Authors:
Vladimir Ajdžanović Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

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V Jakovljević Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, Kragujevac, Serbia

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D Milenković INRA, UMR1019, UNH, CRNH Auvergne, Clermont-Ferrand, Unité de Nutrition Humaine, Clermont Université, Université d’Auvergne, Clermont-Ferrand, France

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A Konić-Ristić Center of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, Belgrade, Serbia

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J Živanović Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

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I Jarić Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

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V Milošević Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

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Purpose: Deformability/rheologic behavior of erythrocytes are related to near-surface membrane fluidity. Specific agents can increase erythrocyte membrane fluidity in order to adjust hemodynamics in cardiovascular diseases. Grapefruit flavanone naringenin has been proposed for potential use in an alternative therapy of cardiovascular conditions. In respect to this, we assessed here effects of two nutritionally relevant concentrations of naringenin (0.1 and 1 μg/ml) on near-surface membrane fluidity in human erythrocytes. Methods: We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity. Results: The results showed a significant (p = 0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher concentration of naringenin. In the deeper layer, just below the erythrocyte membrane phospholipid heads, both lower and higher concentration of naringenin significantly increased membrane fluidity (p = 0.036 and p = 0.028, respectively). Conclusions: These data document the positive and dose dependent effect of naringenin on near-surface membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics.

  • 1.

    Ajdžanović V , Spasojević I, Filipović B, Šošić-Jurjević B, Sekulić M, Milošević V: Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. Can. J. Physiol. Pharmacol. 88, 497500 (2010)

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  • 2.

    Ajdžanović V , Spasojević I, Šošić-Jurjević B, Filipović B, Trifunović S, Sekulić M, Milošević V: The negative effect of soy extract on erythrocyte membrane fluidity: an electron paramagnetic study. J. Membrane. Biol. 239. 131135 (2011)

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  • 3.

    Ajdžanović V , Spasojević I, Pantelić J, Šošić-Jurjević B, Filipović B, Milošević V, Severs W: Vitex agnus-castus L. essential oil increases human erythrocyte membrane fluidity. J. Med. Biochem. 31, 222227 (2012)

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    Ajdžanović V , Medigović I, Pantelić J, Milošević V: Soy isoflavones and cellular mechanics. J. Bioenerg. Biomembr. 46, 99107 (2014)

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    Asnacios A , Hamant O: The mechanics behind cell polarity. Trends Cell. Biol. 22, 584591 (2012)

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    Chanet A , Milenkovic D, Manach C, Mazur A, Morand C: Citrus flavanones: what is their role in cardiovascular protection? J. Agric. Food Chem. 60, 88098822 (2012)

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    Cho KW , Kim YO, Andrade JE, Burgess JR, Kim YC: Dietary naringenin increases hepatic peroxisome proliferators-activated receptor alfa protein expression and decreases plasma triglyceride and adiposity in rats. Eur. J. Nutr. 50, 8188 (2011)

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    Erlund I , Meririnne E, Alfthan G, Aro A: Plasma kinetics and urinary excretion of the flavanones naringenin and hesperetin in humans after ingestion of orange juice and grapefruit juice. J. Nutr. 131, 235241 (2001)

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    Funari SS , Barcelo F, Escriba PV: Effects of oleic acid and its congeners, elaidic and staric acids, on the structural properties of phosphatidylethanolamine membranes. J. Lipid Res. 44, 567575 (2003)

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    Lee CH , Jeong TS, Choi YK, Hyun BH, Oh GT, Kim EH, Kim JR, Han JI, Bok SH: Anti-atherogenic effect of citrus flavonoids, naringin and naringenin, associated with hepatic ACAT and aortic VCAM-1 and MCP-1 in high cholesterol-fed rabbits. Biochem. Biophys. Res. Commun. 284, 681688 (2001)

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  • 15.

    Lee MK , Bok SH, Jeong TS, Moon SS, Lee SE, Park YB, Choi MS: Supplementation of naringenin and its synthetic derivative alters antioxidant enzyme activities of erythrocyte and liver in high cholesterol-fed rats. Bioorg. Med. Chem. 10, 22392244 (2002)

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    Recourt K , van Brussel AA, Driessen AJ, Lughtenberg BJ: Accumulation of a nod gene inducer, the flavonoid naringenin, in the cytoplasmic membrane of Rhisobium leguminosarum biovarviciae is caused by the pH-dependent hydrophobicity of naringenin. J. Bacteriol. 171, 43701377 (1989)

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    Sonniez M , Ince HY, Yalcin O, Ajdžanović V, Spasojević I, Meiselman HJ, Baskurt OK: The effect of alcohols on red blood cell mechanical properties and membrane fluidity depends on their molecular size. PloS One 8, e76579 (2013)

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Senior editors

Editor(s)-in-Chief: Rosivall, László

Honorary Editor(s)-in-Chief): Monos, Emil

Managing Editor(s): Bartha, Jenő; Berhidi, Anna

Co-editor(s): Koller, Ákos; Lénárd, László; Szénási, Gábor

Assistant Editor(s): G. Dörnyei (Budapest), Zs. Miklós (Budapest), Gy. Nádasy (Budapest)

Hungarian Editorial Board

    1. Benedek, György (Szeged)
    1. Benyó, Zoltán (Budapest)
    1. Boros, Mihály (Szeged)
    1. Chernoch, László (Debrecen)
    1. Détári, László (Budapest)
    1. Hamar, János (Budapest)
    1. Hantos, Zoltán (Szeged)
    1. Hunyady, László (Budapest)
    1. Imre, Sándor (Debrecen)
    1. Jancsó, Gábor (Szeged)
    1. Karádi, Zoltán (Pécs)
    1. Kovács, László (Debrecen)
    1. Palkovits, Miklós (Budapest)
    1. Papp, Gyula (Szeged)
    1. Pavlik, Gábor (Budapest)
    1. Spät, András (Budapest)
    1. Szabó, Gyula (Szeged)
    1. Szelényi, Zoltán (Pécs)
    1. Szolcsányi, János (Pécs)
    1. Szollár, Lajos (Budapest)
    1. Szücs, Géza (Debrecen)
    1. Telegdy, Gyula (Szeged)
    1. Toldi, József (Szeged)
    1. Tósaki, Árpád (Debrecen)

International Editorial Board

    1. R. Bauer (Jena)
    1. W. Benjelloun (Rabat)
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    1. N. Westerhof (Amsterdam)
    1. L. F. Zhang (Xi'an)

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Acta Physiologica Hungarica
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Acta Physiologica Hungarica
Language English
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1950
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changed title
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Founder Magyar Tudományos Akadémia
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H-1051 Budapest, Hungary, Széchenyi István tér 9.
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H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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ISSN 0231-424X (Print)
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