. Antimicrob Agents Chemother 42, 26612667 (1998)

    • Search Google Scholar
    • Export Citation
  • 14.

    Cattoir V , Poirel L, Rotimi V, Soussy CJ, Nordmann P: Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates. J Antimicrob Chemother 60(2), 394397 (2007)

    • Search Google Scholar
    • Export Citation
  • 15.

    Wiener J , Quinn JP, Bradford PA, Goering RV, Nathan C, Bush K, Weinstein RA: Multiple antibiotic-resistant Klebsiella and Escherichia coli in nursing homes. JAMA 281, 517523 (1999)

    • Search Google Scholar
    • Export Citation
  • 16.

    Heisig P : Genetic evidence for a role of parC mutations in development of high-level fluoroquinolone resistance in Escherichia coli. Antimicrob Agents Chemother 40, 879885 (1996)

    • Search Google Scholar
    • Export Citation
  • 17.

    Pazhani GP , Chakraborty S, Fujihara K, Yamasaki S, Ghosh A, Nair GB, Ramamurthy T: QRDR mutations, efflux system and antimicrobial resistance genes in enterotoxigenic Escherichia coli isolated from an outbreak of diarrhoea in Ahmedabad, India. Indian J Med Res 134, 214223 (2011)

    • Search Google Scholar
    • Export Citation
  • 18.

    Oethinger M , Kern WV, Jellen-Ritter AS, McMurry LM, Levy SB: Ineffectiveness of topoisomerase mutations in mediating clinically significant fluoroquinolone resistance in Escherichia coli in the absence of the AcrAB efflux pump. Antimicrob Agents Chemother Jan 44(1), 1013 (2000)

    • Search Google Scholar
    • Export Citation
  • 19.

    Deguchi T , Fukuoka A, Yasuda M, Nakano M, Ozeki S, Kanematsu E, Nishino Y, Ishihara S, Ban Y, Kawada Y: Alteration in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV in quinolone-resistant clinical isolates of Klebsiella pneumoniae. Antimicrob Agents Chemother 41, 699701 (1997)

    • Search Google Scholar
    • Export Citation
  • 20.

    Briales A , Rodríguez-Martínez JM, Velasco C, Díazdelba P, Domínguez-Herrera J, Pachón J, Pascual A: In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC. Antimicrob Agents Chemother 55(3), 12661269 (2011)

    • Search Google Scholar
    • Export Citation

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