JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 4
HPTLC method for quantification of isovitexin in the whole-plant powder of Enicostemma littorale Blume
Authors:
Laxman Sawant SVKM’s NMIMS School of Pharmacy and Technology Management Vile Parle (W), Mumbai 400056 India

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Bala Prabhakar SVKM’s NMIMS School of Pharmacy and Technology Management Vile Parle (W), Mumbai 400056 India

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, and
Nancy Pandita SVKM’s NMIMS School of Pharmacy and Technology Management Vile Parle (W), Mumbai 400056 India

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Pages:
301–305
Online Publication Date:
13 Aug 2011
Publication Date:
01 Aug 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.4.5
Keywords:
Enicostemma littorale Blume; HPTLC; Densitometry
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A sensitive and accurate high-performance thin-layer chromatographic method has been developed, validated, and used for quantification of isovitexin in dried whole-plant powder of Enicostemma littorale Blume. Chromatographic separation was performed on silica gel plates with acetonitrile-water, 6.0:4.0 (ν/ν), as mobile phase. Detection and quantitation were performed by densitometry, with a deuterium lamp, at 350 nm. The response to isovitexin reference standard was linear in the concentration range of 100–400 ng per band. The method was validated for precision and accuracy. The relative standard deviation for instrumental precision, intra-assay precision, and intermediate precision was <2%. The percentage recovery was 99.76. This method can be used for routine quality control analysis of the whole-plant powder of E. littorale Blume.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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