JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 6
Development and validation of an HPTLC method for the analysis of oleanolic acid from the roots of Helicteres isora Linn.
Authors:
Pinal Harde C.K. Pithawalla Institute of Pharmaceutical Science and Research Dumas Road Surat 395007 Gujarat India

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,
Dinesh Shah Maliba Pharmacy College Bardoli Gujarat India

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,
Bhanu Suhagia L.M. College of Pharmacy Ahmedabad Gujarat India

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, and
Mamta Shah L.M. College of Pharmacy Ahmedabad Gujarat India

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Pages:
503–506
Online Publication Date:
02 Dec 2011
Publication Date:
01 Dec 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.6.9
Keywords:
High-performance thin-layer chromatography (HPTLC); Oleanolic acid; Marker; Sapogenins; Helicteres isora
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A sensitive and reliable high-performance thin-layer chromatographic (HPTLC) method has been developed for the quantitative determination of oleanolic acid in the dried roots of Helicteres isora Linn. Total sapogenins were isolated from the roots, and their alcoholic extract was applied on silica gel G 60 F254 plates with toluene-ethyl acetate-glacial acetic acid, 7:3:0.1 (ν/ν/ν) as mobile phase. Detection and quantification were performed by densitometric scanning at 529 nm. The accuracy of the method was confirmed by conducting recovery studies at three different levels using the standard addition method. The average recovery of oleanolic acid was found to be 98.98%. The proposed HPTLC method provides good resolution of oleanolic acid from other constituents present in sapogenin extract of dried roots of H. isora and can be used for quantification of oleanolic acid present in the extract. The method is simple, accurate, and precise.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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