Cereal Research Communications
Volume/Issue:
Volume 43: Issue 4

Evaluation of α-amylase activity and falling number around maturity for soft white and soft red wheat varieties in Michigan

Authors:
N. Yu Michigan State University, East Lansing, MI 48824, USA
University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

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R. Laurenz Michigan State University, East Lansing, MI 48824, USA

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L. Siler Michigan State University, East Lansing, MI 48824, USA

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P. K. W. Ng Michigan State University, East Lansing, MI 48824, USA

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E. Souza Soft Wheat Quality Laboratory, USDA-ARS, Wooster, OH 44691, USA
Bayer CropScience LP, Lincoln, NE 68521, USA

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J. M. Lewis Michigan State University, East Lansing, MI 48824, USA
Bayer CropScience LP, Lincoln, NE 68521, USA

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Pages:
672–681
Online Publication Date:
Dec 2015
Publication Date:
01 Dec 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/0806.43.2015.026
Keywords:
falling number; wheat; α-amylase; Michigan; pre-harvest sprouting; PHS; red wheat; white wheat; quality; breeding
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White wheat is, categorically, more susceptible to pre-harvest sprouting (PHS) than red wheat. Physiological maturity (PM), defined as when the seeds reach their maximum dry weight, is a critical time before harvesting. The objective of this study was to determine a reference level of α-amylase activity and the corresponding Falling Number (FN) value near the time of PM of selected red and white cultivars in the absence of PHS inducing conditions. Twenty-four soft winter wheat genotypes (12 red and 12 white) adapted to Michigan with varying historic levels of susceptibility to PHS were planted in an α-lattice design in two locations from 2008 to 2010. Spikes were collected three days before PM, at PM, and three days post PM. Samples were freeze-dried, threshed, milled and evaluated for α-amylase activity and FN value using high throughput method. Within genotype, clear trends were observed in the reduction of α-amylase activity and the increase of FN value during the physiological maturation. A nonlinear relationship between α-amylase activity and FN value was fit with an r2 of 0.801. Significant differences were observed for genotype for both α-amylase activity and FN value for all collection time points. No significant differences were found between red and white wheat, categorically, at any of the three time-points in the absence of PHS. The evaluation results provide a critical reference prior to induction of PHS. The α-amylase activity and FN tests show different advantages in analyzing PHS samples as the relationship between α-amylase activity and FN value is not linear over wide-ranging results.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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