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I. Marcińska The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Cracow, Poland

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I. Czyczyło-Mysza The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Cracow, Poland

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E. Skrzypek The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Cracow, Poland

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M. Warchoł The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Cracow, Poland

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K. Zieliński The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Cracow, Poland

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E. Dubas The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Cracow, Poland

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The aim of this study was to determine the effect of selected factors on rye (Secale cereale L.) haploid embryo production by the wide crossing method. The study was performed on fifteen winter rye genotypes. This is the first time for rye when besides the genotype, on the enlargement of ovaries and haploid embryo production, such factors as: type of auxin analogues 2,4-dichlorophenoxyacetic acid (2,4-D), 3,6-dichloro-2-methoxybenzoic acid (dicamba) and 4-amino-3,5,6-trichloropyridine-2-carboxylic acid (picloram), and the time between florets emasculation and pollination were investigated. All factors had a significant impact on rye ovary enlargement, however the haploid embryo formation depended only on rye genotype, not on kind of auxin and days between emasculation to pollination. In total, twenty one haploid embryos were formed by six genotypes of fifteen tested. On average, 13.86% (after 2,4-D treatment) to 20.05% (after dicamba treatment) enlarged ovaries per emasculated florets were obtained. Most of the ovaries enlarged when florets were pollinated 4 and 6 days after emasculation. Most of the haploid embryos formed when florets were pollinated 6 days after emasculation. The obtained haploid embryos did not germinate.

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Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)