JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 22: Issue 3
HPTLC method for analysis of withaferin-A in Ashwagandha (Withania somnifera)
Authors:
Preeti Nayak Jawaharlal Nehru Krishi Vishwa Vidyalaya Department of Crop and Herbal Physiology, College of Agriculture Jabalpur M.P 482004 India

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Sunil Upadhyaya Jawaharlal Nehru Krishi Vishwa Vidyalaya Department of Crop and Herbal Physiology, College of Agriculture Jabalpur M.P 482004 India

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Anubha Upadhyaya Jawaharlal Nehru Krishi Vishwa Vidyalaya Department of Crop and Herbal Physiology, College of Agriculture Jabalpur M.P 482004 India

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Pages:
197–200
Online Publication Date:
18 Jun 2009
Publication Date:
01 Jun 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.22.2009.3.7
Keywords:
HPTLC; Densitometric; Withania somnifera ; Withaferin-A
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A densitometric high-performance thin layer chromatographic (HPTLC) method has been established for analysis of withaferin-A in Withania somnifera . The analyte was extracted with methanol. Withaferin-A standard and sample was spotted by use of a sample applicator. The plates were developed with toluene-ethyl acetate-formic acid 5:5:1 as mobile phase. Quantitative evaluation was performed by measuring the absorbance of the analyte zones at 200 nm in reflectance mode. The method was shown to have the selectivity, accuracy, precision, and high sample throughout useful for routine analysis of the preparation in industrial quality control and regulatory laboratories.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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