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E. Skrzypek Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland

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M. Warchoł Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland

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I. Czyczyło-Mysza Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland

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I. Marcińska Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland

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A. Nowakowska Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland

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K. Dziurka Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland

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K. Juzoń Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland

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A. Noga Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland

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Oat haploid embryos were obtained by wide crossing with maize. The effect of light intensity during the growing period of donor plants (450 and 800 µmol m−2 s−1) and in vitro cultures (20, 40, 70 and 110 µmol m−2 s−1) was examined for the induction and development of oat DH lines. Oat florets (26008) from 32 genotypes were pollinated with maize and treated with 2,4-dichlorophenoxyacetic acid. All the tested genotypes formed more haploid embryos when donor plants were grown in a greenhouse (9.4%) compared to a growth chamber (6.1%). The light intensity of 110 µmol m−2 s−1 during in vitro culture resulted in the highest percentage of embryo germination (38.9%), conversion into plants (36.4%) and DH line production (9.2%) when compared with lower light intensities (20, 40 and 70 µmol m−2 s−1). The results show that the growth conditions of the donor plant and light intensity during in vitro culture can affect the development of haploid embryos. This fact may have an impact on oat breeding programs using oat × maize crosses.

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Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)