Bacterial antimicrobial resistance mediated by the production of extended-spectrum β-lactamases (ESBLs) is considered a major threat for treatment of Salmonella and Shigella infections. This study aimed to investigate antibiotic resistance patterns of Salmonella and Shigella spp. and presence of CTX-M from three teaching hospitals in Iran. In the present study, 58 clinical Shigella and 91 Salmonella isolates were recovered between 2009 and 2013 from 3 teaching hospitals in Iran. After culture and antimicrobial susceptibility testing, ESBL-positive isolates were subjected to further investigations. These included polymerase chain reaction (PCR) amplification and DNA sequencing of blaCTX-M-15 encoding plasmid. In both genera, high sensitivity to gentamicin and amikacin, but high resistance to ampicillin, tetracycline, and trimethoprim—sulfamethoxazole, was found. Molecular investigation showed that 31.8% isolates of Salmonella spp. and 34.48% isolates of Shigella spp. were CTX-M positive and all of them were also positive for ISEcpI. Protein translation, comparing with reference sequences, showed that all CTX-M isolates belong to CTX-M-15. The present study suggests that the resistance of ESBLs-producing Salmonella and Shigella spp. in Iran hospitals is very serious. Therefore, strategies to minimize the spread of ESBL-producing isolates should be implemented.
Bialvaei AZ , Kafil HS, Asgharzadeh M, Yousef Memar M, Yousefi M: Current methods for the identification of carbapenemases. J Chemother 28, 1–19 (2016)
Weill FX , Perrier-Gros-Claude JD, Demartin M, Coignard S, Grimont PA: Characterization of extended-spectrum beta-lactamase (CTX-M-15)-producing strains of Salmonella enterica isolated in France and Senegal. FEMS Microbiol Lett 238, 353–358 (2004)
Kim S , Kim J, Kang Y, Park Y, Lee B: Occurrence of extended-spectrum ß-lactamases in members of the genus Shigella in the Republic of Korea. J. Clin Microbiol 42, 5264–5269 (2004)
Moubareck C , Doucet-Populaire F, Hamze M, Daoud Z, Weill F-X First extended-spectrum-ß-lactamase (CTXM-15)-producing Salmonella enterica serotype Typhimurium isolate identified in Lebanon. Antimicrob Agents Chemother 49, 864–865 (2005)
Nguyen NT , Ha V, Tran NV, Stabler R, Pham DT, et al.: The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. circulating in Southern Vietnam. PLoS Negl Trop Dis 4, e702 (2010)
Tzouvelekis LS , Tzelepi E, Tassios PT, Legakis NJ: CTX-Mtype beta-lactamases: an emerging group of extended-spectrum enzymes. Int J Antimicrob Agents 14, 137–142 (2000)
Garza-Gonzalez E , Mendoza Ibarra SI, Llaca-Diaz JM, Gonzalez GM: Molecular characterization and antimicrobial susceptibility of extended-spectrum beta-lactamaseproducing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico. J Med Microbiol 60, 84–90 (2011)
Karim A , Poirel L, Nagarajan S, Nordmann P: Plasmidmediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol Lett 201, 237–241 (2001)
Bonnet R : Growing group of extended-spectrum ß-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 48, 1–14 (2004)
Heffernan HM , Woodhouse RE, Pope CE, Blackmore TK: Prevalence and types of extended-spectrum beta-lactamases among urinary Escherichia coli and Klebsiella spp. in New Zealand. Int J Antimicrob Agents 34, 544–549 (2009)
Valverde A , Coque TM, Garcia-San Miguel L, Baquero F, Canton R: Complex molecular epidemiology of extendedspectrum beta-lactamases in Klebsiella pneumoniae: a longterm perspective from a single institution in Madrid. J Antimicrob Chemother 61, 64–72 (2008)
Kiratisin P , Apisarnthanarak A, Laesripa C, Saifon P: Molecular characterization and epidemiology of extendedspectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates causing health care-associated infection in Thailand, where the CTX-M family is endemic. Antimicrob Agents Chemother 52, 2818–2824 (2008)
Bialvaei AZ , Samadi Kafil H: Colistin, mechanisms and prevalence of resistance. Curr Med Res Opin 31, 707–721 (2015)
Ranjbar R , Giammanco GM, Aleo A, Plano MRA, Naghoni A, et al.: Characterization of the first extended-spectrum ß-lactamase-producing nontyphoidal Salmonella strains isolated in Tehran, Iran. Foodborne Pathog Dis 7, 91–95 (2010)
Ranjbar R , Ghazi FM, Farshad S, Giammanco GM, Aleo A, et al.: The occurrence of extended-spectrum ß-lactamase producing Shigella spp. in Tehran, Iran. IJM 5, 108 (2013)
Akhi MT , Bialvaei A, Ghotaslou R, Asgharzadeh M, Naghili B, Pirzadeh T, et al.: Faecal carriage of ESBL and plasmidmediated AmpC ß-lactamase genes in Klebsiella spp. and Shigella spp. isolated from inpatient and outpatient carriers in Tabriz, Iran. Int J Fund Appl Sci 8, 16–29 (2016)
Kelly MT , Brenner DJ, Farmer III JJ (1985): Enterobacteriaceae. In: E.H. Lennette, A. Balows, W. Hausler, Jr. and H.J. Shadomy, eds., American Society for Microbiology, Washington, DC
Clinical and Laboratory Standards Institute (2012). M100-S22. Performance standards for antimicrobial susceptibility testing: 22nd informational supplement. CLSI, Wayne, PA
Jarlier V , Nicolas MH, Fournier G, Philippon A: Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis 10, 867–878 (1988)
Thomson KS , Sanders CC: Detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae: comparison of the double-disk and three-dimensional tests. Antimicrob Agents Chemother 36, 1877–1882 (1992)
al Naiemi N , Duim B, Bart AA: CTX-M extended-spectrum beta-lactamase in Pseudomonas aeruginosa and Stenotrophomonas maltophilia. J Med Microbiol 55, 1607–1608 (2006)
Asgharzadeh M , Kafil HS, Khakpour M: Comparison of mycobacterial interspersed repetitive unit-variable number tandem repeat and IS6110-RFLP methods in identifying epidemiological links in patients with tuberculosis in Northwest of Iran. Ann Microbiol 58, 333–339 (2008)
Asgharzadeh M , Kafil HS, Ebrahimzadeh ME, Bohlouli A: Mannose-binding lectin gene and promoter polymorphism and susceptibility to renal dysfunction in systemic lupus erythematosus. J Biol Sci 7, 801–805 (2007)
Mirzaie S , Hassanzadeh M, Ashrafi I: Identification and characterization of Salmonella isolates from captured house sparrows. Turk J Vet Anim Sci 34, 181–186 (2010)
Bialvaei AZ , Kafil HS, Asgharzadeh M, Aghazadeh M, Yousefi M: CTX-M extended-spectrum beta-lactamaseproducing Klebsiella spp., Salmonella spp., Shigella spp. and Escherichia coli isolates in Iranian hospitals. Braz J Microbiol 47, 706–711 (2016)
Yu Y , Ji S, Chen Y, Zhou W, Wei Z, et al.: Resistance of strains producing extended-spectrum ß-lactamases and genotype distribution in China. J Infect 54, 53–57 (2007)
Hamidian M , Tajbakhsh M, Walther-Rasmussen J, Zali MR: Emergence of extended-spectrum beta-lactamases in clinical isolates of Salmonella enterica in Tehran, Iran. Jpn J Infect Dis 62, 368–371 (2009)
Tajbakhsh M , Garcia Migura L, Rahbar M, Svendsen CA, Mohammadzadeh M, et al.: Antimicrobial-resistant Shigella infections from Iran: an overlooked problem? J Antimicrob Chemother 67, 1128–1133 (2012)
Rezaee MA , Abdinia B, Abri R, Kafil HS: Comparison of the antibiotic resistance patterns among Shigella species isolated from pediatric hospital between 1995–1999 and 2009–2013 in North-West of Iran. J Anal Res Clin Med, 2, 118–122 (2014)
George E , Sankar S, Jesudasan M, Sudandiradoss C, Nandagopal B: Molecular characterization of CTX-M-type extended-spectrum beta-lactamase-producing E. coli isolated from humans and the environment. Indian J Med Microbiol 33, 73 (2015)
Mehrgan H , Rahbar M: Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli in a tertiary care hospital in Tehran, Iran. Int J Antimicrob Agents 31, 147–151 (2008)
Ramazanzadeh R , Chitsaz M, Bahmani N: Prevalence and antimicrobial susceptibility of extended-spectrum beta-lactamase-producing bacteria in intensive care units of Sanandaj General Hospitals (Kurdistan, Iran). Chemotherapy 55, 287–292 (2009)
Peerayeh SN , Rostami E, Siadat SD, Derakhshan S: High rate of aminoglycoside resistance in CTX-M-15 producing Klebsiella pneumoniae isolates in Tehran, Iran. Lab Med 45, 231–237 (2014)
Gonullu N , Aktas Z, Kayacan CB, Salcioglu M, Carattoli A, et al.: Dißsemination of CTX-M-15 β-lactamase genes carried on Inc FI and FII plasmids among clinical isolates of Escherichia coli in a University Hospital in Istanbul, Turkey. J Clin Microbiol 46, 1110–1112 (2008)
Matar GM , Kattar MM, Khairallah MT, Abi-Rached R, Mokhbat J: Detection of plasmid-encoded bla-CTX-M-15 and bla-TEM-1 genes in a Lebanese Salmonella isolate that produces extended-spectrum beta-lactamase. Ann Trop Med Parasitol 102, 651–653 (2008)
Rotimi VO , Jamal W, Pal T, Sovenned A, Albert MJ: Emergence of CTX-M-15 type extended-spectrum beta-lactamase-producing Salmonella spp. in Kuwait and the United Arab Emirates. J Med Microbiol 57, 881–886 (2008)