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  • 1 Bundeswehr Hospital Hamburg, Hamburg, Germany
  • | 2 University Medicine Rostock, Rostock, Germany
  • | 3 Charité – University Medicine Berlin, Berlin, Germany
  • | 4 Bernhard Nocht Institute for Tropical Medicine Hamburg, Hamburg, Germany
  • | 5 Bundeswehr Medical Academy, Munich, Germany
  • | 6 University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • | 7 Central Institute of the Bundeswehr Medical Service Koblenz, Koblenz, Germany
  • | 8 University of Antananarivo, Antananarivo, Madagascar
Open access

Direct growth on blood and screening agar for methicillin-resistant Staphylococcus aureus (MRSA) at a tropical surveillance site was compared with broth enrichment and subsequent growth on selective MRSA agar after international sample transport.

In Madagascar, 1548 swabs from an MRSA surveillance study were assessed for growth on Columbia blood agar enriched with 5% sheep blood and MRSA screening agar at the surveillance site with subsequent cold storage of the samples and shipment to Germany. In Germany, 1541 shipped samples were analyzed by non-selective broth enrichment with subsequent culture on MRSA selective agar.

A total of 28 MRSA isolates were detected. Of these, 20 strains were isolated from direct culture on blood and MRSA screening agars at the surveillance site, 24 MRSA strains were isolated using the broth enrichment method in Germany, and 16 MRSA strains were identified by both approaches.

In spite of the observed die-off of individual strains due to long-term storage and transport, broth enrichment with subsequent screening on MRSA selective agar after international sample shipment led to comparable sensitivity of MRSA detection like streaking on blood and MRSA agar at the tropical surveillance site.

  • 1.

    Shears P : Antibiotic resistance in the tropics. Epidemiology and surveillance of antimicrobial resistance in the tropics. Trans R Soc Trop Med Hyg 95, 127130 (2001)

    • Search Google Scholar
    • Export Citation
  • 2.

    Tong SY , Steer AC, Jenney AW, Carapetis JR: Communityassociated methicillin-resistant Staphylococcus aureus skin infections in the tropics. Dermatol Clin 29, 2132 (2011)

    • Search Google Scholar
    • Export Citation
  • 3.

    Breurec S , Zriouil SB, Fall C, Boisier P, Brisse S, Djibo S, Etienne J, Fonkoua MC, Perrier-Gros-Claude JD, Pouillot R, Ramarokoto CE, Randrianirina F, Tall A, Thiberge JM, Working Group on Staphylococcus aureus infections, Laurent F, Garin B: Epidemiology of methicillin-resistant Staphylococcus aureus lineages in five major African towns: emergence and spread of atypical clones. Clin Microbiol Infect 17, 160165 (2011)

    • Search Google Scholar
    • Export Citation
  • 4.

    Warnke P , Devide A, Weise M, Frickmann H, Schwarz NG, Schäffler H, Ottl P, Podbielski A: Utilizing moist or dry swabs for the sampling of nasal MRSA carriers? An in vivo and in vitro study. PLoS One 11, e0163073 (2016)

    • Search Google Scholar
    • Export Citation
  • 5.

    Warnke P , Frickmann H, Ottl P, Podbielski A: Nasal screening for MRSA: different swabs – different results! PLoS One 9, e111627 (2014)

  • 6.

    Warnke P , Harnack T, Ottl P, Kundt G, Podbielski A: Nasal screening for Staphylococcus aureus – daily routine with improvement potentials. PLoS One 9, e89667 (2014)

    • Search Google Scholar
    • Export Citation
  • 7.

    Warnke P , Warning L, Podbielski A: Some are more equal – a comparative study on swab uptake and release of bacterial suspensions. PLoS One 9, e102215 (2014)

    • Search Google Scholar
    • Export Citation
  • 8.

    Wertheim H , Verbrugh HA, van Pelt C, de Man P, van Belkum A, Vos MC: Improved detection of methicillin-resistant Staphylococcus aureus using phenyl mannitol broth containing aztreonam and ceftizoxime. J Clin Microbiol 39, 26602662 (2001)

    • Search Google Scholar
    • Export Citation
  • 9.

    Fang H , Hedin G: Use of cefoxitin-based selective broth for improved detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 44, 592594 (2006)

    • Search Google Scholar
    • Export Citation
  • 10.

    French GL : Methods for screening for methicillin-resistant Staphylococcus aureus carriage. Clin Microbiol Infect 15 Suppl 7, 1016 (2009)

    • Search Google Scholar
    • Export Citation
  • 11.

    Hogan B , Rakotozandrindrainy R, Al-Emran H, Dekker D, Hahn A, Jaeger A, Poppert S, Frickmann H, Hagen RM, Micheel V, Crusius S, Heriniaina JN, Rakotondrainiarivelo JP, Razafindrabe T, May J, Schwarz NG: Prevalence of nasal colonisation by methicillin-sensitive and methicillinresistant Staphylococcus aureus among healthcare workers and students in Madagascar. BMC Infect Dis 16, 420 (2016)

    • Search Google Scholar
    • Export Citation
  • 12.

    Tan TY , Ng LS, Sim DM, Cheng Y, Min MO: Evaluation of bacterial recovery and viability from three different swab transport systems. Pathology 46, 230233 (2014)

    • Search Google Scholar
    • Export Citation
  • 13.

    Micheel V , Hogan B, Köller T, Warnke P, Crusius S, Hinz R, Hagen RM, Schwarz NG, Frickmann H: Screening agars for MRSA: evaluation of a stepwise diagnostic approach with two different selective agars for the screening for methicillin-resistant Staphylococcus aureus (MRSA). Mil Med Res 2, 18 (2015)

    • Search Google Scholar
    • Export Citation
  • 14.

    Shenoy ES , Kim J, Rosenberg ES, Cotter JA, Lee H, Walensky RP, Hooper DC: Discontinuation of contact precautions for methicillin-resistant Staphylococcus aureus: a randomized controlled trial comparing passive and active screening with culture and polymerase chain reaction. Clin Infect Dis 57, 176184 (2013)

    • Search Google Scholar
    • Export Citation
  • 15.

    Shenoy ES , Lee H, Cotter JA, Ware W, Kelbaugh D, Weil E, Walensky RP, Hooper DC: Impact of rapid screening for discontinuation of methicillin-resistant Staphylococcus aureus contact precautions. Am J Infect Control 44, 215221 (2016)

    • Search Google Scholar
    • Export Citation
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Senior editors

Editor(s)-in-Chief: Dunay, Ildiko Rita

Editor(s)-in-Chief: Heimesaat, Markus M.

Vice Editor(s)-in-Chief: Fuchs, Anja

Editorial Board

Chair of the Editorial Board:
Jeffrey S. Buguliskis (Thomas Jefferson University, USA)

  • Jörn Albring (University of Münster, Germany)
  • Stefan Bereswill (Charité - University Medicine Berlin, Germany)
  • Dunja Bruder (University of Megdeburg, Germany)
  • Jan Buer (University of Duisburg, Germany)
  • Jeff Buguliskis (Thomas Jefferson University, USA)
  • Edit Buzas (Semmelweis University, Hungary)
  • Charles Collyer (University of Sydney, Australia)
  • Renato Damatta (UENF, Brazil)
  • Ivelina Damjanova (Semmelweis University, Hungary)
  • Maria Deli (Biological Research Center, HAS, Hungary)
  • Olgica Djurković-Djaković (University of Belgrade, Serbia)
  • Jean-Dennis Docquier (University of Siena, Italy)
  • Anna Erdei (Eötvös Loránd University, Hungary)
  • Zsuzsanna Fabry (University of Washington, USA)
  • Beniam Ghebremedhin (Witten/Herdecke University, Germany)
  • Nancy Guillen (Institute Pasteur, France)
  • Georgina L. Hold (University of Aberdeen, United Kingdom)
  • Ralf Ignatius (Charité - University Medicine Berlin, Germany)
  • Zsuzsanna Izsvak (MDC-Berlin, Germany)
  • Achim Kaasch (University of Cologne, Germany)
  • Tamás Laskay (University of Lübeck, Germany)
  • Oliver Liesenfeld (Roche, USA)
  • Shreemanta Parida (Vaccine Grand Challenge Program, India)
  • Matyas Sandor (University of Wisconsin, USA)
  • Ulrich Steinhoff (University of Marburg, Germany)
  • Michal Toborek (University of Miami, USA)
  • Mary Jo Wick (University of Gothenburg, Sweden)
  • Susanne A. Wolf (MDC-Berlin, Germany)

 

Dr. Dunay, Ildiko Rita
Magdeburg, Germany
E-mail: ildikodunay@gmail.com

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European Journal of Microbiology and Immunology
Language English
Size A4
Year of
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2011
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2021 Volume 11
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1
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ISSN 2062-509X (Print)
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