Authors:
Amanda Seemungal Kingston University School of Life Sciences Kingston upon Thames Surrey KT1 2EE UK

Search for other papers by Amanda Seemungal in
Current site
Google Scholar
PubMed
Close
,
Andrea Petróczi Kingston University School of Life Sciences Kingston upon Thames Surrey KT1 2EE UK

Search for other papers by Andrea Petróczi in
Current site
Google Scholar
PubMed
Close
, and
Declan Naughton Kingston University School of Life Sciences Kingston upon Thames Surrey KT1 2EE UK

Search for other papers by Declan Naughton in
Current site
Google Scholar
PubMed
Close
Restricted access

The potential health benefits of wine are frequently attributed to the antioxidant properties of its phenolic constituents. In this study, the antioxidant efficacies of five phenolic components of a red wine were assessed in the presence of hydrogen peroxide (H2O2) and metal ions ([Fe3+], [Fe2+], [Cu2+]), using functional thin-layer chromatography (TLC). The pH of the red wine sample was adjusted to 2.0 without prior extraction. Chromatographic separation was performed using silica gel 60 F254 TLC plates with toluene-ethyl acetate-formic acid 30:25:5 (ν/ν) as mobile phase. Five phenolic components (one flavonol and four phenolic acids) were identified. In independent experiments, the red wine was challenged with a range of oxidant systems comprising H2O2, redox-active metals (Fe3+ and Cu2+), and hydroxyl radical generators (Fe2+-H2O2 and Cu2+-H2O2). The results showed that all phenolic compounds gradually diminished in a concentration- and time-dependent manner. All systems tested showed that quercetin, caffeic acid, and gallic acid possessed the highest antioxidant efficacies, while chlorogenic acid and p-coumaric acid exhibited lower antioxidant capacities. Based on these results, the ranking order of the antioxidant efficacies of the phenolic components in the red wine sample was as follows: quercetin > caffeic acid > gallic acid > p-coumaric acid > chlorogenic acid. This is the first application of functional TLC to delineate the antioxidant profile of red wine in the presence of different oxidant systems involving redox-active metals and H2O2, and to rank the individual components according to their antioxidant efficacies.

  • A. Waterhouse, Ann. NY Acad. Sci. 957 (2002) 21–36.

    Waterhouse A. , '' (2002 ) 957 Ann. NY Acad. Sci. : 21 -36 .

  • A. Kondrashov, R. Ševčík, H. Benáková, M. Koštířová, S. Štipek, Eur. J. Clin. Nutr. Metab. 4 (2009) e41–e46.

    Štipek S. , '' (2009 ) 4 Eur. J. Clin. Nutr. Metab. : e41 -e46 .

  • B. Halliwell, Free Radic. Biol. Med. 46 (2009) 531–542.

    Halliwell B. , '' (2009 ) 46 Free Radic. Biol. Med. : 531 -542 .

  • A. Fisher, D.P. Naughton, Proc. Indian Natl. Sci. Acad. B69 (2003) 453–460.

    Naughton D.P. , '' (2003 ) B69 Proc. Indian Natl. Sci. Acad. : 453 -460 .

  • D.P. Naughton, M. Grootveld, Bioorg. Med. Chem. Lett. 11 (2001) 2573–2575.

    Grootveld M. , '' (2001 ) 11 Bioorg. Med. Chem. Lett. : 2573 -2575 .

  • A.E.O. Fisher, D.P. Naughton, Bioorg. Med. Chem. Lett. 13 (2003) 1733–1735.

    Naughton D.P. , '' (2003 ) 13 Bioorg. Med. Chem. Lett. : 1733 -1735 .

  • D.P. Naughton, T. Nepusz, A. Petroczi, Met. Ions Life Sci. 8 (2011) 107–132.

    Petroczi A. , '' (2011 ) 8 Met. Ions Life Sci. : 107 -132 .

  • N. Sugihara, M. Ohnishi, M. Imamura, K. Furuno, J. Health Sci. 47 (2001) 99–106.

    Furuno K. , '' (2001 ) 47 J. Health Sci. : 99 -106 .

  • M. Soobrattee, V. Neergheen, A. Luximon-Ramma, O. Aruoma, T. Bahorun, Mutat. Res. 579 (2005) 200–213.

    Bahorun T. , '' (2005 ) 579 Mutat. Res. : 200 -213 .

  • K.E. Heim, A.R. Tagliaferro, D.J. Bobilya, J. Nutr. Biochem. 13 (2002) 572–584.

    Bobilya D.J. , '' (2002 ) 13 J. Nutr. Biochem. : 572 -584 .

  • D. Malešev, V. Kuntić, J. Serb. Chem. Soc. 72 (2007) 921–939.

    Kuntić V. , '' (2007 ) 72 J. Serb. Chem. Soc. : 921 -939 .

  • I. Roussis, I. Lambropoulos, P. Tzimas, A. Gkoulioti, V. Marinos, D. Tsoupeis, I. Boutaris, J. Food Comp. Anal. 21 (2008) 614–621.

    Boutaris I. , '' (2008 ) 21 J. Food Comp. Anal. : 614 -621 .

  • F. Alen-Ruiz, M. Garcia-Falcon, M.C. Perez-Lamela, E. Martinez-Carballo, J. Simal-Gandara, Food Chem. 113 (2009) 53–60.

    Simal-Gandara J. , '' (2009 ) 113 Food Chem. : 53 -60 .

  • M.S. Fernández-Pachón, D. Villańo, M.C. García-Parrilla, A.M. Troncoso, Anal. Chim. Acta 513 (2004) 113–118.

    Troncoso A.M. , '' (2004 ) 513 Anal. Chim. Acta : 113 -118 .

  • F. Cimino, V. Sulfaro, D. Trombetta, A. Saija, A. Tomaino, Food Chem. 103 (2007) 75–81.

    Tomaino A. , '' (2007 ) 103 Food Chem. : 75 -81 .

  • V. Rastija, A. Mornar, I. Jasprica, G. Srečnik, M. Medišarić, Food Chem. 115 (2009) 54–60.

    Medišarić M. , '' (2009 ) 115 Food Chem. : 54 -60 .

  • V. Rastija, A. Mornar, I. Jasprica, G. Srečnik, M. Medišarić, J. Planar Chromatogr. 17 (2004) 26–31.

    Medišarić M. , '' (2004 ) 17 J. Planar Chromatogr. : 26 -31 .

  • C. Cimpoiu, A. Hosu, R. Briciu, V. Miclaus, J. Planar Chromatogr. 20 (2007) 407–410.

    Miclaus V. , '' (2007 ) 20 J. Planar Chromatogr. : 407 -410 .

  • F. Fang, J.-M. Li, Q.-H. Pan, W.-D. Huang, Food Chem. 101 (2007) 428–433.

    Huang W.-D. , '' (2007 ) 101 Food Chem. : 428 -433 .

  • S. Gómez-Alonso, E. García-Romero, I. Hermosín-Gutiérrez, J. Food Comp. Anal. 20 (2007) 618–626.

    Hermosín-Gutiérrez I. , '' (2007 ) 20 J. Food Comp. Anal. : 618 -626 .

  • P. Pohl, Trends Anal. Chem. 26 (2007) 941–949.

    Pohl P. , '' (2007 ) 26 Trends Anal. Chem. : 941 -949 .

  • W. Zheng, S.Y. Wang, J. Agric. Food Chem. 51 (2003) 502–509.

    Wang S.Y. , '' (2003 ) 51 J. Agric. Food Chem. : 502 -509 .

  • J.H. Chen, C.-T. Ho, J. Agric. Food Chem. 45 (1997) 2374–2378.

    Ho C.-T. , '' (1997 ) 45 J. Agric. Food Chem. : 2374 -2378 .

  • M. Andjelković, J. Van Camp, B. De Meulenaer, G. Depaemelaere, C. Socaciu, M. Verloo, R. Verhe, Food Chem. 98 (2006) 23–31.

    Verhe R. , '' (2006 ) 98 Food Chem. : 23 -31 .

  • F. Natella, M. Nardini, M. Di Felice, C. Scaccini, J. Agric. Food Chem. 47 (1999) 1453–1459.

    Scaccini C. , '' (1999 ) 47 J. Agric. Food Chem. : 1453 -1459 .

  • Collapse
  • Expand

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2023 7 1 0
Dec 2023 24 1 0
Jan 2024 23 4 0
Feb 2024 25 2 0
Mar 2024 25 0 0
Apr 2024 5 0 0
May 2024 0 0 0