In the past, the horizontal transfer of antimicrobial resistance genes was mainly associated with conjugative plasmids or transposons, whereas transduction by bacteriophages was thought to be a rare event. In order to analyze the likelihood of transduction of antimicrobial resistance in the field of clinical veterinary medicine, we isolated phages from Escherichia coli from a surgery suite of an equine clinic. In a pilot study, the surgery suite of a horse clinic was sampled directly after surgery and subsequently sampled after cleaning and disinfection following a sampling plan based on hygiene, surgery, and anesthesia. In total, 31 surface sampling sites were defined and sampled. At 24 of these 31 surface sampling sites, coliphages were isolated. At 12 sites, coliphages were found after cleaning and disinfection. Randomly selected phages were tested for their ability of antimicrobial resistance transduction. Ten of 31 phages were detected to transfer antimicrobial resistance. These phages most often transduced resistance to streptomycin, encoded by the addA1 gene (n = 9), followed by resistance to chloramphenicol by cmlA (n = 3) and ampicillin (n = 1). This is, to the best of our knowledge, the first report on antimicrobial resistance-transferring bacteriophages that have been isolated at equine veterinary clinics.
Walther B , Janssen T, Gehlen H, Vincze S, Borchers K, Wieler L, Barton A, Lübke-Becker A: Infection control and hygiene management in equine hospitals. Berl Munch Tierarztl Wochenschr 127, 486–497 (2014)
Isgren CM , Salem SE, Archer DC, Worsman FCF, Townsend NB: Risk factors for surgical site infection following laparotomy: Effect of season and perioperative variables and reporting of bacterial isolates in 287 horses. Equine Vet J 49, 39–44 (2017)
Galuppo LD , Pascoe JR, Jang SS, Willits NH, Greenman SL: Evaluation of iodophor skin preparation techniques and factors influencing drainage from ventral midline incisions in horses. J Am Vet Med Assoc 215, 963–969 (1999)
Walther B , Lübke-Becker A, Stamm I, Gehlen H, Barton A, Janssen T, Wieler L, Guenther S: Suspected nosocomial infections with multi-drug resistant E. coli, including extended-spectrum beta-lactamase (ESBL)-producing strains, in an equine clinic. Berl Munch Tierarztl Wochenschr 127, 421–427 (2014)
Ahmed MO , Williams NJ, Clegg PD, van Velkinburgh JC, Baptiste KE, Bennett M: Analysis of risk factors associated with antibiotic-resistant Escherichia coli. Microb Drug Resist 18, 161–168 (2012)
Racklyeft D , Love D: Bacterial infection of the lower respiratory tract in 34 horses. Aust Vet J 78, 549–559 (2000)
Johns IC , Adams EL: Trends in antimicrobial resistance in equine bacterial isolates: 1999–2012. Vet Rec 176, 334 (2015)
Boerlin P , Reid-Smith RJ: Antimicrobial resistance: its emergence and transmission. Anim Health Res Rev 9, 115–126 (2008)
Normark BH , Normark S: Evolution and spread of antibiotic resistance. J Intern Med 252, 91–106 (2002)
Kenzaka T , Tani K, Nasu M: High-frequency phage-mediated gene transfer in freshwater environments determined at single-cell level. ISME J 4, 648–659 (2010)
Muniesa M , Colomer-Lluch M, Jofre J: Could bacteriophages transfer antibiotic resistance genes from environmental bacteria to human-body associated bacterial populations? Mob Genet Elements 3, e25847 (2013)
Shousha A , Awaiwanont N, Sofka D, Smulders FJ, Paulsen P, Szostak MP, Humphrey T, Hilbert F: Bacteriophages isolated from chicken meat and the horizontal transfer of antimicrobial resistance genes. Appl Environ Microbiol 81, 4600–4606 (2015)
Soucy SM , Huang J, Gogarten JP: Horizontal gene transfer: building the web of life. Nat Rev Genetics 16, 472–482 (2015)
Furusawa T , Iwano H, Hiyashimizu Y, Matsubara K, Higuchi H, Nagahata H, Niwa H, Katayama Y, Kinoshita Y, Hagiwara K, Iwasaki T, Tanji Y, Yokota H, Tamura Y: Phage therapy is effective in a mouse model of bacterial equine keratitis. Appl Environ Microbiol 82, 5332–5339 (2016)
Abedon ST , Kuhl SJ, Blasdel BG, Kutter EM: Phage treatment of human infections. Bacteriophage 1, 66–85 (2011)
ISO, N. ISO 10705-2 (2000): Water quality – detection and enumeration of bacteriophages Part 2: enumeration of somatic coliphages, 11–12.
Sullivan MB , Coleman ML, Weigele P, Rohwer F, Chisholm SW: Three Prochlorococcus cyanophage genomes: signature features and ecological interpretations. PLoS Biol 3, s144 (2005)
ISO, N ISO 20776-1 (2006): Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. – Part 1 Reference method for testing the invitro acitivity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases.
Williams A , Christley RM, McKane SA, Roberts VL, Clegg PD, Williams NJ: Antimicrobial resistance changes in enteric Escherichia coli of horses during hospitalisation: resistance profiling of isolates. Vet J 195, 121–126 (2013)
Langsrud S , Sundheim G, Borgmann-Strahsen R: Intrinsic and acquired resistance to quaternary ammonium compounds in food-related Pseudomonas spp. J Appl Microbiol 95, 874–882 (2003)
Shousha A , Paulsen P, Sofka D, Hilbert M, Dinhopl N, Hilbert F: Tenazität von antibiotikaresistenzübertragenden Bakteriophagen. 16. Fachtagung für Fleisch- und Geflügelfleischhygiene. 1.-2. März, Berlin (2016)
Maidhof H , Guerra B, Abbas S, Elsheikha HM, Whittam TS, Beutin L: A multiresistant clone of shiga toxin-producing Escherichia coli O118: (H16) is spread in cattle and humans over different European countries. Appl Environ Microbiol 68, 5834–5842 (2002)
Chen S , Zhao S, White DG, Schroeder CM, Lu R, Yang H, McDermott PF, Ayers S, Meng J: Characterization of multiple- antimicrobial-resistant Salmonella serovars isolated from retail meats. Appl Environ Microbiol 70, 1–7 (2004)
Van TT , Chin J, Chapman T, Tran LT, Coloe PJ: Safety of raw meat and shellfish in Vietnam: an analysis of Escherichia coli isolations for antibiotic resistance and virulence genes. Int J Food Microbiol 124, 217–223 (2008)