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  • 1 Semmelweis Egyetem, Általános Orvostudományi Kar II. Belgyógyászati Klinika Budapest Szentkirályi u. 46. 1088
  • 2 Semmelweis Egyetem, Általános Orvostudományi Kar I. Gyerekgyógyászati Klinika Budapest
  • 3 EGIS Gyógyszergyár Nyrt. Budapest
  • 4 Magyar Tudományos Akadémia Molekuláris Medicina Kutatócsoport Budapest
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A vastagbél-biopsziák nagy teljesítményű oligonukleotid microarray-vizsgálata segítségünkre lehet a helyi patofiziológiai eltérések megértésében, valamint elősegítheti a colorectalis adenomák, karcinómák és gyulladásos bélbetegségek funkcionális klasszifikációját. Módszerek: 15 vastagbélrákos, 15 adenomás, 14 gyulladásos bélbetegségben szenvedő beteg biopsziás mintájából teljes ribonukleinsav izolálását, amplifikációját és biotinos jelölését végeztük. A teljes genomszintű génexpressziós mintázat meghatározása Human Genome U133 Plus 2.0 microarray-ken történt. Két független normalizációs módszert követően a diagnosztikus génmintázat meghatározására „Prediction Analysis of Microarrays” módszert használtunk. Leave one-out lépésenkénti diszkriminanciaelemzést végeztünk. Az expressziós eredményeket valós idejű polimeráz láncreakcióval igazoltuk. Eredmények: Adenomában a „top” igazolt gének a következők voltak: CD44-antigén, met proto-onkogén, kemokin ligand-12, ADAM-szerű decizin-1 és az ATP-kötő kazetta-A8; vastagbélrákban a kollagén-IVα1, lipokalin-2, kalumenin, akvaporin-8; és gyulladásos bélbetegségben a lipokalin-2, ubikvitin D és az interferon indukálta transzmembrán-fehérje-2. A diszkriminanciaelemzéssel kapott elkülönítő gének expressziója alapján átlagosan 96,2%-os pontossággal csoportosíthatók a minták. A Taqman valós idejű polimeráz láncreakcióval vizsgált, 52 kiválasztott gén 94%-ának expressziós szintje szignifikánsan korrelált az Affymetrix microarray vizsgálatban kapott eredményekkel ( p < 0,05). Következtetések: Biopsziás minták felhasználásával sikeresen végeztünk teljes genomszintű expressziós microarray-vizsgálatot, amely alkalmasnak bizonyult elkülönítő génmintázatok azonosítására. Eredményeink további elemzésekre felhasználható génexpressziós adattárat biztosítanak.

  • Galamb, O., Sipos, F., Fischer, K. és mtsai: The results of the expression array studies correlate and enhance the known genetic basis of gastric and colorectal cancer. Cytometry B. Clin. Cytom., 2005, 68 , 1–17.

    Fischer K. , 'The results of the expression array studies correlate and enhance the known genetic basis of gastric and colorectal cancer ' (2005 ) 68 Cytometry B. Clin. Cytom. : 1 -17.

    • Search Google Scholar
  • Kitahara, O., Furukawa, Y., Tanaka, T. és mtsai: Alterations of gene expression during colorectal carcinogenesis revealed by cDNA microarrays after laser-capture microdissection of tumor tissues and normal epithelia. Cancer Res., 2001, 61 , 3544–3549.

    Tanaka T. , 'Alterations of gene expression during colorectal carcinogenesis revealed by cDNA microarrays after laser-capture microdissection of tumor tissues and normal epithelia ' (2001 ) 61 Cancer Res. : 3544 -3549.

    • Search Google Scholar
  • Notterman, D. A., Alon, U., Sierk, A. J. és mtsa: Transcriptional gene expression profiles of colorectal adenoma, adenocarcinoma, and normal tissue examined by oligonucleotide arrays. Cancer Res., 2001, 61 , 3124–3130.

    Sierk A. J. , 'Transcriptional gene expression profiles of colorectal adenoma, adenocarcinoma, and normal tissue examined by oligonucleotide arrays ' (2001 ) 61 Cancer Res. : 3124 -3130.

    • Search Google Scholar
  • Croner, R. S., Foertsch, T., Brueckl, W. M. és mtsai: Common denominator genes that distinguish colorectal carcinoma from normal mucosa. Int. J. Colorectal. Dis., 2005, 20 , 353–362.

    Brueckl W. M. , 'Common denominator genes that distinguish colorectal carcinoma from normal mucosa ' (2005 ) 20 Int. J. Colorectal. Dis. : 353 -362.

    • Search Google Scholar
  • Kwon, H. C., Kim, S. H., Roh, M. S. és mtsai: Gene expression profiling in lymph node-positive and lymph node-negative colorectal cancer. Dis. Col. Rect., 2004, 47 , 141–152.

    Roh M. S. , 'Gene expression profiling in lymph node-positive and lymph node-negative colorectal cancer ' (2004 ) 47 Dis. Col. Rect. : 141 -152.

    • Search Google Scholar
  • Agrawal, D., Chen, T., Irby, R. és mtsai: Osteopontin identified as colon cancer tumor progression marker. C. R. Biol., 2003, 326 , 1041–1043.

    Irby R. , 'Osteopontin identified as colon cancer tumor progression marker ' (2003 ) 326 C. R. Biol. : 1041 -1043.

    • Search Google Scholar
  • Bandres, E., Catalan, V., Sola, I. és mtsai: Dysregulation of apoptosis is a major mechanism in the lymph node involvement in colorectal carcinoma. J. Oncol. Rep., 2004, 12 , 287–292.

    Sola I. , 'Dysregulation of apoptosis is a major mechanism in the lymph node involvement in colorectal carcinoma ' (2004 ) 12 J. Oncol. Rep. : 287 -292.

    • Search Google Scholar
  • Bertucci, F., Salas, S., Eysteries, S. és mtsai: Gene expression profiling of colon cancer by DNA microarrays and correlation with histoclinical parameters. Oncogene, 2004, 23 , 1377–1391.

    Eysteries S. , 'Gene expression profiling of colon cancer by DNA microarrays and correlation with histoclinical parameters ' (2004 ) 23 Oncogene : 1377 -1391.

    • Search Google Scholar
  • Birkenkamp-Demtroder, K., Christensen, L. L., Olesen, S. H. és mtsai: Gene expression in colorectal cancer. Cancer Res., 2002, 62 , 4352–4363.

    Olesen S. H. , 'Gene expression in colorectal cancer ' (2002 ) 62 Cancer Res. : 4352 -4363.

  • Li, M., Lin, Y. M., Hasegawa, S. és mtsai: Genes associated with liver metastasis of colon cancer, identified by genome-wide cDNA microarray. Int. J. Oncol., 2004, 24 , 305–312.

    Hasegawa S. , 'Genes associated with liver metastasis of colon cancer, identified by genome-wide cDNA microarray ' (2004 ) 24 Int. J. Oncol. : 305 -312.

    • Search Google Scholar
  • Yanagawa, R., Furukawa, Y., Tsunoda, T. és mtsai: Genome-wide screening of genes showing altered expression in liver metastases of human colorectal cancers by cDNA microarray. Neoplasia, 2001, 3 , 395–401.

    Tsunoda T. , 'Genome-wide screening of genes showing altered expression in liver metastases of human colorectal cancers by cDNA microarray ' (2001 ) 3 Neoplasia : 395 -401.

    • Search Google Scholar
  • Williams, N. S., Gaynor, R. B., Scoggin, S. és mtsai: Identification and validation of genes involved in the pathogenesis of colorectal cancer using cDNA microarrays and RNA interference. Clin. Cancer Res., 2003, 9 , 931–946.

    Scoggin S. , 'Identification and validation of genes involved in the pathogenesis of colorectal cancer using cDNA microarrays and RNA interference ' (2003 ) 9 Clin. Cancer Res. : 931 -946.

    • Search Google Scholar
  • Lin, Y. M., Furukawa, Y., Tsunoda, T. és mtsai: Molecular diagnosis of colorectal tumors by expression profiles of 50 genes expressed differentially in adenomas and carcinomas. Oncogene, 2002, 21 , 4120–4128.

    Tsunoda T. , 'Molecular diagnosis of colorectal tumors by expression profiles of 50 genes expressed differentially in adenomas and carcinomas ' (2002 ) 21 Oncogene : 4120 -4128.

    • Search Google Scholar
  • Zou, T. T., Selaru, F. M., Xu, Y. és mtsai: Application of cDNA microarrays to generate a molecular taxonomy capable of distinguishing between colon cancer and normal colon. Oncogene, 2002, 21 , 4855–4862.

    Xu Y. , 'Application of cDNA microarrays to generate a molecular taxonomy capable of distinguishing between colon cancer and normal colon ' (2002 ) 21 Oncogene : 4855 -4862.

    • Search Google Scholar
  • Frederiksen, C. M., Knudsen, S., Laurberg, S. és mtsa: Classification of Dukes’ B and C colorectal cancers using expression arrays. J. Cancer Res. Clin. Oncol., 2003, 129 , 263–271.

    Laurberg S. , 'Classification of Dukes’ B and C colorectal cancers using expression arrays ' (2003 ) 129 J. Cancer Res. Clin. Oncol. : 263 -271.

    • Search Google Scholar
  • Birkenkamp-Demtroder, K., Olesen, S. H., Sorensen, F. B. és mtsai: Differential gene expression in colon cancer of the caecum versus the sigmoid and rectosigmoid. Gut, 2005, 54 , 374–384.

    Sorensen F. B. , 'Differential gene expression in colon cancer of the caecum versus the sigmoid and rectosigmoid ' (2005 ) 54 Gut : 374 -384.

    • Search Google Scholar
  • Chiu, S. T., Hsieh, F. J., Chen, S. W. és mtsai: Clinicopathologic correlation of up-regulated genes identified using cDNA microarray and real-time reverse transcription-PCR in human colorectal cancer. Cancer Epidemiol. Biomarkers Prev., 2005, 14 , 437–443.

    Chen S. W. , 'Clinicopathologic correlation of up-regulated genes identified using cDNA microarray and real-time reverse transcription-PCR in human colorectal cancer ' (2005 ) 14 Cancer Epidemiol. Biomarkers Prev. : 437 -443.

    • Search Google Scholar
  • Wang, Y., Jatkoe, T., Zhang, Y. és mtsai: Gene expression profiles and molecular markers to predict recurrence of Dukes’ B colon cancer. J. Clin. Oncol., 2004, 22 , 1564–1571.

    Zhang Y. , 'Gene expression profiles and molecular markers to predict recurrence of Dukes’ B colon cancer ' (2004 ) 22 J. Clin. Oncol. : 1564 -1571.

    • Search Google Scholar
  • Langmann, T., Moehle, C., Mauerer, R. és mtsai: Loss of detoxification in inflammatory bowel disease: dysregulation of pregnane X receptor target genes. Gastroenterology, 2004, 127 , 26–40.

    Mauerer R. , 'Loss of detoxification in inflammatory bowel disease: dysregulation of pregnane X receptor target genes ' (2004 ) 127 Gastroenterology : 26 -40.

    • Search Google Scholar
  • Lawrance, I. C., Fiocchi, C., Chakravarti, S.: Ulcerative colitis and Crohn’s disease: distinctive gene expression profiles and novel susceptibility candidate genes. Hum. Mol. Genet., 2001, 10 , 445–456.

    Chakravarti S. , 'Ulcerative colitis and Crohn’s disease: distinctive gene expression profiles and novel susceptibility candidate genes ' (2001 ) 10 Hum. Mol. Genet. : 445 -456.

    • Search Google Scholar
  • Okahara, S., Arimura, Y., Yabana, T. és mtsai: Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis. Aliment. Pharmacol. Ther., 2005, 21 , 1091–1097.

    Yabana T. , 'Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis ' (2005 ) 21 Aliment. Pharmacol. Ther. : 1091 -1097.

    • Search Google Scholar
  • Uthoff, S. M., Eichenberger, M. R., Lewis, R. K. és mtsai: Identification of candidate genes in ulcerative colitis and Crohn’s disease using cDNA array technology. Int. J. Oncol., 2001, 19 , 803–810.

    Lewis R. K. , 'Identification of candidate genes in ulcerative colitis and Crohn’s disease using cDNA array technology ' (2001 ) 19 Int. J. Oncol. : 803 -810.

    • Search Google Scholar
  • Puleston, J., Cooper, M., Murch, S. és mtsai: A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease. Aliment. Pharmacol. Ther., 2005, 21 , 109–120.

    Murch S. , 'A distinct subset of chemokines dominates the mucosal chemokine response in inflammatory bowel disease ' (2005 ) 21 Aliment. Pharmacol. Ther. : 109 -120.

    • Search Google Scholar
  • Mannick, E. E., Bonomolo, J. C., Horswell, R. és mtsai: Gene expression in mononuclear cells from patients with inflammatory bowel disease. Clin. Immunol., 2004, 112 , 247–257.

    Horswell R. , 'Gene expression in mononuclear cells from patients with inflammatory bowel disease ' (2004 ) 112 Clin. Immunol. : 247 -257.

    • Search Google Scholar
  • Galamb, O., Sipos, F., Dinya, E. és mtsai: mRNA expression, functional profiling and multivariate classification of colon biopsy specimen by cDNA overall glass microarray. World J. Gastroenterol., 2006, 12 , 6998–7006.

    Dinya E. , 'mRNA expression, functional profiling and multivariate classification of colon biopsy specimen by cDNA overall glass microarray ' (2006 ) 12 World J. Gastroenterol. : 6998 -7006.

    • Search Google Scholar
  • Van Gelder, R. N., von Zastrow, M. E., Yool, A. és mtsai: Amplified RNA synthesized from limited quantities of heterogenous cDNA. Proc. Natl. Acad. Sci. USA, 1990, 87 , 1663–1667.

    Yool A. , 'Amplified RNA synthesized from limited quantities of heterogenous cDNA ' (1990 ) 87 Proc. Natl. Acad. Sci. USA : 1663 -1667.

    • Search Google Scholar
  • Tumor Analysis Best Practices Working Group: Expression profiling-best practices for data generation and interpretation in clinical trials. Nat. Rev. Genet., 2004, 5 , 229–237.

    'Expression profiling-best practices for data generation and interpretation in clinical trials ' (2004 ) 5 Nat. Rev. Genet. : 229 -237.

    • Search Google Scholar
  • Irizarry, R. A., Gautier, L., Cope, L. M.: The Analysis of Gene Expression Data: Methods and Software, chapter 4. Springer Verlag, 2003.

  • Tibshirani, R., Hastie, T., Narasimhan, B. és mtsa: Diagnosis of multiple cancer types by shrunken centroids of gene expression. Proc. Natl. Acad. Sci. USA, 2002, 99 , 6567–6572.

    Narasimhan B. , 'Diagnosis of multiple cancer types by shrunken centroids of gene expression ' (2002 ) 99 Proc. Natl. Acad. Sci. USA : 6567 -6572.

    • Search Google Scholar
  • Fischer, H., Stenling, R., Rubio, C. és mtsa: Differential expression of aquaporin 8 in human colonic epithelial cells and colorectal tumors. BMC Physiol., 2001, 1 , 1.

    Rubio C. , 'Differential expression of aquaporin 8 in human colonic epithelial cells and colorectal tumors ' (2001 ) 1 BMC Physiol. : 1 -.

    • Search Google Scholar
  • Trovato, M., Vitarelli, E., Grosso, M. és mtsai: Immunohistochemical expression of HGF, c-MET and transcription factor STAT3 in colorectal tumors. Eur. J. Histochem., 2004, 48 , 291–297.

    Grosso M. , 'Immunohistochemical expression of HGF, c-MET and transcription factor STAT3 in colorectal tumors ' (2004 ) 48 Eur. J. Histochem. : 291 -297.

    • Search Google Scholar
  • Cohen, M. B., Hawkins, J. A., Witte, D. P.: Guanylin mRNA expression in human intestine and colorectal adenocarcinoma. Lab. Invest., 1998, 78 , 101–108.

    Witte D. P. , 'Guanylin mRNA expression in human intestine and colorectal adenocarcinoma ' (1998 ) 78 Lab. Invest. : 101 -108.

    • Search Google Scholar
  • Shibuta, K., Begum, N. A., Mori, M. és mtsai: Reduced expression of the CXC chemokine hIRH/SDF-1alpha mRNA in hepatoma and digestive tract cancer. Int. J. Cancer, 1997, 73 , 656–662.

    Mori M. , 'Reduced expression of the CXC chemokine hIRH/SDF-1alpha mRNA in hepatoma and digestive tract cancer ' (1997 ) 73 Int. J. Cancer : 656 -662.

    • Search Google Scholar
  • Dwinell, M. B., Ogawa, H., Barrett, K. E. és mtsa: SDF-1/CXCL12 regulates cAMP production and ion transport in intestinal epithelial cells via CXCR4. Am. J. Physiol. Gastrointest. Liver Physiol., 2004, 286 , G844–G850.

    Barrett K. E. , 'SDF-1/CXCL12 regulates cAMP production and ion transport in intestinal epithelial cells via CXCR4 ' (2004 ) 286 Am. J. Physiol. Gastrointest. Liver Physiol. : G844 -G850.

    • Search Google Scholar
  • Hisamatsu, T., Watanabe, M., Ogata, H. és mtsai: Interferon-inducible gene family 1-8U expression in colitis-associated colon cancer and severely inflamed mucosa in ulcerative colitis. Cancer Res., 1999, 59 , 5927–5931.

    Ogata H. , 'Interferon-inducible gene family 1-8U expression in colitis-associated colon cancer and severely inflamed mucosa in ulcerative colitis ' (1999 ) 59 Cancer Res. : 5927 -5931.

    • Search Google Scholar
  • Dooley, T. P., Curto, E. V., Reddy, S. P. és mtsai: Regulation of gene expression in inflammatory bowel disease and correlation with IBD drugs: screening by DNA microarrays. Inflamm. Bowel Dis., 2004, 10 , 1–14.

    Reddy S. P. , 'Regulation of gene expression in inflammatory bowel disease and correlation with IBD drugs: screening by DNA microarrays ' (2004 ) 10 Inflamm. Bowel Dis. : 1 -14.

    • Search Google Scholar

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