Acta Biologica Hungarica
Volume/Issue:
Volume 60: Issue 2
Carnosine attenuates oxidative stress and apoptosis in transient cerebral ischemia in rats
Authors:
C. Pekcetin Dokuz Eylul University Medical School Department of Histology & Embryology Balcova, Izmir 35340 Turkey

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Muge Kiray Dokuz Eylul University Medical School Department of Histology & Embryology Balcova, Izmir 35340 Turkey

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B. Ergur Dokuz Eylul University Medical School Department of Histology & Embryology Balcova, Izmir 35340 Turkey

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K. Tugyan Dokuz Eylul University Medical School Department of Histology & Embryology Balcova, Izmir 35340 Turkey

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H. Bagriyanik Dokuz Eylul University Medical School Department of Histology & Embryology Balcova, Izmir 35340 Turkey

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G. Erbil Dokuz Eylul University Medical School Department of Histology & Embryology Balcova, Izmir 35340 Turkey

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Basak Baykara Dokuz Eylul University Medical School Department of Histology & Embryology Balcova, Izmir 35340 Turkey

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U. Camsari Dokuz Eylul University Medical School Department of Physiology Balcova, Izmir 35340 Turkey

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Pages:
137–148
Online Publication Date:
07 Jul 2009
Publication Date:
01 Jun 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.60.2009.2.1
Keywords:
Carnosine; ischemia-reperfusion; oxidative stress; apoptosis; spatial memory
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Cerebral ischemia leads to cognitive decline and neuronal damage in the hippocampus. Reactive oxygen species (ROS) play an important role in the neuronal loss after cerebral ischemia and reperfusion injury. Carnosine has both antioxidant and neuroprotective effects against ROS. In the present study, the effects of carnosine on oxidative stress, apoptotic neuronal cell death and spatial memory following transient cerebral ischemia in rats were investigated. Transient ischemia was induced by occlusion of right common carotid artery of rats for 30 min and reperfusion for 24 h or 1 week. Rats received intraperitoneal injection of 250 mg/kg carnosine or saline 30 min prior to experiment. Determination of antioxidant enzyme activities was performed spectrophotometrically. To detect apoptotic cells, TUNEL staining was performed using an In Situ Cell Death Detection Kit. Carnosine treatment elicited a significant decrease in lipid peroxidation and increase in antioxidant enzyme activities in ischemic rat brains. The number of TUNEL-positive cells was decreased significantly in carnosine-treated group when compared with the ischemia-induction group. Carnosine treatment did not provide significant protection from ischemia induced deficits in spatial learning. The results show that carnosine is effective as a prophylactic treatment for brain tissue when it is administered before ischemia without affecting spatial memory.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Founder Magyar Tudományos Akadémia
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