JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 17: Issue 2
TLC/HPTLC and HPLC methods for monitoring microbial transformation processes
Authors:
Balázs Erdélyi IVAX Drug Research Institute Ltd P.O. Box 82 Budapest Hungary

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,
Antal Szabó IVAX Drug Research Institute Ltd P.O. Box 82 Budapest Hungary

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László Birincsik IVAX Drug Research Institute Ltd P.O. Box 82 Budapest Hungary

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Gábor Seres IVAX Drug Research Institute Ltd P.O. Box 82 Budapest Hungary

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János Salát IVAX Drug Research Institute Ltd P.O. Box 82 Budapest Hungary

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József Ivanics IVAX Drug Research Institute Ltd P.O. Box 82 Budapest Hungary

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Attila Kónya IVAX Drug Research Institute Ltd P.O. Box 82 Budapest Hungary

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Pages:
132–136
Online Publication Date:
11 Mar 2007
Publication Date:
01 Apr 2004
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.17.2004.2.9
Keywords:
Biotransformation; Resin-content medium sampling; HPLC chiral analysis; HPTLC; Enantioselective reduction; Compactin hydroxylation
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TLC methods have been developed for monitoring microbial enantioselective reduction and regioselective oxidation. The work discussed in this paper focused on preparation of aromatic chiral alcohols by bioreduction of their respective acetone derivatives by biocatalysis using yeast strains in medium containing added resin. A simple method and suitable device were developed for sampling from such heterogeneous fermentation media. Methods and chromatograms are presented for quantitation by TLC and for enantiomer ratio determination by HPLC. With regard to oxidation procedures, a TLC method was established for monitoring compactin hydroxylation during fermentation processes — as a representative example. Improvement of the method and use of HPTLC plates resulted in high resolution and separation capacity equal to that of the HPLC method. By means of the method by-products usually formed as minor components in the biotransformation could also be detected. Repeated clogging of the HPLC column as a result of impurities usually present in considerable amounts in fermentation samples is another reason for using TLC for analysis of microbial media.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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