JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 19: Issue 107
Quantitation of oleanolic acid in Oldenlandia corymbosa L. whole-plant powder by high-performance thin-layer chromatography
Authors:
Ananya Banerjee S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

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Ramesh Sane S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

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Kiran Mangaonkar S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

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Sunita Shailajan S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

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Abhijeet Deshpande S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

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Gunesh Gundi S.P. Mandali’s Ramnarain Ruia College Analytical Chemistry Laboratory Matunga, Mumbai 400 019 India

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Pages:
68–72
Online Publication Date:
11 Mar 2007
Publication Date:
01 Feb 2006
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.19.2006.1.12
Keywords:
HPTLC; Oleanolic acid; Oldenlandia corymbosa L; Silica gel 60 F254; Derivatization; Densitometry
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A sensitive, simple, and accurate high-performance thin-layer chromatographic method has been established for determination of oleanolic acid in whole-plant powder from Oldenlandia corymbosa L. A methanol extract of the powder was used for the experimental work. The concentration of oleanolic acid in whole plant was found to be 1.892 μg g −1 . Separation was performed on aluminum HPTLC plates coated with silica gel 60 F 254 , with dichloromethane-toluene-acetone-methanol, 3 + 4 + 1.5 + 0.3 ( v/v ), as mobile phase. After development, plates were treated with Liebermann-Burchard reagent and detection and quantification were performed by densitometry at λ = 529 nm. Detection and quantitation limits were 0.1 μg and 0.5 μg, respectively. Oleanolic acid response was linear over the range 1 to 9 μg. The validated HPTLC method can be used for routine quality-control analysis of Oldenlandia corymbosa L. whole-plant powder and for quantitative determination of oleanolic acid.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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