JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 16: Issue 1
Densitometric determination of embelin in Lysimachia punctata
Authors:
Irma Podolak Jagiellonian University Department of Pharmacognosy Medyczna 9 30-688 Cracow Poland

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Zbigniew Janeczko Jagiellonian University Department of Pharmacognosy Medyczna 9 30-688 Cracow Poland

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Jan Krzek Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum Medyczna 9 30-688 Cracow Poland

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Anna Apola Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum Medyczna 9 30-688 Cracow Poland

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Dorota Walusiak Jagiellonian University Department of Inorganic and Analytical Chemistry, Collegium Medicum Medyczna 9 30-688 Cracow Poland

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Pages:
48–51
Online Publication Date:
08 Mar 2007
Publication Date:
01 Feb 2003
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.16.2003.1.10
Keywords:
Embelin; Benzoquinone; Densitometry
Restricted access

A thin layer chromatographic method with densitometric UV detection at λ = 285 nm has been developed for quantification of embelin in Lysimachia punctata . TLC analysis was performed on aluminumbacked silica gel 60 F 254 plates with n -hexane-ethyl acetate, 7 + 3 ( v / v ), as mobile phase. Under these experimental conditions the method was highly sensitive (the limit of detection was 12.5 ng) and recovery was satisfactory (from 89.45% to 92.28%). Good results obtained during validation of the method were confirmed in quantification of embelin, because high precision and accuracy were achieved.

  • H. Ogawa and S. Natori , Phytochemistry 7 (1968) 773–782.

    Natori S. , '' (1968 ) 7 Phytochemistry : 773 -782 .

  • A.K. Lund, J. Lemmich, A. Adsersen , and C.E. Olsen , Phytochemistry 44 (4) (1997) 679–681.

    Olsen C.E. , '' (1997 ) 44 Phytochemistry : 679 -681 .

  • G. Hussein, H. Miyashiro, N. Nakamura, M. Hattori, N. Kakiuchi , and K. Shimotohno , Phytother. Res. 14 (2000) 510–516.

    Shimotohno K. , '' (2000 ) 14 Phytother. Res. : 510 -516 .

  • V.P. Dixit and S.K. Bhargava , Andrologia 15 (5) (1983) 486–494.

    Bhargava S.K. , '' (1983 ) 15 Andrologia : 486 -494 .

  • O.P. Gupta, M.M. Ali, G.B.J. Ray , and C.K. Atal , Indian J. Phys. Pharm. 21 (1) (1997) 31–39.

    Atal C.K. , '' (1997 ) 21 Indian J. Phys. Pharm. : 31 -39 .

  • U. Zutshi, R.K. Johri , and C.K. Atal , Indian J. Exp. Biol. 27 (7) (1989) 656–657.

    Atal C.K. , '' (1989 ) 27 Indian J. Exp. Biol. : 656 -657 .

  • C.K. Atal, M.A. Siddiqui, U. Zutshi, V. Amla, R.K. Johri, P.G. Rao , and S. Kour , J. Ethnopharmacol. 11 (3) (1984) 309–317.

    Kour S. , '' (1984 ) 11 J. Ethnopharmacol. : 309 -317 .

  • M. Chitra, E. Sukumar, V. Suja , and C.S. Devi , Chemotherapy 40 (2) (1994) 109–113.

    Devi C.S. , '' (1994 ) 40 Chemotherapy : 109 -113 .

  • The Validation of Analytical Procedures Used in the Examination of Pharmaceutical Materials, WHO/Pharm. 89541, Rev 2 Genf, 1989.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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