View More View Less
  • 1 Charité — University Medicine Berlin, Berlin, Germany
Open access

The progressive rise in multidrug-resistant (MDR) bacterial strains poses serious problems in the treatment of infectious diseases. While the number of newly developed antimicrobial compounds has greatly fallen, the resistance of pathogens against commonly prescribed drugs is further increasing. This rise in resistance illustrates the need for developing novel therapeutic and preventive antimicrobial options. The medicinal herb Nigella sativa and its derivatives constitute promising candidates. In a comprehensive literature survey (using the PubMed data base), we searched for publications on the antimicrobial effects of N. sativa particularly directed against MDR bacterial strains. In vitro studies published between 2000 and 2015 revealed that N. sativa exerted potent antibacterial effects against both Gram-positive and Gram-negative species including resistant strains. For instance, N. sativa inhibited the growth of bacteria causing significant gastrointestinal morbidity such as Salmonella, Helicobacter pylori, and Escherichia coli. However, Listeria monocytogenes and Pseudomonas aeruginosa displayed resistance against black cumin seed extracts. In conclusion, our literature survey revealed potent antimicrobial properties of N. sativa against MDR strains in vitro that should be further investigated in order to develop novel therapeutic perspectives for combating infectious diseases particularly caused by MDR strains.

  • 1.

    Fernández J , Bert F, Nicolas-Chanoine MH: The challenges of multi-drug-resistance in hepatology. J Hepatol 65, 10431054 (2016)

  • 2.

    Avner BS , Fialho AM, Chakrabarty AM: Overcoming drug resistance in multi-drug resistant cancers and microorganisms: a conceptual framework. Bioengineered 3, 262270 (2012)

    • Search Google Scholar
    • Export Citation
  • 3.

    Xia J , Gao J, Tang W: Nosocomial infection and its molecular mechanisms of antibiotic resistance. Biosci Trends 10, 1421 (2016)

  • 4.

    Bush K , Bradford PA: ß-Lactams and ß-lactamase inhibitors: an overview. Cold Spring Harb Perspect Med 6(8), pii: a025247 (2016)

  • 5.

    Joo HS , Otto M: Mechanisms of resistance to antimicrobial peptides in staphylococci. Biochim Biophys Acta 1848, 30553061 (2015)

  • 6.

    Reyes K , Bardossy AC, Zervos M: Vancomycin-resistant enterococci: epidemiology, infection prevention, and control. Infect Dis Clin North Am 30, 953965 (2016)

    • Search Google Scholar
    • Export Citation
  • 7.

    Calixto JB : Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Braz J Med Biol Res 33, 179189 (2000)

    • Search Google Scholar
    • Export Citation
  • 8.

    Burits M , Bucar F: Antioxidant activity of Nigella sativa essential oil. Phytother Res 14, 323328 (2000)

  • 9.

    El-Tahir KE-DH , Bakeet DM: The black seed Nigella sativa Linnae21us-A mine for multi cures: a plea for urgent clinical evaluation of its volatile oil. J Taibah Univ Med Sci 1, 119 (2006)

    • Search Google Scholar
    • Export Citation
  • 10.

    Nickavar B , Mojab F, Javidnia K, Amoli MA: Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. Z Naturforsch C 58(9–10), 629631 (2003)

    • Search Google Scholar
    • Export Citation
  • 11.

    Shaaban HA , Sadek Z, Edris AE, Saad-Hussein A: Analysis and antibacterial activity of Nigella sativa essential oil formulated in microemulsion system. J Oleo Sci 64, 223232 (2015)

    • Search Google Scholar
    • Export Citation
  • 12.

    Ali BH , Blunden G: Pharmacological and toxicological properties of Nigella sativa. Phytother Res 17, 299305 (2003)

  • 13.

    Salim EI , Fukushima S: Chemopreventive potential of volatile oil from black cumin (Nigella sativa L.) seeds against rat colon carcinogenesis. Nutr Cancer 45, 195202 (2003)

    • Search Google Scholar
    • Export Citation
  • 14.

    Singh AP , Moniaux N, Chauhan SC, Meza JL, Batra SK: Inhibition of MUC4 expression suppresses pancreatic tumor cell growth and metastasis. Cancer Res 64, 622630 (2004)

    • Search Google Scholar
    • Export Citation
  • 15.

    Banerjee S , Kaseb AO, Wang Z, Kong DM, Mussop P, Subhash S, Fazlul HM, Ramzi M: Antitumor activity of gemcitabine and oxaliplatin is augmented by thymoquinone in pancreatic cancer. Cancer Res 69, 55755583 (2009)

    • Search Google Scholar
    • Export Citation
  • 16.

    Dehkordi FR , Kamkhah AF: Antihypertensiveeffect of Nigella sativa seed extract in patients with mild hypertension. Fundam Clin Pharmacol 22, 447452 (2008)

    • Search Google Scholar
    • Export Citation
  • 17.

    Boskabady M , Mohsenpoor N, Takaloo L: Antiasthmatic effect of Nigella sativa in airways of asthmatic patients. Phytomedicine 17, 707713 (2010)

    • Search Google Scholar
    • Export Citation
  • 18.

    El-Abhar H , Abdallah D, Saleh S: Gastroprotective activity of Nigella sativa oil and its constituent, thymoquinone, against gastric mucosal injury induced by ischaemia/reperfusion in rats. J Ethnopharmacol 84, 251258 (2003)

    • Search Google Scholar
    • Export Citation
  • 19.

    Gholamnezhad Z , Keyhanmanesh R, Boskabady MH: Anti-inflammatory, antioxidant, and immunomodulatory aspects of Nigella sativa for its preventive and bronchodilatatory effects on obstructive respiratory diseases: a review of basic and clinical evidence. J Funct Food 17, 910927 (2015)

    • Search Google Scholar
    • Export Citation
  • 20.

    Sarwar A , Latif Z: GC-MS characterisation and antibacterial activity evaluation of Nigella sativa oil against diverse strains of Salmonella. Nat Prod Res 29, 447451 (2015)

    • Search Google Scholar
    • Export Citation
  • 21.

    O’Mahony R , Al-Khtheeri H, Weerasekera D, Fernando N, Vaira D, Holton J, Bassett C: Bactericidal and anti-adhesive properties of culinary and medicinal plants against Helicobacter pylori. World J Gastroenterol 11, 74997507 (2005)

    • Search Google Scholar
    • Export Citation
  • 22.

    Bacha K , Tariku Y, Gebreyesus F, Zerihun S, Mohammed A, Weiland-Bräuer N, Schmitz RA, Mulat M: Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: implication for development of potent antimicrobial agents. BMC Microbiol 16, 139 (2016)

    • Search Google Scholar
    • Export Citation
  • 23.

    Emeka LB , Emeka PM, Khan TM: Antimicrobial activity of Nigella sativa L. seed oil against multi-drug resistant Staphylococcus aureus isolated from diabetic wounds. Pak J Pharm Sci 28, 19851990 (2015)

    • Search Google Scholar
    • Export Citation
  • 24.

    Liaqat F , Sheikh AA, Nazir J, Hussain T, Rabbani M, Shaheen AY, Muhammed J: Report-isolation identification and control of vancomycin resistant Staphylococcus aureus. Pak J Pharm Sci 28, 9971004 (2015)

    • Search Google Scholar
    • Export Citation
  • 25.

    Hannan A , Saleem S, Chaudhary S, Barkaat M, Arshad MU: Anti-bacterial activity of Nigella sativa against clinical isolates of methicillin resistant Staphylococcus aureus. J Ayub Med Coll Abbottabad 20, 7274 (2008)

    • Search Google Scholar
    • Export Citation
  • 26.

    Boka J , Mahdavi A, Samie A, Jahanian R: Effect of different levels of black cumin (Nigella sativa L.) on performance, intestinal Escherichia coli colonization and jejunal morphology in laying hens. J Anim Physiol Anim Nutr 98, 373383 (2014)

    • Search Google Scholar
    • Export Citation
  • 27.

    Kapan M , Tekin R, Onder A, Firat U, Evliyaoglu O, Taskesen F, Arikanoglu Z: Thymoquinone ameliorates bacterial translocation and inflammatory response in rats with intestinal obstruction. Int J Surg 10, 484488 (2012)

    • Search Google Scholar
    • Export Citation
  • 28.

    Salem EM , Yar T, Bamosa A, Al-Quorain A, Yasawy MI, Alsulaiman RM, Randhawa MA: Comparative study of Nigella Sativa and triple therapy in eradication of Helicobacter Pylori in patients with non-ulcer dyspepsia. Saudi J Gastroenterol 16, 207214 (2010)

    • Search Google Scholar
    • Export Citation
The author instruction is available in PDF.
Please, download the file from HERE.

Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Vice Editor(s)-in-Chief: Fuchs, Anja

Editorial Board

Chair of the Editorial Board:
Jeffrey S. Buguliskis (Thomas Jefferson University, USA)

  • Jörn Albring (University of Münster, Germany)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Megdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Jeff Buguliskis (Thomas Jefferson University, USA)
  • Edit Buzas (Semmelweis University, Hungary)
  • Charles Collyer (University of Sydney, Australia)
  • Renato Damatta (UENF, Brazil)
  • Ivelina Damjanova (Semmelweis University, Hungary)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Anna Erdei (Eötvös Loránd University, Hungary)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Beniam Ghebremedhin (Witten/Herdecke University, Germany)
  • Nancy Guillen (Institute Pasteur, France)
  • Georgina L. Hold (University of Aberdeen, United Kingdom)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Zsuzsanna Izsvak (MDC-Berlin, Germany)
  • Achim Kaasch (University of Cologne, Germany)
  • Tamás Laskay (University of Lübeck, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Shreemanta Parida (Vaccine Grand Challenge Program, India)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Mary Jo Wick (University of Gothenburg, Sweden)
  • Susanne A. Wolf (MDC-Berlin, Germany)


Dr. Dunay, Ildiko Rita
Magdeburg, Germany

Indexing and Abstracting Services:

  • PubMed Central
  • Scopus
  • ESCI
  • CABI


CrossRef Documents 23
WoS Cites 708
Wos H-index 27
Days from submission to acceptance 219
Days from acceptance to publication 176
Acceptance Rate 70%



European Journal of Microbiology and Immunology
Publication Model Gold Open Access
Submission Fee none
Article Processing Charge 928 EUR/article
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Gold Open Access
Purchase per Title  

European Journal of Microbiology and Immunology
Language English
Size A4
Year of
per Year
per Year
Founder Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Chief Executive Officer, Akadémiai Kiadó
ISSN 2062-509X (Print)
ISSN 2062-8633 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2021 0 32 29
Sep 2021 0 36 54
Oct 2021 0 66 60
Nov 2021 0 50 63
Dec 2021 0 33 33
Jan 2022 0 35 45
Feb 2022 0 0 0