View More View Less
  • 1 University of Nangui-Abrogoua, 02 BP 801, Abidjan 02, Côte d’Ivoire
  • | 2 Institut Pasteur of Côte d’Ivoire (IPCI), 01 BP 490, Abidjan 01, Côte d’Ivoire
  • | 3 University of Félix Houphouët Boigny, 01 BP V4, Abidjan 01, Côte d’Ivoire
  • | 4 University of Moncton, Moncton, NB E1A 3E9, Canada
Open access

Pseudomonas aeruginosa owns a variability of virulence factors. These factors can increase bacterial pathogenicity and infection severity. Despite the importance of knowledge about them, these factors are not more characterized at level of strains derived from local food products. This study aimed to characterize the virulence potential of P. aeruginosa isolated from various animal products. Several structural and virulence genes of P. aeruginosa including lasB, exoS, algD, plcH, pilB, exoU, and nan1 were detected by polymerase chain reaction (PCR) on 204 strains of P. aeruginosa. They were isolated from bovine meat (122), fresh fish (49), and smoked fish (33). The 16S rRNA gene was detected on 91.1% of the presumptive strains as Pseudomonas. The rpoB gene showed that 99.5% of the strains were P. aeruginosa. The lasB gene (89.2%) was the most frequently detected (p < 0.05). In decreasing importance order, exoS (86.8%), algD (72.1%), plcH (72.1%), pilB (40.2%), and exoU (2.5%) were detected. The lasB gene was detected in all strains of P. aeruginosa serogroups O11 and O16. The prevalence of algD, exoS, and exoU genes in these strains varied from 51.2% to 87.4%. The simultaneous determination of serogroups and virulence factors is of interest for the efficacy of surveillance of infections associated with P. aeruginosa.

  • 1.

    Edit K , Sándor S, Gyula D, Júlia R, Balázs K, Balázs K: Pathogenic and phylogenetic features of 2 multiresistant Pseudomonas aeruginosa strains originated from remediated sites. Int J Occup Med Environ Health 29, 503516 (2016)

    • Search Google Scholar
    • Export Citation
  • 2.

    Fadhil L , Al-Marzoqi AH, Zahraa MA, Shalan AA: Molecular and phenotypic study of virulence genes in a pathogenic strain of Pseudomonas aeruginosa isolated from various clinical origins by PCR: profiles of genes and toxins. Res J Pharm, Biol Chem Sci 7, 590598 (2016)

    • Search Google Scholar
    • Export Citation
  • 3.

    Streeter K , Katouli M: Pseudomonas aeruginosa: a review of their pathogenesis and prevalence in clinical settings and the environment. Infect Epidemiol Med 2, 2532 (2016)

    • Search Google Scholar
    • Export Citation
  • 4.

    Mitov I , Tanya S, Boyka M: Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of Pseudomonas aeruginosa. Braz J Microbiol 41, 588595 (2010)

    • Search Google Scholar
    • Export Citation
  • 5.

    Khattab MA , Nour MS, ElSheshtawy NM: Genetic identification of Pseudomonas aeruginosa virulence genes among different isolates. J Microb Biochem Technol 7, 274277 (2015)

    • Search Google Scholar
    • Export Citation
  • 6.

    Amazian K , Rossello J, Castella A, Sekkat S, Terzaki S, Dhidah L: Prevalence of nosocomial infections in 27 hospitals in the Mediterranean region. East Mediterr Health J 16, 10701078 (2010)

    • Search Google Scholar
    • Export Citation
  • 7.

    AL-Kadhmi NA , AL-Thwaini AN, AL-Turk WA, AL-Taif KI: Studies on the multidrug resistance to Pseudomonas aeruginosa isolated from infected wounds. Int J Curr Microbiol App Sci 5, 963970 (2016)

    • Search Google Scholar
    • Export Citation
  • 8.

    Kakupa DK , Muenze PK, Byl B, Wilm MD: Study of the prevalence of nosocomial infections and associated factors in the two university hospitals of Lubumbashi, Democratic Republic of Congo. Pan Afr Med J 24, 275280 (2016)

    • Search Google Scholar
    • Export Citation
  • 9.

    Bricha S , Ounine K, Oulkheir S, Haloui N, Attarassi B: Virulence factors and epidemiology related to Pseudomonas aeruginosa. Tunis J Infect Dis 2, 714 (2009)

    • Search Google Scholar
    • Export Citation
  • 10.

    Minchella A , Molinari L, Alonso S, Bouziges N, Sotto A, Lavigne JP: Evolution of antimicrobial resistance against Pseudomonas aeruginosa in a French university hospital between 2002 and 2006. Pathol Biol (Paris) 58, 16 (2010)

    • Search Google Scholar
    • Export Citation
  • 11.

    Vitkauskiene A , Skrodeniene E, Dambrauskiene A, Macas A, Sakalauskas R: Pseudomonas aeruginosa bacteremia: resistance to antibiotics, risk factors, and patient mortality. Medicina (Kaunas) 46, 490495 (2010)

    • Search Google Scholar
    • Export Citation
  • 12.

    Bhattarai RR , Nawaz MA: Isolation and identification of Yersinia and Pseudomonas sp. from Australian milk and salad using 16s rDNA. Peer J Pre Prints 7, 17 (2015)

    • Search Google Scholar
    • Export Citation
  • 13.

    Virupakshaiah DBM , Hemalata VB: Molecular identification of Pseudomonas aeruginosa from food borne isolates. Int J Curr Microbiol App Sci 5, 10261032 (2016)

    • Search Google Scholar
    • Export Citation
  • 14.

    Nikbin V , Aslani M, Sharafi Z, Hashemipour M, Shahcheraghi F, Ebrahimipour G: Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins. Iranian J Microbiol 4, 118123 (2012)

    • Search Google Scholar
    • Export Citation
  • 15.

    Gupta SK , Berk RS, Masinick S, Hazlett LD: Pili and lipopolysaccharide of Pseudomonas aeruginosa bind to the glycolipid asialo GM1. Infect Immun 62, 45724579 (1994)

    • Search Google Scholar
    • Export Citation
  • 16.

    Asikyan ML , Kus JV, Burrows LL: Novel proteins that modulate type IV pilus retraction dynamics in Pseudomonas aeruginosa. J Bacteriol 190, 70227034 (2008)

    • Search Google Scholar
    • Export Citation
  • 17.

    Holloway BW , Römling U, Tümmler B: Genomic mapping of Pseudomonasaeruginosa PAO. Microbiology 140, 29072929 (1994)

  • 18.

    Ghanbarzadeh ZC , Khorshidi A, Firoozeh F, Aznaveh AM, Akbari H: Biofilm formation and virulence factors among Pseudomonas aeruginosa isolated from burn patients. Jundishapur J Microbiol 8, e22345 (2015)

    • Search Google Scholar
    • Export Citation
  • 19.

    Sawa T , Shimizu M, Moriyama K, Wiener-Kronish JP: Association between Pseudomonas aeruginosa type III secretion, antibiotic resistance, and clinical outcome: a review. Critical Care 18, 111 (2014)

    • Search Google Scholar
    • Export Citation
  • 20.

    Stirling FR , Cuzick A, Kelly SM, Oxley D, Evans TJ: Eukaryotic localization, activation and ubiquitinylation of a bacterial type III secreted toxin. Cell Microbiol 8, 12941309 (2006)

    • Search Google Scholar
    • Export Citation
  • 21.

    Saliba AM , Nascimento DO, Silva MC, Assis MC, Gayer CR, Raymond B, Coelho MG, Marques EA, Touqui L, Albano RM, Lopes UG, Paiva DD, Bozza PT, Plotkowskiet MC: Eicosanoid-mediated proinflammatory activity of Pseudomonas aeruginosa ExoU. Cellular Microbiol 7, 18111822 (2005)

    • Search Google Scholar
    • Export Citation
  • 22.

    Lee VT , Smith RS, Tummler BLS: Activities of Pseudomonas aeruginosa effectors secreted by the type III secretion system in vitro and during infection. Infect Immun 73, 16951705 (2005)

    • Search Google Scholar
    • Export Citation
  • 23.

    Zimmermann A , Lüth J, Pauli U: Quantitative and qualitative evaluation of nine different extraction methods for nucleic acids on soya bean food samples. Z Lebensm Unters Forsch 207, 8190 (1998)

    • Search Google Scholar
    • Export Citation
  • 24.

    Franzetti L , Scarpellini M: Characterization of Pseudomonas spp. isolated from foods. Annu Microbiol 57, 3947 (2007)

  • 25.

    Ait-Tayeb L , Ageron E, Grimont F, Grimont, PAD: Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates. Res Microbiol 156, 763773 (2005)

    • Search Google Scholar
    • Export Citation
  • 26.

    De Jonghe V , Coorevits A, Van Hoorde K, Messens W, Landschoot A, De Vos P, Heyndrickx M: Influence of storage conditions on the growth of Pseudomonas species in refrigerated raw milk. Appl Environ Microbiol 77(2), 460470 (2011)

    • Search Google Scholar
    • Export Citation
  • 27.

    Lanotte P , Watt S, Mereghetti L, Dartiguelongue N, Rastegar-Lari A, Goudeau A, Quentin R: Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins. J Med Microbiol 53, 7381 (2004)

    • Search Google Scholar
    • Export Citation
  • 28.

    Strateva T (2008): Microbiological and molecular-genetic investigations on resistance mechanisms and virulence factors in clinical strains of Pseudomonas aeruginosa. Sofia, Bulgaria, PhD Dissertation, Medical University of Sofia, Bulgaria, p. 210

    • Search Google Scholar
    • Export Citation
  • 29.

    Mehri I , Turki Y, Daly I, Rjab AB, Hassen A, Maher G: Molecular identification and assessment of genetic diversity of fluorescent Pseudomonads based on different polymerase chain reaction (PCR) methods. Afr J Microbiol Res 7, 21032113 (2013)

    • Search Google Scholar
    • Export Citation
  • 30.

    Mulet M , Lalucat J, Garcia-Valdés E: DNA sequence-based analysis of the Pseudomonas species. Environ Microbiol 12, 15131530 (2010)

  • 31.

    Whitney J C , Whitfield GB, Marmont LS, Yip P, Neculai AM, Lobsanov Y D, Robinson H, Ohman DE, Howell PL: Dimeric c-di-GMP is required for posttranslational regulation of alginate production in Pseudomonas aeruginosa. J Biol Chem 290, 1245112462 (2015)

    • Search Google Scholar
    • Export Citation
  • 32.

    Høiby N , Bjarnsholt T, Givskov M, Molin S, Ciofu O: Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents 35, 322332 (2010)

    • Search Google Scholar
    • Export Citation
  • 33.

    Barker AP , Vasil AI, Filloux A, Ball G, Wilderman PJ, Vasil ML: A novel extracellular phospholipase C of Pseudomonas aeruginosa is required for phospholipid chemotaxis. Mol Microbiol 53, 10891098 (2004)

    • Search Google Scholar
    • Export Citation
  • 34.

    Jackson AA , Gross MJ, Daniels EF, Hampton TH, Hammons JH, Vallet-Gely I: Anr and its activation by PlcH activity in Pseudomonas aeruginosa host colonization and virulence. J Bacteriol 195, 30933104 (2013)

    • Search Google Scholar
    • Export Citation
  • 35.

    Le-Berre R , Nguyen S, Nowak E, Kipnis E, Pierre M, Quenee L, Ader F, Lancel S, Courcol R, Guery BP, Faure K: Relative contribution of three main virulence factors in Pseudomonas aeruginosa pneumonia. Crit Care Med 39, 21132120 (2011)

    • Search Google Scholar
    • Export Citation
  • 36.

    Lu Q , Philippe E, Charles-Edouard L, Michel W, Michael T, Emmanuelle M, Jorge G, Pierre-François L, Holger K, Bruno F, Verena G, Erkan M, Antonio P, Hedvika L, Chastre J, Rouby JJ: Pseudomonas aeruginosa serotypes in nosocomial pneumonia : prevalence and clinical outcomes. Critic Care 18, R17 (2014)

    • Search Google Scholar
    • Export Citation
  • 37.

    Lu Q , Rouby JJ, Laterre PF, Eggimann P, Dugard A, Giamarellos-Bourboulis EJ, Mercier E, Garbino J, Luyt CE, Chastre J, Georgescu-Kyburz V, Rudolf MP, Gafner V, Lazar H, Koch H, Perez A, Kramer SD, Tamm M: Pharmacokinetics and safety of panobacumab: specific adjunctive immunotherapy in critical patients with nosocomial Pseudomonas aeruginosa O11 pneumonia. J Antimicrob Chemother 66, 11101116 (2011)

    • Search Google Scholar
    • Export Citation
The author instruction is available in PDF.
Please, download the file from HERE.

Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Vice Editor(s)-in-Chief: Fuchs, Anja

Editorial Board

Chair of the Editorial Board:
Jeffrey S. Buguliskis (Thomas Jefferson University, USA)

  • Jörn Albring (University of Münster, Germany)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Megdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Jeff Buguliskis (Thomas Jefferson University, USA)
  • Edit Buzas (Semmelweis University, Hungary)
  • Charles Collyer (University of Sydney, Australia)
  • Renato Damatta (UENF, Brazil)
  • Ivelina Damjanova (Semmelweis University, Hungary)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Anna Erdei (Eötvös Loránd University, Hungary)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Beniam Ghebremedhin (Witten/Herdecke University, Germany)
  • Nancy Guillen (Institute Pasteur, France)
  • Georgina L. Hold (University of Aberdeen, United Kingdom)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Zsuzsanna Izsvak (MDC-Berlin, Germany)
  • Achim Kaasch (University of Cologne, Germany)
  • Tamás Laskay (University of Lübeck, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Shreemanta Parida (Vaccine Grand Challenge Program, India)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Mary Jo Wick (University of Gothenburg, Sweden)
  • Susanne A. Wolf (MDC-Berlin, Germany)


Dr. Dunay, Ildiko Rita
Magdeburg, Germany

Indexing and Abstracting Services:

  • PubMed Central
  • Scopus
  • ESCI
  • CABI


CrossRef Documents 23
WoS Cites 708
Wos H-index 27
Days from submission to acceptance 219
Days from acceptance to publication 176
Acceptance Rate 70%



European Journal of Microbiology and Immunology
Publication Model Gold Open Access
Submission Fee none
Article Processing Charge 928 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

European Journal of Microbiology and Immunology
Language English
Size A4
Year of
2021 Volume 11
per Year
per Year
Founder Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Chief Executive Officer, Akadémiai Kiadó
ISSN 2062-509X (Print)
ISSN 2062-8633 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
May 2021 0 12 28
Jun 2021 0 26 33
Jul 2021 0 20 18
Aug 2021 0 18 28
Sep 2021 0 16 31
Oct 2021 0 41 37
Nov 2021 0 0 0