Authors:
Ananya Banerjee S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

Search for other papers by Ananya Banerjee in
Current site
Google Scholar
PubMed
Close
,
Ramesh Sane S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

Search for other papers by Ramesh Sane in
Current site
Google Scholar
PubMed
Close
,
Kiran Mangaonkar S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

Search for other papers by Kiran Mangaonkar in
Current site
Google Scholar
PubMed
Close
,
Sunita Shailajan S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

Search for other papers by Sunita Shailajan in
Current site
Google Scholar
PubMed
Close
,
Abhijeet Deshpande S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

Search for other papers by Abhijeet Deshpande in
Current site
Google Scholar
PubMed
Close
, and
Gunesh Gundi S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

Search for other papers by Gunesh Gundi in
Current site
Google Scholar
PubMed
Close
Restricted access

A sensitive, simple, and accurate high-performance thin-layer chromatographic method has been established for determination of oleanolic acid in whole-plant powder from Oldenlandia corymbosa L. A methanol extract of the powder was used for the experimental work. The concentration of oleanolic acid in whole plant was found to be 1.892 μg g −1 . Separation was performed on aluminum HPTLC plates coated with silica gel 60 F 254 , with dichloromethane-toluene-acetone-methanol, 3 + 4 + 1.5 + 0.3 ( v/v ), as mobile phase. After development, plates were treated with Liebermann-Burchard reagent and detection and quantification were performed by densitometry at λ = 529 nm. Detection and quantitation limits were 0.1 μg and 0.5 μg, respectively. Oleanolic acid response was linear over the range 1 to 9 μg. The validated HPTLC method can be used for routine quality-control analysis of Oldenlandia corymbosa L. whole-plant powder and for quantitative determination of oleanolic acid.

  • H. Tokuda, H. Ohigashi, K. Koshimizu , and Y. Ito , Cancer Lett. 33 (1986) 279–285.

    Ito Y. , '' (1986 ) 33 Cancer Lett. : 279 -285 .

  • Y. Muto, M. Ninomiya , and H. Fujiki , Jpn. J. Clin. Oncol. 20 (1990) 219–224.

    Fujiki H. , '' (1990 ) 20 Jpn. J. Clin. Oncol. : 219 -224 .

  • M. Ishida, T. Okubo, K. Koshimizu, H. Daito, H. Tokuda, T. Kin, T. Yamamoto , and N. Yamazaki , Chem. Abstr. 113 (1990) 12173y.

  • H.Y. Hsu , Cancer Det. Prev. 22 (1998) (Supplement 1).

  • J.C. Touchstone , Practice of Thin-Layer Chromatography, John Wiley and Sons, USA, 1992.

    Touchstone J.C. , '', in Practice of Thin-Layer Chromatography , (1992 ) -.

  • J. Sherma and B. Fried , Handbook of Thin-Layer Chromatography, Chromatographic Science Series, Marcel Dekker, NY, 1996.

    Fried B. , '', in Handbook of Thin-Layer Chromatography, Chromatographic Science Series , (1996 ) -.

  • A.K.S Jardine, J.D. Macfarlane , and C.S. Greeensted , Statistical Methods for Quality Control, IBM Press, UK, 1975.

    Greeensted C.S. , '', in Statistical Methods for Quality Control , (1975 ) -.

  • P.D. Sethi and D. Charegaonkar , Identification of Drugs in Pharmaceutical Formulations by TLC, CBS India, 1999.

    Charegaonkar D. , '', in Identification of Drugs in Pharmaceutical Formulations by TLC , (1999 ) -.

  • E. Stahl , Thin-Layer Chromatography — A Laboratory Handbook, CBS, India, 1969.

    Stahl E. , '', in Thin-Layer Chromatography — A Laboratory Handbook , (1969 ) -.

  • Collapse
  • Expand

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Sep 2023 5 0 0
Oct 2023 7 1 0
Nov 2023 2 2 0
Dec 2023 17 1 0
Jan 2024 10 2 0
Feb 2024 19 1 0
Mar 2024 0 0 0