Authors:
Abed Zahedi Bialvaei Iran University of Medical Sciences, Tehran, Iran

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Tala Pourlak Tabriz University of Medical Sciences, Tabriz, Iran

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Mina Aghamali Tabriz University of Medical Sciences, Tabriz, Iran

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Mohammad Asgharzadeh Tabriz University of Medical Sciences, Tabriz, Iran

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Pourya Gholizadeh Tabriz University of Medical Sciences, Tabriz, Iran

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Hossein Samadi Kafil Tabriz University of Medical Sciences, Tabriz, Iran

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Bacterial antimicrobial resistance mediated by the production of extended-spectrum β-lactamases (ESBLs) is considered a major threat for treatment of Salmonella and Shigella infections. This study aimed to investigate antibiotic resistance patterns of Salmonella and Shigella spp. and presence of CTX-M from three teaching hospitals in Iran. In the present study, 58 clinical Shigella and 91 Salmonella isolates were recovered between 2009 and 2013 from 3 teaching hospitals in Iran. After culture and antimicrobial susceptibility testing, ESBL-positive isolates were subjected to further investigations. These included polymerase chain reaction (PCR) amplification and DNA sequencing of blaCTX-M-15 encoding plasmid. In both genera, high sensitivity to gentamicin and amikacin, but high resistance to ampicillin, tetracycline, and trimethoprim—sulfamethoxazole, was found. Molecular investigation showed that 31.8% isolates of Salmonella spp. and 34.48% isolates of Shigella spp. were CTX-M positive and all of them were also positive for ISEcpI. Protein translation, comparing with reference sequences, showed that all CTX-M isolates belong to CTX-M-15. The present study suggests that the resistance of ESBLs-producing Salmonella and Shigella spp. in Iran hospitals is very serious. Therefore, strategies to minimize the spread of ESBL-producing isolates should be implemented.

  • 1.

    Bialvaei AZ , Kafil HS, Asgharzadeh M, Yousef Memar M, Yousefi M: Current methods for the identification of carbapenemases. J Chemother 28, 119 (2016)

    • Search Google Scholar
    • Export Citation
  • 2.

    Weill FX , Perrier-Gros-Claude JD, Demartin M, Coignard S, Grimont PA: Characterization of extended-spectrum beta-lactamase (CTX-M-15)-producing strains of Salmonella enterica isolated in France and Senegal. FEMS Microbiol Lett 238, 353358 (2004)

    • Search Google Scholar
    • Export Citation
  • 3.

    Kim S , Kim J, Kang Y, Park Y, Lee B: Occurrence of extended-spectrum ß-lactamases in members of the genus Shigella in the Republic of Korea. J. Clin Microbiol 42, 52645269 (2004)

    • Search Google Scholar
    • Export Citation
  • 4.

    Moubareck C , Doucet-Populaire F, Hamze M, Daoud Z, Weill F-X First extended-spectrum-ß-lactamase (CTXM-15)-producing Salmonella enterica serotype Typhimurium isolate identified in Lebanon. Antimicrob Agents Chemother 49, 864865 (2005)

    • Search Google Scholar
    • Export Citation
  • 5.

    Nguyen NT , Ha V, Tran NV, Stabler R, Pham DT, et al.: The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. circulating in Southern Vietnam. PLoS Negl Trop Dis 4, e702 (2010)

    • Search Google Scholar
    • Export Citation
  • 6.

    Tzouvelekis LS , Tzelepi E, Tassios PT, Legakis NJ: CTX-Mtype beta-lactamases: an emerging group of extended-spectrum enzymes. Int J Antimicrob Agents 14, 137142 (2000)

    • Search Google Scholar
    • Export Citation
  • 7.

    Garza-Gonzalez E , Mendoza Ibarra SI, Llaca-Diaz JM, Gonzalez GM: Molecular characterization and antimicrobial susceptibility of extended-spectrum beta-lactamaseproducing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico. J Med Microbiol 60, 8490 (2011)

    • Search Google Scholar
    • Export Citation
  • 8.

    Karim A , Poirel L, Nagarajan S, Nordmann P: Plasmidmediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol Lett 201, 237241 (2001)

    • Search Google Scholar
    • Export Citation
  • 9.

    Bonnet R : Growing group of extended-spectrum ß-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 48, 114 (2004)

  • 10.

    Heffernan HM , Woodhouse RE, Pope CE, Blackmore TK: Prevalence and types of extended-spectrum beta-lactamases among urinary Escherichia coli and Klebsiella spp. in New Zealand. Int J Antimicrob Agents 34, 544549 (2009)

    • Search Google Scholar
    • Export Citation
  • 11.

    Valverde A , Coque TM, Garcia-San Miguel L, Baquero F, Canton R: Complex molecular epidemiology of extendedspectrum beta-lactamases in Klebsiella pneumoniae: a longterm perspective from a single institution in Madrid. J Antimicrob Chemother 61, 6472 (2008)

    • Search Google Scholar
    • Export Citation
  • 12.

    Kiratisin P , Apisarnthanarak A, Laesripa C, Saifon P: Molecular characterization and epidemiology of extendedspectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates causing health care-associated infection in Thailand, where the CTX-M family is endemic. Antimicrob Agents Chemother 52, 28182824 (2008)

    • Search Google Scholar
    • Export Citation
  • 13.

    Bialvaei AZ , Samadi Kafil H: Colistin, mechanisms and prevalence of resistance. Curr Med Res Opin 31, 707721 (2015)

  • 14.

    Ranjbar R , Giammanco GM, Aleo A, Plano MRA, Naghoni A, et al.: Characterization of the first extended-spectrum ß-lactamase-producing nontyphoidal Salmonella strains isolated in Tehran, Iran. Foodborne Pathog Dis 7, 9195 (2010)

    • Search Google Scholar
    • Export Citation
  • 15.

    Ranjbar R , Ghazi FM, Farshad S, Giammanco GM, Aleo A, et al.: The occurrence of extended-spectrum ß-lactamase producing Shigella spp. in Tehran, Iran. IJM 5, 108 (2013)

    • Search Google Scholar
    • Export Citation
  • 16.

    Akhi MT , Bialvaei A, Ghotaslou R, Asgharzadeh M, Naghili B, Pirzadeh T, et al.: Faecal carriage of ESBL and plasmidmediated AmpC ß-lactamase genes in Klebsiella spp. and Shigella spp. isolated from inpatient and outpatient carriers in Tabriz, Iran. Int J Fund Appl Sci 8, 1629 (2016)

    • Search Google Scholar
    • Export Citation
  • 17.

    Kelly MT , Brenner DJ, Farmer III JJ (1985): Enterobacteriaceae. In: E.H. Lennette, A. Balows, W. Hausler, Jr. and H.J. Shadomy, eds., American Society for Microbiology, Washington, DC

    • Search Google Scholar
    • Export Citation
  • 18.

    Clinical and Laboratory Standards Institute (2012). M100-S22. Performance standards for antimicrobial susceptibility testing: 22nd informational supplement. CLSI, Wayne, PA

    • Search Google Scholar
    • Export Citation
  • 19.

    Jarlier V , Nicolas MH, Fournier G, Philippon A: Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis 10, 867878 (1988)

    • Search Google Scholar
    • Export Citation
  • 20.

    Thomson KS , Sanders CC: Detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae: comparison of the double-disk and three-dimensional tests. Antimicrob Agents Chemother 36, 18771882 (1992)

    • Search Google Scholar
    • Export Citation
  • 21.

    al Naiemi N , Duim B, Bart AA: CTX-M extended-spectrum beta-lactamase in Pseudomonas aeruginosa and Stenotrophomonas maltophilia. J Med Microbiol 55, 16071608 (2006)

    • Search Google Scholar
    • Export Citation
  • 22.

    Asgharzadeh M , Kafil HS, Khakpour M: Comparison of mycobacterial interspersed repetitive unit-variable number tandem repeat and IS6110-RFLP methods in identifying epidemiological links in patients with tuberculosis in Northwest of Iran. Ann Microbiol 58, 333339 (2008)

    • Search Google Scholar
    • Export Citation
  • 23.

    Asgharzadeh M , Kafil HS, Ebrahimzadeh ME, Bohlouli A: Mannose-binding lectin gene and promoter polymorphism and susceptibility to renal dysfunction in systemic lupus erythematosus. J Biol Sci 7, 801805 (2007)

    • Search Google Scholar
    • Export Citation
  • 24.

    Mirzaie S , Hassanzadeh M, Ashrafi I: Identification and characterization of Salmonella isolates from captured house sparrows. Turk J Vet Anim Sci 34, 181186 (2010)

    • Search Google Scholar
    • Export Citation
  • 25.

    Bialvaei AZ , Kafil HS, Asgharzadeh M, Aghazadeh M, Yousefi M: CTX-M extended-spectrum beta-lactamaseproducing Klebsiella spp., Salmonella spp., Shigella spp. and Escherichia coli isolates in Iranian hospitals. Braz J Microbiol 47, 706711 (2016)

    • Search Google Scholar
    • Export Citation
  • 26.

    Yu Y , Ji S, Chen Y, Zhou W, Wei Z, et al.: Resistance of strains producing extended-spectrum ß-lactamases and genotype distribution in China. J Infect 54, 5357 (2007)

    • Search Google Scholar
    • Export Citation
  • 27.

    Hamidian M , Tajbakhsh M, Walther-Rasmussen J, Zali MR: Emergence of extended-spectrum beta-lactamases in clinical isolates of Salmonella enterica in Tehran, Iran. Jpn J Infect Dis 62, 368371 (2009)

    • Search Google Scholar
    • Export Citation
  • 28.

    Tajbakhsh M , Garcia Migura L, Rahbar M, Svendsen CA, Mohammadzadeh M, et al.: Antimicrobial-resistant Shigella infections from Iran: an overlooked problem? J Antimicrob Chemother 67, 11281133 (2012)

    • Search Google Scholar
    • Export Citation
  • 29.

    Rezaee MA , Abdinia B, Abri R, Kafil HS: Comparison of the antibiotic resistance patterns among Shigella species isolated from pediatric hospital between 1995–1999 and 2009–2013 in North-West of Iran. J Anal Res Clin Med, 2, 118122 (2014)

    • Search Google Scholar
    • Export Citation
  • 30.

    George E , Sankar S, Jesudasan M, Sudandiradoss C, Nandagopal B: Molecular characterization of CTX-M-type extended-spectrum beta-lactamase-producing E. coli isolated from humans and the environment. Indian J Med Microbiol 33, 73 (2015)

    • Search Google Scholar
    • Export Citation
  • 31.

    Mehrgan H , Rahbar M: Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli in a tertiary care hospital in Tehran, Iran. Int J Antimicrob Agents 31, 147151 (2008)

    • Search Google Scholar
    • Export Citation
  • 32.

    Ramazanzadeh R , Chitsaz M, Bahmani N: Prevalence and antimicrobial susceptibility of extended-spectrum beta-lactamase-producing bacteria in intensive care units of Sanandaj General Hospitals (Kurdistan, Iran). Chemotherapy 55, 287292 (2009)

    • Search Google Scholar
    • Export Citation
  • 33.

    Peerayeh SN , Rostami E, Siadat SD, Derakhshan S: High rate of aminoglycoside resistance in CTX-M-15 producing Klebsiella pneumoniae isolates in Tehran, Iran. Lab Med 45, 231237 (2014)

    • Search Google Scholar
    • Export Citation
  • 34.

    Gonullu N , Aktas Z, Kayacan CB, Salcioglu M, Carattoli A, et al.: Dißsemination of CTX-M-15 β-lactamase genes carried on Inc FI and FII plasmids among clinical isolates of Escherichia coli in a University Hospital in Istanbul, Turkey. J Clin Microbiol 46, 11101112 (2008)

    • Search Google Scholar
    • Export Citation
  • 35.

    Matar GM , Kattar MM, Khairallah MT, Abi-Rached R, Mokhbat J: Detection of plasmid-encoded bla-CTX-M-15 and bla-TEM-1 genes in a Lebanese Salmonella isolate that produces extended-spectrum beta-lactamase. Ann Trop Med Parasitol 102, 651653 (2008)

    • Search Google Scholar
    • Export Citation
  • 36.

    Rotimi VO , Jamal W, Pal T, Sovenned A, Albert MJ: Emergence of CTX-M-15 type extended-spectrum beta-lactamase-producing Salmonella spp. in Kuwait and the United Arab Emirates. J Med Microbiol 57, 881886 (2008)

    • Search Google Scholar
    • Export Citation
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Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita, Prof. Dr. Pharm, Dr. rer. nat., University of Magdeburg, Germany

Editor(s)-in-Chief: Heimesaat, Markus M., Prof. Dr. med., Charité - University Medicine Berlin, Germany

Editorial Board

  • Berit Bangoura, Dr. DVM. PhD,  University of Wyoming, USA
  • Stefan Bereswill, Prof. Dr. rer. nat., Charité - University Medicine Berlin, Germany
  • Dunja Bruder, Prof. Dr. rer. nat., University of Magdeburg, Germany
  • Jan Buer, Prof. Dr. med., University of Duisburg, Germany
  • Edit Buzas, Prof. Dr. med., Semmelweis University, Hungary
  • Renato Damatta, Prof. PhD, UENF, Brazil
  • Maria Deli, MD, PhD, DSc, Biological Research Center, HAS, Hungary
  • Olgica Djurković-Djaković, Prof. Phd, University of Belgrade, Serbia
  • Jean-Dennis Docquier, Prof. Dr. med., University of Siena, Italy
  • Zsuzsanna Fabry, Prof. Phd, University of Washington, USA
  • Ralf Ignatius, Prof. Dr. med., Charité - University Medicine Berlin, Germany
  • Achim Kaasch, Prof. Dr. med., Otto von Guericke University Magdeburg, Germany
  • Oliver Liesenfeld, Prof. Dr. med., Inflammatix, USA
  • Matyas Sandor, Prof. PhD, University of Wisconsin, USA
  • Ulrich Steinhoff, Prof. PhD, University of Marburg, Germany
  • Michal Toborek, Prof. PhD, University of Miami, USA
  • Susanne A. Wolf, PhD, MDC-Berlin, Germany

 

Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildiko.dunay@med.ovgu.de

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European Journal of Microbiology and Immunology
Language English
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