Daidzein affects steroidogenesis and oestrogen receptor expression in medium ovarian follicles of pigs

Anna Nynca; Dominika Słonina; Olga Jablońska; Barbara Kamińska; Renata Ciereszko

doi:10.1556/avet.2012.060

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Acta Veterinaria Hungarica

Volume/Issue:: Volume 61: Issue 1

Daidzein affects steroidogenesis and oestrogen receptor expression in medium ovarian follicles of pigs

Authors:

Anna Nynca

Anna Nynca

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Dominika Słonina

Dominika Słonina Polish Academy of Sciences Department of Cattle Reproduction Endocrinology, Institute of Animal Reproduction and Food Research Olsztyn Poland

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Olga Jablońska

Olga Jablońska University of Warmia and Mazury Department of Animal Physiology Olsztyn Poland

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Barbara Kamińska

Barbara Kamińska University of Warmia and Mazury Department of Animal Physiology Olsztyn Poland

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Renata Ciereszko

Renata Ciereszko

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Pages:: 85–98

Online Publication Date:: 06 Dec 2012

Publication Date:: 01 Mar 2013

Article Category:: Journal Article

DOI:: https://doi.org/10.1556/avet.2012.060

Keywords:: Phytoestrogen; daidzein; steroidogenesis; oestrogen receptor

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Daidzein, a phytoestrogen present in soybean products used in swine feed, has been demonstrated to affect both reproductive and endocrine functions. The aims of this study were to examine the in vitro effects of daidzein on (1) progesterone (P₄) and oestradiol (E₂) secretion by porcine luteinised granulosa cells harvested from medium follicles, and (2) the mRNA and protein expression of oestrogen receptors α and β (ERα and ERβ) in these cells. The influence of E₂ on P₄ secretion and ERα and ERβ expression in the granulosa cells of pigs was also investigated. It was found that daidzein inhibited progesterone secretion by luteinised granulosa cells isolated from medium follicles. In contrast, E₂ did not affect progesterone production by these cells. Moreover, daidzein did not alter the granulosal secretion of E₂. Both daidzein and E₂ decreased mRNA expression of ERα in the cells examined. The expression of ERβ mRNA was not affected by daidzein but was inhibited by E₂. ERα protein was not detected while ERβ protein was found in the nuclei of the cells. Daidzein and E₂ upregulated the expression of ERβ protein in the cells. In summary, the phytoestrogen daidzein directly affected the porcine ovary by inhibiting progesterone production and increasing ERβ protein expression. Daidzein-induced changes in follicular steroidogenesis and granulosal sensitivity to oestrogens may disturb reproductive processes in pigs.

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Acta Veterinaria Hungarica

Print ISSN:: 0236-6290

Online ISSN:: 1588-2705

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Editorial Board

Mária BENKŐ (Acta Veterinaria Hungarica, Budapest, Hungary)
Gábor BODÓ (University of Veterinary Medicine, Budapest, Hungary)
Béla DÉNES (University of Veterinary Medicine, Budapest Hungary)
Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
Hedvig FÉBEL (University of Veterinary Medicine, Budapest, Hungary)
László FODOR (University of Veterinary Medicine, Budapest, Hungary)
János GÁL (University of Veterinary Medicine, Budapest, Hungary)
Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
Dušan PALIĆ (Ludwig Maximilian University, Munich, Germany)
Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
János VARGA (University of Veterinary Medicine, Budapest, Hungary)
Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

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Acta Veterinaria Hungarica
Language	English
Size	A4
Year of Foundation	1951
Volumes per Year	1
Issues per Year	4
Founder	Magyar Tudományos Akadémia
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ISSN	0236-6290 (Print)
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