JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 17: Issue 5
Validation and application of an HPTLC method for the determination of parthenolide in feverfew herbal products
Author:
Ehab Abourashed King Saud University Department of Pharmacognosy, College of Pharmacy P.O. Box 2457 Riyadh Saudi Arabia 11451

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Pages:
375–378
Online Publication Date:
08 Mar 2007
Publication Date:
01 Oct 2004
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.17.2004.5.11
Keywords:
HPTLC; Densitometry; Parthenolide; Feverfew; Herbal products; Quantitative analysis; Validation
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A high-performance thin-layer chromatographic method for determination of parthenolide has been validated for recovery, accuracy, specificity, and precision and applied to four feverfew products. Chromatography was performed on silica gel plates with ethyl acetate- n -hexane, 3 + 2 ( v/v ) as mobile phase. Parthenolide was visualized by dipping plates into p -anisaldehyde reagent and heating at 105°C for 5 min. Densitometric quantification of analyte spots was achieved by scanning the plates at λ = 543 nm after derivatization. The method is precise with CV [%] < 5.00; calibration recovery = 101.12 ± 4.11% and overall accuracy = 101.14 ± 4.47%. The levels of parthenolide in the products analyzed ranged from 0.03 to 0.24%.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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