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Elena Tamburini University of Ferrara Agro-tech and Pharmaceutical Research Laboratory, Department of Evolutive Biology Ferrara Italy

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Tatiana Bernardi University of Ferrara Biocatalysis and Biotransformation Laboratory, Chemistry Department Via L. Borsari 46 44100 Ferrara Italy

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Ercolina Bianchini University of Ferrara Biocatalysis and Biotransformation Laboratory, Chemistry Department Via L. Borsari 46 44100 Ferrara Italy

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Paola Pedrini University of Ferrara Agro-tech and Pharmaceutical Research Laboratory, Department of Evolutive Biology Ferrara Italy

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An OPLC method has been established for separation and quantification of eight carbohydrates (glucose, fructose, galactose, lactose, sucrose, raffinose, 1-kestose, nystose, and fructosyl-nystose), which might be simultaneously present in a carbon-rich medium to be fermented by a strain of B. adolescentis . The separation was performed in approximately 33 min on aluminum foil-backed silica gel OPLC-HPTLC plates, using overrunning elution, with acetone-acetonitrile 85:15 as mobile phase. Before performing quantitative analyses, the method was optimized to minimize matrix effects caused by the presence of all the non-carbohydrate components dissolved in the broth. Although, when potentially relevant interferences are present a matrix, the method of standard additions is commonly employed, we believe it is excessively time-consuming, because it involves several stages, when applied in planar chromatography. We propose an alternative validated procedure which also enables reliable quantitative analysis.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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