JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 3
Simultaneous HPTLC analysis of E-guggulsterone, Z-guggulsterone, 11-keto-β-boswellic acid, and 3-acetyl-11-keto-β-boswellic acid in an anti-arthritic formulation
Authors:
Nitin Shelke R.C. Patel Institute of Pharmaceutical Education and Research Bioecology Laboratory, Department of Pharmacognosy Shirpur 425405 India

Search for other papers by Nitin Shelke in
Current site
Google Scholar
PubMed Close
,
Vinodkumar Kuber Analytical R&D, Tulip Lab Pvt. Ltd. Plot No-F-20/21, MIDC Ranjangaon Tal-Shirur, Dist-Pune 412220 India

Search for other papers by Vinodkumar Kuber in
Current site
Google Scholar
PubMed Close
,
Shrinivas Bhope Analytical R&D, Tulip Lab Pvt. Ltd. Plot No-F-20/21, MIDC Ranjangaon Tal-Shirur, Dist-Pune 412220 India

Search for other papers by Shrinivas Bhope in
Current site
Google Scholar
PubMed Close
, and
Ramchandra Jadhav R.C. Patel Institute of Pharmaceutical Education and Research Bioecology Laboratory, Department of Pharmacognosy Shirpur 425405 India

Search for other papers by Ramchandra Jadhav in
Current site
Google Scholar
PubMed Close
Pages:
242–247
Online Publication Date:
02 Jun 2011
Publication Date:
01 Jun 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.3.13
Keywords:
E-Guggulsterone; Z-Guggulsterone; 11-Keto-β-boswellic acid; 3-Acetyl-11-keto-β-boswellic acid; HPTLC
Restricted access

A simple, precise, and rapid HPTLC method has been established for simultaneous analysis of E-guggulsterone, Z-guggulsterone, 11-keto-β-boswellic acid (11-KBA), and 3-acetyl-11-keto-β-boswellic acid (A-11-KBA) in an anti-arthritic formulation. The method involves densitometric evaluation of E and Z-guggulsterone, 11-KBA and A-11-KBA after their resolution on pre-coated silica gel 60F254 plates with n-hexane-chloroform-ethyl acetate-methanol 10:3:3:1 (ν/ν) as mobile phase. Detection was at 254 nm. Good resolution was achieved, with RF 0.61 ± 0.03, 0.68 ± 0.03, 0.28 ± 0.03, and 0.39 ± 0.03 for E and Z-guggulsterone 11-KBA, and A-11-KBA, respectively. The method was validated for specificity, linearity, precision, accuracy, and robustness. The method was linear in the range 10–90 ng per band for E and Z-guggulsterone and 50–450 ng per band for 11-KBA and A-11-KBA. Recovery at 80, 100, and 120% was 96.93 ± 0.27, 97.39 ± 0.22, 97.60 ± 0.61, and 97.19 ± 0.41% for E- and Z-guggulsterone, 11-KBA, and A-11-KBA, respectively. The method is simple, specific, precise, accurate, reproducible, and gives results comparable with those from an HPLC method.

  • A.D. Woolf, B. Pfleger, Bull. World Health Org. 81 (2003) 646–656.

    Pfleger B. , '' (2003 ) 81 Bull. World Health Org. : 646 -656 .

  • R.J. Smith, Drug Discov. Today 10 (2005) 1598–1606.

    Smith R.J. , '' (2005 ) 10 Drug Discov. Today : 1598 -1606 .

  • J. Rao, K. Mihaliak, K. Kroenke, J. Bradley, W. Tiernery, M. Weinberger, Ann. Intern. Med. 131 (1999) 409–416.

    Weinberger M. , '' (1999 ) 131 Ann. Intern. Med. : 409 -416 .

  • K.M. Nadkarni, Indian Materia Medica, Vol. 1, Popular Publications, Bombay, 1982, p. 167–170.

    Nadkarni K.M. , '', in Indian Materia Medica, Vol. 1 , (1982 ) -.

  • V.D. Patil, V.R. Nayak, S. Dev, Tetrahedron 28 (1972) 2341.

    Dev S. , '' (1972 ) 28 Tetrahedron : 2341 -.

  • A.G. Bajaj, S. Dev, Tetrahedron 38 (1982) 2949–2954.

    Dev S. , '' (1982 ) 38 Tetrahedron : 2949 -2954 .

  • S. Dev, Sci. Age 5 (1987) 13–18.

    Dev S. , '' (1987 ) 5 Sci. Age : 13 -18 .

  • R.B. Arora, V. Kapoor, S.K. Gupta, R.C. Sharma, Indian J. Exp. Biol. 9 (1971) 403–404.

    Sharma R.C. , '' (1971 ) 9 Indian J. Exp. Biol. : 403 -404 .

  • B.B. Singh, L.C. Mishra, S.P. Vinjamury, N. Aquilina, V.J. Singh, N. Shepard, Altern. Ther. Health Med. 9 (2003) 74–79.

    Shepard N. , '' (2003 ) 9 Altern. Ther. Health Med. : 74 -79 .

  • K.R. Kirtikar, B.D. Basu, Indian medicinal plants, Vol. 1, 2nd edn., International Book Distributors, Dehradun, 1995, 521–529.

    Basu B.D. , '', in Indian medicinal plants, Vol. 1 , (1995 ) -.

  • G.K. Chatterjee, S.D. Pal, Indian Drugs 21 (1984) 431.

    Pal S.D. , '' (1984 ) 21 Indian Drugs : 431 -.

  • V.K. Sethi, B. Mahajan, S.C. Taneja, K.L. Dhar, Phytochemistry 39 (1995) 453–455.

    Dhar K.L. , '' (1995 ) 39 Phytochemistry : 453 -455 .

  • H. Safayhi, T. Mack, J. Sabieraj, M.I. Anazodo, L.R. Subramanian, H.P.T. Ammon, J. Pharmacol. Exp. Biol. 261 (1992) 1143.

    Ammon H.P.T. , '' (1992 ) 261 J. Pharmacol. Exp. Biol. : 1143 -.

  • B.S. Lakshmi, B. Gayathri, N. Manjula, K.S. Vinaykumar, A. Balakrishnan, Int. Immunopharmacol. 7 (2007) 473–482.

    Balakrishnan A. , '' (2007 ) 7 Int. Immunopharmacol. : 473 -482 .

  • M.L. Sharma, A. Kaul, A. Khajuria, S. Singh, G.B. Singh, Phytother. Res. 10 (1996) 107–112.

    Singh G.B. , '' (1996 ) 10 Phytother. Res. : 107 -112 .

  • T. Huang, V. Badmaev, Y. Ding, Y. Liu, J.G. Xie, C.T. Ho, Biofactors 3 (2000) 225–230.

    Ho C.T. , '' (2000 ) 3 Biofactors : 225 -230 .

  • R.C. Gupta, N. Verma, S.K. Singh, J. Chromatogr. B 708 (1998) 243–248.

    Singh S.K. , '' (1998 ) 708 J. Chromatogr. B : 243 -248 .

  • M.L. Sharma, S. Bani, G.B. Singh, Int. J. Immunopharm. 11 (1989) 647–652.

    Singh G.B. , '' (1989 ) 11 Int. J. Immunopharm. : 647 -652 .

  • L. Lao, A.Y. Fan, R.X. Zhang, A.N. Zhou, L.B. Wang, K.D. Moudgil, D.Y.W. Lee, Z.Z. Ma, W.Y. Zhang, B.M. Berman, J. Ethnopharmacol. 101 (2005) 104–109.

    Berman B.M. , '' (2005 ) 101 J. Ethnopharmacol. : 104 -109 .

  • R.C. Pandey, B. Mesrob, C. Nesbitt, R. Misra, J. Chromatogr. B 720 (1998) 189–196.

    Misra R. , '' (1998 ) 720 J. Chromatogr. B : 189 -196 .

  • I. Gupta, V. Gupta, A. Parihar, S. Gupta, R. Ludtke, H. Safayhi, H.P.T. Ammon, Eur. J. Med. Res. 3 (1998) 511–514.

    Ammon H.P.T. , '' (1998 ) 3 Eur. J. Med. Res. : 511 -514 .

  • K.R. Mahadik, H. Agrawal, N. Kaul, A.R. Paradkar, J. Pharm. Biomed. Anal. 36 (2004) 23–31.

    Paradkar A.R. , '' (2004 ) 36 J. Pharm. Biomed. Anal. : 23 -31 .

  • K.R. Mahadik, H. Agrawal, N. Kaul, A.R. Paradkar, J. Pharmaceut. Biomed. 36 (2004) 33–41.

    Paradkar A.R. , '' (2004 ) 36 J. Pharmaceut. Biomed. : 33 -41 .

  • T. Simmet, W. Zugmaier, B. Buchele, J. Chromatogr. B 791 (2003) 21–30.

    Buchele B. , '' (2003 ) 791 J. Chromatogr. B : 21 -30 .

  • U. Bahr, M. Wurglics, M.A. Tawab, A. Kaunzinger, M. Karas, M. Schubert-Zsilavecz, J. Chromatogr. B 761 (2001) 221–227.

    Schubert-Zsilavecz M. , '' (2001 ) 761 J. Chromatogr. B : 221 -227 .

  • S.A. Shah, I.S. Rathod, B.N. Suhagia, D.A. Patel, V.K. Parmar, B.K. Shah, V.M. Vaishnavi, J. Chromatogr. B 848 (2007) 232–238.

    Vaishnavi V.M. , '' (2007 ) 848 J. Chromatogr. B : 232 -238 .

  • International Conference on Harmonization (ICH) Guideline, Q2 (R1), Validation of Analytical Procedure: Text and Methodology, in: Proceedings of the International Conference on Harmonization, Geneva, Nov 2005.

  • Collapse
  • Expand
Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Mar 2024 2 0 0
Apr 2024 16 0 0
May 2024 0 0 0
Jun 2024 8 0 0
Jul 2024 2 0 0
Aug 2024 16 0 0
Sep 2024 6 0 0